Büyüköz, Melda

Profile URL
https://hdl.handle.net/11147/16346
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Email Address
Main Affiliation
03.02. Department of Chemical Engineering
Status
Former Staff
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ORCID ID
Scopus Author ID
Turkish CoHE Profile ID
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WoS Researcher ID

Sustainable Development Goals

NO POVERTY1
NO POVERTY
0
Research Products
ZERO HUNGER2
ZERO HUNGER
0
Research Products
GOOD HEALTH AND WELL-BEING3
GOOD HEALTH AND WELL-BEING
0
Research Products
QUALITY EDUCATION4
QUALITY EDUCATION
0
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GENDER EQUALITY5
GENDER EQUALITY
0
Research Products
CLEAN WATER AND SANITATION6
CLEAN WATER AND SANITATION
0
Research Products
AFFORDABLE AND CLEAN ENERGY7
AFFORDABLE AND CLEAN ENERGY
1
Research Products
DECENT WORK AND ECONOMIC GROWTH8
DECENT WORK AND ECONOMIC GROWTH
0
Research Products
INDUSTRY, INNOVATION AND INFRASTRUCTURE9
INDUSTRY, INNOVATION AND INFRASTRUCTURE
2
Research Products
REDUCED INEQUALITIES10
REDUCED INEQUALITIES
0
Research Products
SUSTAINABLE CITIES AND COMMUNITIES11
SUSTAINABLE CITIES AND COMMUNITIES
0
Research Products
RESPONSIBLE CONSUMPTION AND PRODUCTION12
RESPONSIBLE CONSUMPTION AND PRODUCTION
1
Research Products
CLIMATE ACTION13
CLIMATE ACTION
1
Research Products
LIFE BELOW WATER14
LIFE BELOW WATER
0
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LIFE ON LAND15
LIFE ON LAND
0
Research Products
PEACE, JUSTICE AND STRONG INSTITUTIONS16
PEACE, JUSTICE AND STRONG INSTITUTIONS
0
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PARTNERSHIPS FOR THE GOALS17
PARTNERSHIPS FOR THE GOALS
0
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This researcher does not have a Scopus ID.
This researcher does not have a WoS ID.
Scholarly Output

5

Articles

4

Views / Downloads

4500/2081

Supervised MSc Theses

0

Supervised PhD Theses

1

WoS Citation Count

73

Scopus Citation Count

84

Patents

0

Projects

2

WoS Citations per Publication

14.60

Scopus Citations per Publication

16.80

Open Access Source

5

Supervised Theses

1

JournalCount
Acta Biomaterialia1
Biointerface Research in Applied Chemistry1
Celal Bayar Üniversitesi Fen Bilimleri Dergisi1
Journal of Tissue Engineering and Regenerative Medicine1
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2014 - 20185

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Scholarly Output Search Results

