Nalbant Aldanmaz, Ayten
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Aldanmaz, Ayten Nalbant
Nalbant, A
Nalbant, A.
Nalbant, Ayten
Nalbant, A
Nalbant, A.
Nalbant, Ayten
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aytennalbant@iyte.edu.tr
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04.03. Department of Molecular Biology and Genetics
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1NO POVERTY
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2ZERO HUNGER
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3GOOD HEALTH AND WELL-BEING
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4QUALITY EDUCATION
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5GENDER EQUALITY
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6CLEAN WATER AND SANITATION
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8DECENT WORK AND ECONOMIC GROWTH
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9INDUSTRY, INNOVATION AND INFRASTRUCTURE
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10REDUCED INEQUALITIES
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11SUSTAINABLE CITIES AND COMMUNITIES
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12RESPONSIBLE CONSUMPTION AND PRODUCTION
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14LIFE BELOW WATER
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15LIFE ON LAND
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16PEACE, JUSTICE AND STRONG INSTITUTIONS
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17PARTNERSHIPS FOR THE GOALS
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11
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829
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6
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Scholarly Output
23
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10
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388198/53812
Supervised MSc Theses
9
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WoS Citation Count
704
Scopus Citation Count
825
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13
WoS Citations per Publication
30.61
Scopus Citations per Publication
35.87
Open Access Source
21
Supervised Theses
9
| Journal | Count |
|---|---|
| PLoS ONE | 3 |
| Turkish Journal of Biology | 3 |
| European Journal of Human Genetics | 1 |
| FEBS Journal | 1 |
| Frontiers in Bioscience, Elite | 1 |
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23 results
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Now showing 1 - 10 of 23
Master Thesis Investigation of Fas/Fas-ligand Interaction in Helper T 17 Cell Functions(01. Izmir Institute of Technology, 2021) Çalışkan, Tufan Utku; Nalbant Aldanmaz, Ayten; Nalbant Aldanmaz, AytenTh17 cells are key players of the adaptive immune system. They mainly take part in responses to extracellular parasites and neutrophil recruitment. They can infiltrate into the inflammation sites and survive for long periods of time. Their malfunction leads to the manifestation of several autoimmune diseases, such as Multiple Sclerosis and Rheumatoid Arthritis. The main reason behind their roles in autoimmune pathogenicity is thought to be their longevity and resistance to apoptosis. One of the main players of apoptosis, Fas, has been found to have non-apoptotic roles and is a candidate for the survival mechanisms of Th17 cells. This study aims to discover possible non-apoptotic roles of the Fas signaling pathway in Th17 cell functions. For this purpose, buffy coats of healthy individuals were used to isolate PBMCs and CD4+CD45RA+ naive T cells were sorted from the PBMCs. Obtained naive T cells were cultured under Th17 polarizing conditions and the expressions of Fas, FasL, TNFR1, and TNF-α have been monitored along with apoptosis. The expression of Fas has been found to significantly increase in the cells cultured under Th17 polarizing conditions. However, there were no FasL and TNFR1 expressions observed. The expression of TNF-α was observed on both the negative culture and Th17 polarizing culture, however, there was no significant difference found. In addition, there was no increase in apoptosis in neither culture. In summary, Fas expression has been found to increase in the cells cultured under Th17 polarizing conditions. Further investigation of possible survival mechanisms, such as NFkB, in these cells can shed light on the effects of the Fas signaling pathway on the longevity of Th17 cellsArticle Citation - WoS: 24Citation - Scopus: 29Il-17, Il-21, and Il-22 Cytokines of T Helper 17 Cells in Cancer(Mary Ann Liebert, 2019) Nalbant, AytenCD4(+) T helper (Th) cells are important regulators of cellular immune response. Newly discovered interleukin (IL)-17-producing CD4(+) T cells are known as T helper 17 cells (Th17). They are distinct subset from the T helper type 1 (Th1) and 2 (Th2) lineages. The differentiation of Th17 cells has been intensively studied; however, the role of Th17 cells in different diseases including cancer is still under investigation. Besides IL-17 family cytokines, Th17 cells produce IL-22, IL-21, and IL-26. The dysregulated function of Th17 cells and their cytokines could contribute to pathology of diseases, including cancer. The role of cytokines of Th17 cells such as IL-17, IL-21, and IL-22 in cancer will be discussed in this review.Article Citation - WoS: 5Citation - Scopus: 6Bacterial Heat Shock Protein Groel (hsp64) Exerts Immunoregulatory Effects on T Cells by Utilizing Apoptosis(Public Library of Science, 2016) Nalbant, Ayten; Kant, MelisAggregatibacter actinomycetemcomitans (Aa) expresses a 64-kDa GroEL protein belonging to the heat shock family of proteins. This protein has been shown to influence human host cells, but the apoptotic capacity of the GroEL protein regarding T cells is not yet known. The purpose of this study was to investigate the ability of A. actinomycetemcomitans GroEL (AaGroEL) protein to induce human peripheral blood T-cell apoptosis. Endogenous, purified AaGroEL protein was used as an antigen. In AaGroEL-treated T cells, the data indicated that phosphatidylserine exposure, an early apoptotic event, was dose- and time-dependent. The AaGroEL-treated T cells were also positive for active caspase-3 in a dose-dependent manner. The rate of AaGroEL-induced apoptosis was suppressed by the addition of the general caspase inhibitor Z-VAD-FMK. Furthermore, cleaved caspase-8 bands (40/36 kDa and 23 kDa) were identified in cells responding to AaGroEL. DNA fragmentation was also detected in the AaGroEL-treated T cells. Overall, we demonstrated that the endogenous GroEL from A. actinomycetemcomitans has the capacity to induce T-cell apoptosis. © 2016 Nalbant, Kant. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Master Thesis Determination of Apoptotic Effects of Clinoptilolite on Human T Lymphocytes(Izmir Institute of Technology, 2008) Uslu, Mehmet Emin; Nalbant Aldanmaz, AytenZeolites are defined as aluminum silicates that have made up of oxygen, aluminum and silicon. SiO4 and Alo4 tetrahedrals are the smallest units that give the specific shape to the molecule. There are more than 40 types of natural and over 150 types of synthetic zeolites occurs and those zeolites are used in agriculture, animal husbandry, architecture, pharmaceuticals and metallurgy. Recent years in literature it was shown that these zeolites can have regulatory effects on immune system. Also it was shown that they can influence the development of cancer and have a role on the expression of tumor suppressor genes. However there is no evidence can be found on how these molecules affect the function of specific cell types in the molecular level and also the mechanism of this effect.In this study the apoptotic effects of clinoptilolite which is a natural zeolite found in Gördes region of Turkey, on human T lymphocytes were studied. T cells were chosen in this study because they are the main players in the immune system. These cells can establish immune regulation and organize immune response. As a result, they are important in pathology and therapy. Peripheral blood mono nuclear cells (PBMCs) in which T cells can be found were isolated from the healthy donors blood by Ficoll Hypaque Gradient Method and then these cells were incubated with clinoptilolite in the RPMI 1640 media. Apoptosis were measured in FacsArray after appropriate immunofluorescent labeling and by agarose electrophoresis technique after DNA fragmentation assay done.Master Thesis Expression of Aquaporin 1, 3 and 4 in T Cell Activation and Apoptosis(Izmir Institute of Technology, 2018) Gelmez, Ayşe Bengisu; Nalbant Aldanmaz, AytenAquaporins (AQPs) are membrane proteins responsible for transporting water, some gases and small solutes such as CO2 and glycerol. Until now, it has been shown that AQP1, 3 and 5 expressed in both B and T lymphocytes of mice, regulate cell volume. However, aquaporin expression involved in activation, proliferation, and differentiation as