Food Engineering / Gıda Mühendisliği
Permanent URI for this collectionhttps://hdl.handle.net/11147/12
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Article Citation - WoS: 11Citation - Scopus: 10Redefining methods for augmenting lactic acid bacteria robustness and phenyllactic acid biocatalysis: Integration valorizes simplicity(Taylor & Francis, 2022) Meruvu, HarithaThe production of phenyllactic acid (PLA) has been reported by several researchers, but so far, no mention has been made of augmented PLA production using an orchestrated assembly of simple techniques integrated to improve lactic acid bacteria (LAB) metabolism for the same. This review summarizes sequentially tailoring LAB growth and metabolism for augmented PLA catalysis through several strategies like monitoring LAB sustenance by choosing appropriate starter PLA-producing LAB strains isolated from natural environments, with desirably fastidious growth rates, properties like acidification, proteolysis, bacteriophage-resistance, aromatic/texturing-features, etc.; entrapping chosen LAB strains in novel cryogels and/or co-cultivating two/more LAB strains to improve their biotransformation potential and promote growth dependency/sustainability; adopting adaptive evolution methods designed to improve LAB strains under selection pressure inducing desired phenotypes tolerant to stress factors like heat, salt, acid, and solvent; monitoring physico-chemical LAB fermentation factors like temperature, pH, dissolved oxygen content, enzymes, and cofactors for PLA biosynthesis; and modulating purification/downstream processes to extract substantial PLA yields. This review paper serves as a comprehensive preliminary guide that can evoke a strategic experimental plan to produce industrial-scale PLA yields using simple techniques orchestrated together in the pursuit of conserving time, effort, and resources.Article Citation - WoS: 16Citation - Scopus: 18Microencapsulation of a Potential Probiotic Lactiplantibacillus Pentosus and Its Impregnation Onto Table Olives(Elsevier, 2022) Elvan, Menşure; Baysal, Ayşe Handan; Harsa, Hayriye ŞebnemThis study aimed to demonstrate some probiotic properties of Lactiplantibacillus pentosus NRRL B-227 and impregnate the strain onto the table olive surfaces. In this respect, antioxidative ability, antibiotic resistance, and survivability after simulated digestion tests were carried out. Microencapsulation was performed using xylan and whey protein concentrate (WPC) using the water-in-oil emulsion technique to maintain cell viability. A vacuum impregnation process was performed to coat olive surfaces with L. pentosus. This strain demonstrated 71.6% DPPH radical scavenging activity and exhibited paramount resistance to antibiotics. The viable cell count of microencapsulated L. pentosus was found as 8 log CFU/g after 72 weeks of storage. After exposure to the simulated oral phase, 1-log reduction was detected, and gastric phase conditions led to a 3-log reduction of viability of microencapsulated as well as non-microencapsulated cells. The viability of microencapsulated L. pentosus on the surface of olives was also evaluated for one-month, and viable cell count was ≥6 log CFU/g. In the light of these findings, L. pentosus with antibiotic and digestion fluids resistant and antioxidant properties were successfully microencapsulated within xylan-WPC complex. Table olives can be considered as a suitable carrier for beneficial microorganisms that satisfies with the expectations of regulations for functional foods.Article Citation - WoS: 35Citation - Scopus: 42Lactic Acid Bacteria: Isolation–characterization Approaches and Industrial Applications(Taylor & Francis, 2022) Meruvu, Haritha; Tellioğlu Harsa, ŞebnemThe current state-of-art research pertaining to lactic acid bacteria (LAB) calls for the screening and isolation of robust LAB strains to achieve holistic exploitation of LAB and their metabolites of marketable importance. Hence it is imperative to comprehend LAB sources, growth requisites, isolation and characterization strategies necessary for featured cataloging and appropriate culturing. This review comprehensively describes various growth media and biomasses used for supporting LAB sustenance, assay procedures needed for the isolation and characterization of LAB strains, and their application in diverse sectors. The various industrial patents and their summarized claims about novel LAB strains isolated and identified, methods and media (used for detection/screening, isolation, adaptation, culturing, preservation, growth improvement), the techniques and/or methodologies supporting LAB fermentation, and applications of produced industrial metabolites in various market scenarios are detailedArticle Citation - WoS: 15Citation - Scopus: 17Effect of Different Microencapsulating Materials on the Viability of S. Thermophilus Ccm4757 Incorporated Into Dark and Milk Chocolates(Elsevier Ltd., 2021) Öztürk, Burcu; Elvan, Menşure; Özer, Merve; Tellioğlu Harsa, ŞebnemThis study aimed to evaluate the viability and bioaccessibility of Streptococcus thermophilus CCM4757 strain supplemented in dark and milk chocolates during storage period and pass through simulated in vitro gastro-intestinal tract. Microencapsulated and non-microencapsulated S. thermophilus CCM4757 strain added into the chocolates. Emulsion technique was used to microencapsulate cells with various biopolymers; carboxymethylcellulose, pectin, gum arabic, and cellobiose. The microencapsulated S. thermophilus with these coating materials was found to be viable higher than 9 log CFU/g up to 180 days of storage at 4 °C. Microbiological, physicochemical, and sensorial attributes of the chocolates containing microencapsulated and non-microencapsulated S. thermophilus CCM4757 were analyzed. The microencapsulated S. thermophilus showed a good survivability in milk (7.12 log CFU/g) and dark (6.90 log CFU/g) chocolate samples during 180-day storage at 4 °C. Supplementation of S. thermophilus did not affect significantly (P > 0.05) the sensory attributes of the chocolates. The results showed that S. thermophilus CCM4757 exhibited good cell survivability higher than 85% in chocolates under simulated gastro-intestinal fluids. S. thermophilus supplementation into the chocolate protected the viability of cells and did not affect the sensorial characteristics and moisture content of chocolates. The present study demonstrated that the dark and milk chocolates could be used as an important matrix to carry probiotics. © 2021 Elsevier Ltd