Now showing 1 - 5 of 5
  • Article
    Citation - WoS: 6
    Antioxidant, Antimicrobial and Cytotoxic Activities of Extracts From Some Selected Mediterranean Shrub Species (maquis)
    (Biointerface Research Applied Chemistry, 2016) Bayraktar, Oğuz; Altıok, Evren; Yılmazer, Özgür; Rusçuklu, Dane; Büyüköz, Melda
    In this study in vitro antioxidant, antimicrobial and cytotoxic activities of ethanol extracts of some plants from Urla region in Turkey were investigated. Plant material samples of Pistacia lentiscus, Vitex agnus-castus, Cistus creticus and Nerium oleander were collected in October, November and December. The harvesting time significantly affected their antioxidant, antimicrobial and cytotoxic activities of these plant extracts. The highest biological activities in terms of antioxidant, antimicrobial and cytotoxic activities were observed for the leaf extract of C. creticus. The applied doses of leaf extracts of C. creticus, P. lentiscus, and N. oleander resulted in higher Bax and GAPDH expressions than those for control cells. These plant extracts may trigger apoptosis and may be a promising natural source for prostate cancer treatment.
  • Article
    Jelatin Doku İskelesinin Mekanik Özellikleri Üzerine Gözenek Oluşturucu Ajanın Boyutu ve Bağlantı Süresinin Etkileri
    (Manisa Celâl Bayar Üniversitesi, 2015) Büyüköz, Melda; Alsoy Altınkaya, Sacide
    Bu çalışmada, makrogözenekli ve gözenekleri birbirleri ile bağlantılı olarak kalıplama, ısı etkili faz ayrımı ve tanecik uzaklaştırma yöntemleri ile üretilen jelatin bazlı doku iskelelerinde gözenek oluşturucu ajan olan parafin kürelerin boyutu ve bağlantı sürelerinin doku iskelelerinin mekanik özellikleri üzerine olan etkileri incelenmiştir. Sünger formlu ve açık hücreli yapıda olan doku iskelelerinin mekanik özellikleri sıkıştırma testi ile ölçülmüştür. Yapılan ölçümler parafin küre çapındaki artışın doku iskelelerinin sıkıştırma katsayısını istatistiksel olarak anlamlı bir şekilde etkilemediğini göstermiştir. Ancak, parafin kürelerin maruz bırakıldığı ısı etkileşim süresinin 100 dakikadan 400 dakikaya arttırılmasının 250-425 µm çapındaki parafin kürelerle hazırlanan doku iskelesinin elastisite değerini arttırdığı belirlenmiştir (p<0.05). Ayrıca, 425-600 µm çapındaki parafin kürelerle hazırlanan doku iskelelerinde, ısı etkileşim süresi 100 dakikadan 400 dakikaya arttırıldığında sıkıştırma katsayısı anlamlı bir şekilde azalmıştır (p<0.05). Elde edilen sonuçlar, bu tür doku iskelelerinde parafin kürelerin boyutundan çok bağlantı süresinin mekanik özellikler üzerinde etkili olduğunu göstermiştir
  • Article
    Citation - WoS: 27
    Citation - Scopus: 37
    Nanofibrous Gelatine Scaffolds Integrated With Nerve Growth Factor-Loaded Alginate Microspheres for Brain Tissue Engineering
    (John Wiley and Sons Inc., 2018) Büyüköz, Melda; Erdal, Esra; Alsoy Altınkaya, Sacide
    Neural regeneration research is designed in part to develop strategies for therapy after nerve damage due to injury or disease. In this study, a new gelatine-based biomimetic scaffold was fabricated for brain tissue engineering applications. A technique combining thermally induced phase separation and porogen leaching was used to create interconnected macropores and nanofibrous structure. To promote tissue regeneration processes, the scaffolds were integrated with nerve growth factor (NGF)-loaded alginate microspheres. The results showed that nanofibrous matrix could only be obtained when gelatine concentration was at least 7.5% (w/v). The scaffold with a modulus value (1.2 kPa) similar to that of brain tissue (0.5–1 kPa) was obtained by optimizing the heat treatment time, macropore size and gelatine concentration. The encapsulation efficiencies of NGF into 0.1% and 1% alginate microspheres were 85% and 100%, respectively. The release rate of NGF from the microspheres was controlled by the alginate concentration and the poly(L-lysine) coating. The immobilization of the microspheres in the scaffold reduced burst release and significantly extended the release period. The nanofibrous architecture and controlled release of NGF from the microspheres induced neurite extension of PC12 cells, demonstrating that the released NGF was in an active form. The results suggest that the scaffolds prepared in this study may have potential applications in brain tissue engineering due to topologic and mechanical properties similar to brain tissue and pore structure suitable for cell growth and differentiation.
  • Article
    Citation - WoS: 40
    Citation - Scopus: 47
    Gelatin-Based 3d Conduits for Transdifferentiation of Mesenchymal Stem Cells Into Schwann Cell-Like Phenotypes
    (Elsevier Ltd., 2017) Uz, Metin; Büyüköz, Melda; Sharma, Anup D.; Sakaguchi, Donald S.; Alsoy Altınkaya, Sacide; Mallapragada, Surya K.