well as apoptosis of T cells are not well known yet. The goal of this study is to detect the expression level of AQP1, AQP3, and AQP4 in activated and apoptotic T cells. In order to do that, two types of T cells cultured in both condition were utilized. Peripheral Blood Mononuclear Cells (PBMCs) were isolated from Human peripheral blood drawn from healthy donors by ficoll density gradient Centrifugation method. Naive CD4+ T cells were sorted from PBMC. The stimulants generating Th17 were chosen for activation and differentiation of naïve CD4 T cells. Jurkat cell line as a second cell type were activated by PMA/Ionomycin as well as treated by camptothecin for apoptotic processing. Th17 and Jurkat cell cultures were analysed by flow cytometry to measure the rate of both activation and apoptosis. Western Blot was performed to identify expression of AQP 1, 3 and 4. We found a significance between increased expression level of AQP1, 3, and 4 in activated T cells as well as decreased expression level of each three AQPs in apoptotic T cell populations. According to our findings, tested aquaporin proteins may play roles in T cell activation, differentiation, and apoptosis. The scientific significance of this research is that it can fill the gaps about these three functional processes of T cells. Besides, all findings can contribute to treatment of many autoimmune disease like MS which Th17 cells involve in pathogeny.Article Citation - Scopus: 45Genes Associated With T Helper 17 Cell Differentiation and Function(Frontiers Media S.A., 2016) Nalbant, Ayten; Eskier, DoğaInterleukin-17 (IL-17)-producing T helper cells (Th17 cells) constitute a lineage of CD4 effector T helper cells that is distinct from the Th1 and Th2 CD4 phenotypes. In humans, Th17 differentiation is induced in the presence of the cytokines IL-1 beta, IL-6 and TGF beta, whereas IL-23 maintains Th17 survival. Effector human Th17 cells express several cytokines and cell surface markers, including IL-17A, IL-17F, IL-22, IL-26, CCR6 and TNFa. Studies on human cells have revealed that the RORC2 transcription factor plays an effective role in Th17 differentiation. Th17 cells contribute to the host immune response by involving various pathologies, including rheumatoid arthritis, multiple sclerosis and Crohn's disease. However, the full extent of their contribution to diseases is being investigated. The differentiation of Th17 cells is controlled by many transcription factors, including ROR gammat, IRF4, RUNX1, BATF, and STAT3. This review covers the general principles of CD4 T helper differentiation and the known transcription factors that play a role in the recently discovered Th17 cells.Article Citation - WoS: 15Citation - Scopus: 11T Cells in Tumor Microenvironment(SAGE Publications Inc., 2016) Kiraz, Yağmur; Baran, Yusuf; Nalbant, AytenTumors progress in a specific area, which supports its development, spreading or shrinking in time with the presence of different factors that effect the fate of the cancer cells. This specialized site is called “tumor microenvironment” and has a composition of heterogenous materials. The immune cells are also residents of this stromal, cancerous, and inflammatory environment, and their types, densities, or functional differences are one of the key factors that mediate the fate of a tumor. T cells as a vital part of the immune system also are a component of tumor microenvironment, and their roles have been elucidated in many studies. In this review, we focused on the immune system components by focusing on T cells and detailed T helper cell subsets in tumor microenvironment and how their behaviors affect either the tumor or the patient’s outcome. © 2015, International Society of Oncology and BioMarkers (ISOBM).Master Thesis Determination of Human T-Lymphocyte Apoptosis Mediated by Bacterial Heat Shock Protein(Izmir Institute of Technology, 2009) Dinç, Melis; Nalbant Aldanmaz, AytenPeriodontal diseases are the most common inflammatory disease worldwide which caused by the pathogenic organism living in biofilm. Aggregatibacter Actinomycetemcomitans (Aa) is the main player of the periodontitis disease pathology. Although some of the virulence factors of Aa has been identified up to now, its cytotoxic mechanism has not been clearly known yet. Although known virulence factors of Aa; ltx and cdt has been knocked out, the mutant Aa strains have retained the ability to induce apoptosis. Depending on the literature there must be another important virulence factor. 