Tekin, Hüseyin Cumhur
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Tekin, Huseyin Cumhur
Tekin, Cumhur
Tekin, H. C.
Tekin, H. Cumhur
Tekin, Huseyin C.
Tekin, Hüseyin C.
Tekin, Cumhur
Tekin, H. C.
Tekin, H. Cumhur
Tekin, Huseyin C.
Tekin, Hüseyin C.
Job Title
Email Address
cumhurtekin@iyte.edu.tr
Main Affiliation
03.01. Department of Bioengineering
Status
Current Staff
ORCID ID
Scopus Author ID
Turkish CoHE Profile ID
Google Scholar ID
WoS Researcher ID
Sustainable Development Goals
1NO POVERTY
0
Research Products
2ZERO HUNGER
3
Research Products
3GOOD HEALTH AND WELL-BEING
25
Research Products
4QUALITY EDUCATION
4
Research Products
5GENDER EQUALITY
0
Research Products
6CLEAN WATER AND SANITATION
7
Research Products
7AFFORDABLE AND CLEAN ENERGY
8
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8DECENT WORK AND ECONOMIC GROWTH
2
Research Products
9INDUSTRY, INNOVATION AND INFRASTRUCTURE
20
Research Products
10REDUCED INEQUALITIES
1
Research Products
11SUSTAINABLE CITIES AND COMMUNITIES
0
Research Products
12RESPONSIBLE CONSUMPTION AND PRODUCTION
4
Research Products
13CLIMATE ACTION
8
Research Products
14LIFE BELOW WATER
2
Research Products
15LIFE ON LAND
0
Research Products
16PEACE, JUSTICE AND STRONG INSTITUTIONS
0
Research Products
17PARTNERSHIPS FOR THE GOALS
0
Research Products
Documents
80
Citations
1682
h-index
22
Documents
84
Citations
1762
Scholarly Output
77
Articles
29
Views / Downloads
154723/24025
Supervised MSc Theses
15
Supervised PhD Theses
5
WoS Citation Count
548
Scopus Citation Count
692
Patents
0
Projects
13
WoS Citations per Publication
7.12
Scopus Citations per Publication
8.99
Open Access Source
46
Supervised Theses
20
| Journal | Count |
|---|---|
| Analyst | 3 |
| 2019 Innovations in Intelligent Systems and Applications Conference, ASYU 2019 | 3 |
| 2020 Medical Technologies Congress (Tiptekno) | 3 |
| ACS Omega | 2 |
| Scientific Reports | 2 |
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77 results
Scholarly Output Search Results
Now showing 1 - 10 of 77
Article Citation - WoS: 2Citation - Scopus: 3Dynamic Fluidic Manipulation in Microfluidic Chips With Dead-End Channels Through Spinning: the Spinochip Technology for Hematocrit Measurement, White Blood Cell Counting and Plasma Separation(Royal Soc Chemistry, 2025) Oksuz, Cemre; Tekin, Hüseyin Cumhur; Bicmen, Can; Tekin, H. CumhurCentrifugation is crucial for size and density-based sample separation, but low-volume or delicate samples suffer from loss and impurity issues during repeated spins. We introduce the "Spinochip", a novel microfluidic system utilizing centrifugal forces for efficient filling of dead-end microfluidic channels. The Spinochip enables versatile fluid manipulation with a single reservoir for both inlet and outlet functions. It expels compressed air, facilitating fluid flow, and offers programmable filling mechanisms based on the hydraulic resistance of microfluidic channels. Compatible with a basic centrifuge, it allows sequential filling, internal mixing, and collection in straight microfluidic channels by simply adjusting the spinning speed, eliminating the need for complex valving. We demonstrated the Spinochip's efficacy in blood testing, where it successfully separated blood components, such as plasma, buffy coat, and red blood cells, from a single drop using centrifugation alone. This system enabled simultaneous hematocrit (R2 >0.99) and total white blood cell (R2 >0.93) quantification within a single microfluidic channel without the need for staining or special reagents. Remarkably, the Spinochip can perform hematocrit measurements on as little as 100 nL of blood, potentially paving the way for less invasive blood analysis. This innovative approach unlocks new possibilities in microfluidics, providing precise fluidic control and centrifugation for sample volumes as small as a few nanoliters.Conference Object Electromechanical Lab-On Platform for Creatinine Analysis Using Automated Elisa Protocols(Chemical and Biological Microsystems Society, 2020) Karakuzu, Betül; Tarım, Ergün Alperay; Öksüz, Cemre; Tekin, Hüseyin CumhurWe present an electromechanical lab-on-a-chip (LOC) platform for the automated serum creatinine detection applying enzyme-linked immunosorbent assay (ELISA) principle. In the platform, antibody covered bar selectively captures the creatinine in the sample and the electromechanical system allows automatic movement between the designed reservoirs containing assay solutions. At