    In this study, gelatin-based 3D conduits with three different microstructures (nanofibrous, macroporous and ladder-like) were fabricated for the first time via combined molding and thermally induced phase separation (TIPS) technique for peripheral nerve regeneration. The effects of conduit microstructure and mechanical properties on the transdifferentiation of bone marrow-derived mesenchymal stem cells (MSCs) into Schwann cell (SC) like phenotypes were examined to help facilitate neuroregeneration and understand material-cell interfaces. Results indicated that 3D macroporous and ladder-like structures enhanced MSC attachment, proliferation and spreading, creating interconnected cellular networks with large numbers of viable cells compared to nanofibrous and 2D-tissue culture plate counterparts. 3D-ladder-like conduit structure with complex modulus of ∼0.4 × 106 Pa and pore size of ∼150 μm provided the most favorable microenvironment for MSC transdifferentiation leading to ∼85% immunolabeling of all SC markers. On the other hand, the macroporous conduits with complex modulus of ∼4 × 106 Pa and pore size of ∼100 μm showed slightly lower (∼65% for p75, ∼75% for S100 and ∼85% for S100β markers) immunolabeling. Transdifferentiated MSCs within 3D-ladder-like conduits secreted significant amounts (∼2.5 pg/mL NGF and ∼0.7 pg/mL GDNF per cell) of neurotrophic factors, while MSCs in macroporous conduits released slightly lower (∼1.5 pg/mL NGF and 0.7 pg/mL GDNF per cell) levels. PC12 cells displayed enhanced neurite outgrowth in media conditioned by conduits with transdifferentiated MSCs. Overall, conduits with macroporous and ladder-like 3D structures are promising platforms in transdifferentiation of MSCs for neuroregeneration and should be further tested in vivo. Statement of Significance This manuscript focuses on the effect of microstructure and mechanical properties of gelatin-based 3D conduits on the transdifferentiation of mesenchymal stem cells to Schwann cell-like phenotypes. This work builds on our recently accepted manuscript in Acta Biomaterialia focused on multifunctional 2D films, and focuses on 3D microstructured conduits designed to overcome limitations of current strategies to facilitate peripheral nerve regeneration. The comparison between conduits fabricated with nanofibrous, macroporous and ladder-like microstructures showed that the ladder-like conduits showed the most favorable environment for MSC transdifferentiation to Schwann-cell like phenotypes, as seen by both immunolabeling as well as secretion of neurotrophic factors. This work demonstrates the importance of controlling the 3D microstructure to facilitate tissue engineering strategies involving stem cells that can serve as promising approaches for peripheral nerve regeneration.
  • Doctoral Thesis
    Preparation and Characterization of Polymeric Scaffolds for Nerve Tissue Engineering Applications
    (Izmir Institute of Technology, 2014) Büyüköz, Melda; Alsoy Altınkaya, Sacide; Erdal, Şerife Esra
    The major goal in tissue engineering is to develop three-dimensional biomimetic scaffolds which can provide an optimal environment for cell adhesion, proliferation, differentiation and guide new tissue formation. In this study macroporous, nanofibrous gelatin scaffolds in the form of a disc and channeled conduit were prepared for nerve tissue engineering applications. Alginate microspheres have been integrated into the scaffolds to deliver nerve growth factor (NGF) to differentiate PC12 cells. Methods combining thermally induced phase separation technique with porogen leaching and injection molding were used to manifacture disc shaped and channeled nanofibrous scaffolds, respectively. Microcarriers loaded with NGF were fabricated by water-in-oil emulsification technique and attached in the scaffold by chemical crosslinking with carbodiimide reaction. The relationship among processing parameter, porosity, pore size, interpore connectivity and the mechanical properties were investigated. In addition release kinetics of NGF from the particles were determined and viability, proliferation and differentiation of PC12 cells in the scaffolds were evaluated. The fiber sizes of nanofibrous scaffolds were found similar to the size of natural collagen fiber bundles. In nanofibrous scaffolds, the dimensional stability and in vitro degredation rates improved when compared to solid walled scaffolds. The release rate of NGF from the particles was controlled by the alginate concentration and poly(L-lysine) coating. Integrating NGF into the nanofibrous gelatin scaffold in encapsulated form reduced amount of NGF and time required for the differentiation of PC12 compared to free NGF directly added to the cells.