64kDa GroEL protein which is a molecular chaperone and a heat shock protein can be the potential candidate for being a virulence factor. AaGroEL protein has not been studied in terms of apoptosis up to now and it is not known how AaGroEL mediate immune regulation of T cells. In this study AaGroEL protein has been purified by using ATP Affinity chromatography and electroelution methods. After the purification step lps contamination has been removed by detoxi-gel endotoxin removal gel and detected by LAL Assay. Peripheral Blood Mononuclear Cells (PBMCs) were isolated by Ficoll Hypaque Density Gradient Centrifugation method. It was found that AaGroEL protein induces T cell apoptosis in dose and a time dependent manner. AaGroEL protein mediated T cell apoptosis has been detected by plasma membrane changes, activation of caspase-3 and DNA fragmentation. In conclusion, AaGroEL has antigenic properties that effect T lymphocytes by regulating immune response that would play important role in periodontal pathology.Article Citation - WoS: 6Citation - Scopus: 8Deep Sequencing Reveals Two Jurkat Subpopulations With Distinct Mirna Profiles During Camptothecin-Induced Apoptosis(TUBITAK, 2018) Erdoğan, İpek; Coşacak, Mehmet İlyas; Nalbant, Ayten; Akgül, BünyaminMicroRNAs (miRNAs) are small noncoding RNAs of about 19-25 nt that regulate gene expression posttranscriptionally under various cellular conditions, including apoptosis. The miRNAs involved in modulation of apoptotic events in T cells are partially known. However, heterogeneity associated with cell lines makes it difficult to interpret gene expression signatures, especially in cancer-related cell lines. Treatment of the Jurkat T-cell leukemia cell line with the universal apoptotic drug, camptothecin, resulted in identification of two Jurkat subpopulations: one that is sensitive to camptothecin and another that is rather intrinsically resistant. We sorted apoptotic Jurkat cells from nonapoptotic ones prior to profiling miRNAs through deep sequencing. Our data showed that a total of 184 miRNAs were dysregulated. Interestingly, the apoptotic and nonapoptotic subpopulations exhibited distinct miRNA expression profiles. In particular, 6 miRNAs were inversely expressed in these two subpopulations. The pyrosequencing results were validated by real-time qPCR. Altogether, these results suggest that miRNAs modulate apoptotic events in T cells and that cellular heterogeneity requires careful interpretation of miRNA expression profiles obtained from drug-treated cell lines.Master Thesis Cytokine Expression Pattern of Human T Lymphocytes in Response To A. Actinomycetemcomitans Groel Protein(Izmir Institute of Technology, 2009) Saygılı, Tahsin; Nalbant Aldanmaz, AytenActinobacillus (Aggregatibacter) actinomycetemicomitans is a bacterium that plays important role in Periodontitis. Recent publications have correlated AaGroEL (A.actinomycetemcomitans GroEL Protein) protein with periodontal pathologies however, how does this protein affect T lymphocytes and the profile of T lymphocytes. immune response, are not reported yet. Within this thesis project, AaGroEL protein is produced as its recombinant form. The effects of AaGroEL protein, after it is obtained, have been investigated on naive T lymphocytes in peripheral blood. In this research, the proliferative and activating effects of AaGroEL protein on T lymphocytes were determined by investigating the expression of CD25 and CD69 cell surface molecules. AaGroEL protein has an effect on CD4+ T lymphocytes and depending on time, it increases CD25 and CD69 expression. Additionally, it was found that, CD4+CD25+T cells are negative for FOXP3. The cytokines of CD4+ T lymphocytes were profiled. Firstly, the cytokines that were released by PBMC.s on culture supernatant were determined. Following AaGroEL stimulation, IL-6, IL-10, IFNg ve TNF. were secreted to the supernatant. Intracellular cytokine staining was applied to determine the source of those cytokines which was found that CD4+ T cells produce those cytokines as well as nonlymphocytes and B or/and CD8+ cells also secrete them. IL-2 was secreted by CD4+ T lymphocytes only. These findings have demonstrated us; AaGroEL protein activates naive CD4+ T cells towards Th1 response. In conclusion, AaGroEL protein has an antigenic effect on T lymphocytes, regulates immune response and play important role on periodontal pathology.
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