the end of the protocol, the absorbance value of the appeared color is measured to determine creatinine concentration in the sample. Since this system allows measuring automatically creatinine levels with minimum time and cost, it can be utilized for point-of-care monitoring of chronic kidney diseases (CKD) for the future. © 2020 CBMS-0001Conference Object Citation - Scopus: 1Magnetic Levitation-Based Adipose Tissue Engineering Using Horizontal Magnet Deployment(IEEE, 2020) Sarıgil, Öykü; Tekin, Hüseyin Cumhur; Anıl İnevi, Müge; Anıl İnevi, Müge; Yılmaz, Esra; Sarıgil, Öykü; Özçelik, Özge; Meşe Özçivici, Gülistan; Meşe, Gülistan; Meşe Özçivici, Gülistan; Tekin, H. CumhurMagnetic levitation is a promising technique for tissue engineering with contact- and label-free approach. Levitation-based biofabrication systems emerge as a simple, rapid and versatile alternative to traditional tissue culture systems, since biofabrication specs can easily be tailored via magnet shape and configuration. This study aims at possible magnetic levitation systems for culture of adipose tissue cells. In this study, we performed two different magnet configurations, vertical and horizontal deployment, in an effort to be utilized in adipose tissue engineering.Master Thesis Fabrication of Microfluidic Devices Via 3d Printer(Izmir Institute of Technology, 2019) Keçili, Seren; Tekin, Hüseyin Cumhur; Bulmuş, VolgaThe purpose of this thesis is to provide easy and rapid prototyping of microfluidic devices using 3D printing technology that overcomes disadvantages of traditional fabrication techniques and also enhanced optical transparency of 3D-printed microfluidic devices fabricated using new bonding strategies. For performance analysis of 3D printer, microfluidic channels and molds having different shape and dimensions were designed and fabricated. After the fabrication process, designed and fabricated channel dimensions were compared. Structures having at least having 50 μm feature were printed successfully. For enhancing transparency of fabricated 3D structures, two different fabrication techniques were developed. In these techniques, 3D structures were bonded on glass substrates with poly (dimethylsiloxane) (PDMS) and Formlabs Clear Resin interlayers. After 3D-printed structures were put on interlayers coated glass slides, they were either remained on coated slides or transferred on new slides. Bonding between 3D structures and glass slides were provided with UV exposure for resin and with elevated temperature for PDMS interlayers. Bonding strength of fabricated channels was investigated for different thicknesses of PDMS and resin interlayers. The bright-field and fluorescence imaging properties of these channels were also analyzed. Proposed fabrication technique showed 2-fold improved bonding strength and comparable bright-field and fluorescence imaging capability with respect to traditional plasma activated PDMS-glass bonding. Furthermore, protein modified glass substrates can be integrated in 3D-printed channels using the presented fabrication technique without disturbing protein functionality. Finally, in order to design a 3D-printed micropump having membranes that can be activated with compressed air, membrane deformation was characterized with different dimension.Conference Object Citation - WoS: 12Citation - Scopus: 17Wearable Respiratory Rate Sensor Technology for Diagnosis of Sleep Apnea(IEEE, 2020) Cinel, Göktürk; Tarım, Ergün Alperay; Tekin, Hüseyin CumhurSleep apnea is a disease that occurs during sleep, which affects the daily life of patients due to the obstruction of the upper respiratory tract and decreases oxygen level in blood and it may even lead to patient death in the later stage. Monitoring the patients regularly has absolute importance to prevent patient disorders caused by sleep apnea. Wearable sensor technologies and patient tracking systems provide diagnosis, treatment, and monitoring of patients, and procure better health services in medical fields. In addition to decreasing the workload of health institutions, remote patient monitoring systems can serve continuous monitoring and determine variable symptoms of the patients. In this paper, we propose a patient monitoring system, which will be used for diagnosis and monitoring of sleep apnea by tracking the respiratory rate of patients with wearable sensor technology. The respiratory rate is detected using either an accelerometer sensor to be placed on the patients' abdomen or a temperature sensor to be placed on their noses. The proposed system offers an increase in the versatility of patient monitoring systems and offers an alternative new technology in sleep apnea diagnosis.Conference Object Citation - Scopus: 2Density-Based Separation of Microparticles Using Magnetic Levitation Technology Integrated on Lensless Holographic Microscopy Platform(Institute of Electrical and Electronics Engineers Inc., 2019) Delikoyun, Kerem; Yaman, Sena; Tekin, Hüseyin CumhurMicroparticle/cell separation is one of the most important applications in the field of biomedical sciences particularly for cell sorting and protein assays. There are variety of different separation technologies introduced in the literature that the main limitations are large amount of sample, expensive chemical use besides of requirement of a labeling procedure (i.e. fluorescent/magnetic labeling), complex machinery, and high operational costs. Magnetic levitation-based separation offers simple, rapid and precise separation of microparticles based on their densities by suspending them in a glass microcapillary between two opposing magnets. Traditionally, magnetic levitation-based microparticle separation and identification procedure is performed by imaging under bulky microscopes composed of fragile and expensive optics and require trained personnel to operate which makes the whole procedure costly, time consuming and prone to human error. Lensless digital inline holographic microscope (LDIHM) eliminates the need for sophisticated optics by replacing simple illumination and recording scheme that can be reduced into few widely-Available and cost-effective components. Thus, inspection procedure is mostly carried out on digitally processing captured holograms so that dependency on optical components and human error is dramatically reduced alongside using cost-effective and handheld device. Here, we introduce a novel hybrid platform that brings the advantages of magnetic levitation system with lensless digital inline holographic microscope for precise separation and identification of microparticles based on their densities. In the platform, it was shown that 1.026 g/mL and 1.090 g/mL microparticles were successfully identified. © 2019 IEEE.Master Thesis Quantitative Phase Analysis in Lensless Digital Inline Holographic Microscopy(01. Izmir Institute of Technology, 2021) Demir, Ali Aslan; Tekin, Hüseyin Cumhur; Varlıklı, CananComputational imaging modalities replace the bulky, complex, and expensive optical components of traditional imaging procedures with numerical reconstruction steps. Digital holographic microscopy is one of the most prominent ones with the possibility of obtaining quantitative phase information by measuring the phase shift change caused by the refractive index of objects. In the lensless digital holographic microscopy system, a pinhole and a light-emitting diode are sufficient to create a holographic pattern on the camera sensor. Here, the optimization of a digital lensless inline holographic microscopy setup was performed to obtain optimal phase value. Also, to retrieve the lost phase information during the recording step, the numerical solution was performed with the single and multi-shot phase retrieval methods. Then, human breast adenocarcinoma (MDA-MB-231) and human myeloid leukemia (U937) cells were analyzed to obtain phase shift, perimeter, and circularity values. These parameters were used to obtain a quantitative differentiation model to replace the traditional labeling or visual confirmation steps with a direct analysis manner. The analysis of respective cells with the classification, object detection, and conditional generative adversarial models can be used directly with pre-trained weights to lessen the computational workloads. With this study, the quantitative analysis with lensless holographic microscopy setup was shown to be a label-free differentiation mechanism to separate cancer cells from monocytes cells which could be used for the early diagnosis of cancer. Also, the proposed method has the potential to be used to identify other cells with links to the diagnosis of different diseases.Conference Object Deep Convolutional Neural Networks for Viability Analysis Directly From Cell Holograms Captured Using Lensless Holographic Microscopy(The Chemical and Biological Microsystems Society (CBMS), 2019) Delikoyun, Kerem; Çine, Ersin; Anıl İnevi, Müge; Özçivici, Engin; Özuysal, Mustafa; Tekin, Hüseyin CumhurCell viability analysis is one of the most widely used protocols in the fields of biomedical sciences. Traditional methods are prone to human error and require high-cost and bulky instrumentations. Lensless digital inline holographic microscopy (LDIHM) offers low-cost and high resolution imaging. However, recorded holograms should be digitally reconstructed to obtain real images, which requires intense computational work. We introduce a deep transfer learning-based cell viability classification method that directly processes the hologram without reconstruction. This new model is only trained once and viability of each cell can be predicted from its hologram. © 2019 CBMS-0001.Article Citation - WoS: 23Citation - Scopus: 30Magnetic Susceptibility-Based Protein Detection Using Magnetic Levitation(American Chemical Society, 2020) Yaman, Sena; Tekin, Hüseyin CumhurMagnetic levitation, which is a magnetic phenomenon of levitating particles suspended in a paramagnetic liquid under a nonuniform magnetic field, is a powerful tool for determining densities and magnetic properties of micro- and nanoparticles. The levitation height of particles in the magnetic field depends on the magnetic susceptibility and density difference between the object and the surrounding liquid. Here, we developed a magnetic susceptibility-based protein detection scheme in a low-cost and miniaturized magnetic levitation setup consisting of two opposing magnets to create a gradient of a magnetic field, a glass capillary channel to retain the sample, and two side mirrors to monitor inside the channel. The method includes the use of polymeric microspheres as mobile assay surfaces and magnetic nanoparticles as labels. The assay was realized by capturing the target protein to the polymer microspheres. Then, magnetic nanoparticles were attached onto the resulting microsphere-protein complex, creating a significant difference in the magnetic properties of polymer microspheres compared to those without protein. The change in the magnetic properties caused a change in the levitation height of the microspheres. The levitation heights and their distribution were then correlated to the amount of target proteins. The method enabled a detection limit of similar to 110 fg/mL biotinylated bovine serum albumin in serum. With the sandwich immunoassay developed for mouse immunoglobulin G, detection limits of 1.5 ng/mL and >10 ng/mL were achieved in buffer and serum, respectively. This approach sensed the minute changes in the volume magnetic susceptibility of the microspheres with a resolution of 4.2 x 10(-8) per 1 mu m levitation height change.Article Citation - WoS: 22Citation - Scopus: 24Scaffold-Free Biofabrication of Adipocyte Structures With Magnetic Levitation(John Wiley and Sons Inc., 2021) Sarıgil, Öykü; Yalçın Özuysal, Özden; Anıl İnevi, Müge; Meşe Özçivici, Gülistan; Fıratlıgil Yıldırır, Burcu; Fıratlıgil Yıldırır, Burcu; Ünal, Yağmur Ceren; Ünal, Yağmur Ceren; Yalçın Özuysal, Özden; Özçivici, Engin; Meşe, Gülistan; Sarıgil, Öykü; Özçivici, Engin; Anıl İnevi, Müge; Meşe Özçivici, GülistanTissue engineering research aims to repair the form and/or function of impaired tissues. Tissue engineering studies mostly rely on scaffold-based techniques. However, these techniques have certain challenges, such as the selection of proper scaffold material, including mechanical properties, sterilization, and fabrication processes. As an alternative, we propose a novel scaffold-free adipose tissue biofabrication technique based on magnetic levitation. In this study, a label-free magnetic levitation technique was used to form three-dimensional (3D) scaffold-free adipocyte structures with various fabrication strategies in a microcapillary-based setup. Adipogenic-differentiated 7F2 cells and growth D1 ORL UVA stem cells were used as model cells. The morphological properties of the 3D structures of single and cocultured cells were analyzed. The developed procedure leads to the formation of different patterns of single and cocultured adipocytes without a scaffold. Our results indicated that adipocytes formed loose structures while growth cells were tightly packed during 3D culture in the magnetic levitation platform. This system has potential for ex vivo modeling of adipose tissue for drug testing and transplantation applications for cell therapy in soft tissue damage. Also, it will be possible to extend this technique to other cell and tissue types.