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Kuru, Gülten
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01. Izmir Institute of Technology
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External
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Sustainable Development Goals
1NO POVERTY
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2ZERO HUNGER
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3GOOD HEALTH AND WELL-BEING
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4QUALITY EDUCATION
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5GENDER EQUALITY
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6CLEAN WATER AND SANITATION
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7AFFORDABLE AND CLEAN ENERGY
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8DECENT WORK AND ECONOMIC GROWTH
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9INDUSTRY, INNOVATION AND INFRASTRUCTURE
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10REDUCED INEQUALITIES
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11SUSTAINABLE CITIES AND COMMUNITIES
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12RESPONSIBLE CONSUMPTION AND PRODUCTION
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13CLIMATE ACTION
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14LIFE BELOW WATER
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15LIFE ON LAND
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16PEACE, JUSTICE AND STRONG INSTITUTIONS
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17PARTNERSHIPS FOR THE GOALS
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Scholarly Output
4
Articles
2
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5966/1558
Supervised MSc Theses
1
Supervised PhD Theses
1
WoS Citation Count
16
Scopus Citation Count
15
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0
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0
WoS Citations per Publication
4.00
Scopus Citations per Publication
3.75
Open Access Source
2
Supervised Theses
2
| Journal | Count |
|---|---|
| Molecules | 1 |
| Phytochemistry Reviews | 1 |
Current Page: 1 / 1
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4 results
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Now showing 1 - 4 of 4
Article Citation - WoS: 12Citation - Scopus: 12New Cardenolides From Biotransformation of Gitoxigenin by the Endophytic Fungus Alternaria Eureka 1e1bl1: Characterization and Cytotoxic Activities(MDPI, 2021) Kuru, Gülten; Bedir, Erdal; Küçüksolak, Melis; Kuru, Gülten; Küçüksolak, Melis; Yusufoğlu, Hasan; 03.01. Department of Bioengineering; 01. Izmir Institute of Technology; 03. Faculty of EngineeringMicrobial biotransformation is an important tool in drug discovery and for metabolism studies. To expand our bioactive natural product library via modification and to identify possible mammalian metabolites, a cytotoxic cardenolide (gitoxigenin) was biotransformed using the endophytic fungus Alternaria eureka 1E1BL1. Initially, oleandrin was isolated from the dried leaves of Nerium oleander L. and subjected to an acid-catalysed hydrolysis to obtain the substrate gitoxigenin (yield; similar to 25%). After 21 days of incubation, five new cardenolides 1, 3, 4, 6, and 8 and three previously- identified compounds 2, 5 and 7 were isolated using chromatographic methods. Structural elucidations were accomplished through 1D/2D NMR, HR-ESI-MS and FT-IR analysis. A. eureka catalyzed oxygenation, oxidation, epimerization and dimethyl acetal formation reactions on the substrate. Cytotoxicity of the metabolites were evaluated using MTT cell viability method, whereas doxorubicin and oleandrin were used as positive controls. Biotransformation products displayed less cytotoxicity than the substrate. The new metabolite 8 exhibited the highest activity with IC50 values of 8.25, 1.95 and 3.4 mu M against A549, PANC-1 and MIA PaCa-2 cells, respectively, without causing toxicity on healthy cell lines (MRC-5 and HEK-293) up to concentration of 10 mu M. Our results suggest that A. eureka is an effective biocatalyst for modifying cardenolide-type secondary metabolites.Doctoral Thesis Effects of Telomerase Activators on Monoclonal Antibody-Producing Cell Lines and Stem Cells, and Their Utilization in Industrial Productions(01. Izmir Institute of Technology, 2024) Bedir, Erdal; Kuru, Gülten; Bedir, Erdal; 03.01. Department of Bioengineering; 01. Izmir Institute of Technology; 03. Faculty of EngineeringAging is a physiological and multifactorial biological process of functional decline in any living organism. Telomere shortening, high levels of reactive oxygen species (ROS), and cellular senescence are the primary physiological changes that accompany aging. While there has been an increase in human life expectancy in recent decades, there has not been a concomitant increase in healthy aging. Degenerative diseases, including musculoskeletal disorders such as osteoporosis and osteoarthritis, have been found to be directly linked to aging. Age-related degenerative diseases are devastating diseases that cause millions of deaths worldwide each year and place an economic and psychological burden on society. Due to the ever-increasing number of patients, there is a huge demand for novel therapeutic approaches to treat degenerative diseases. Two main approaches are at the forefront of technology for the treatment of degenerative diseases: stem cell transplantation (regenerative medicine) and monoclonal antibody-based therapy. Indeed, it is well known that there is a strong correlation between disease pathology and telomeres. In fact, the possible therapeutic effects of telomerase activation have been evaluated in diverse backgrounds to cure and prevent various diseases. Within the scope of this thesis, we aim to investigate the effects of telomerase activator novel molecules from Astragalus sp., obtained in our previous studies by biotransformation of cycloastragenol (CG) via the plant's endophytic fungi on the health span/lifespan of mesenchymal stem cells (MSCs) during in vitro expansion and their osteogenic differentiation. Additionally, the efficacy of these compounds was investigated in the monoclonal antibody (mAb) production process in terms of mAb productivity. Based on the outcomes of the study, novel telomerase activators deriving from natural resources of our country have significant potential in stem cell research, thus regenerative medicine, since promising results were obtained for the clinical use of these novel molecules. Our data also suggest that molecules simultaneously promote osteogenic differentiation and telomerase activation.Article Citation - WoS: 4Citation - Scopus: 3Is Telomerase a Hidden Player? Therapeutic Potential of Natural Telomerase Activators Against Age-Related Diseases(Springer, 2022) Kuru, Gülten; Üner, Göklem; Bedir, Erdal; Üner, Göklem; Kuru, Gülten; 03.01. Department of Bioengineering; 01. Izmir Institute of Technology; 03. Faculty of EngineeringThere is a huge demand for novel treatment and/or prevention approaches for age-related diseases, which reduce life quality and one of the main reasons for death worldwide. Many age-related diseases were found to be associated with dysfunctional telomeres, which accelerate aging process due to the decrease in repair potential of tissues. An enzyme called telomerase is mainly responsible for keeping telomeres healthful. In the last two decades, the progress in the field, including in vitro studies, preclinical data, and human trials, demonstrated that telomerase and related genes might be powerful targets for the treatment of those diseases. Considering telomerase reactivation as a treatment strategy in age-related degenerative diseases, telomerase activators obtained from natural products stand out as promising agents. Although various research showed that those activators have protective/therapeutic activity against age-related diseases, the role of telomerase activation is often neglected in studies. In this context, we focused on the natural products as telomerase activator and their activities on age-related diseases, specifically neurodegenerative, cardiovascular, and osteodegenerative disorders, in which telomere dysfunction plays a causal role. Thus, this review aims to draw attention to the possibility of telomerase activation in therapy, in which some well-known natural products such as telomerase activators might play a role.Master Thesis Investigations on the Effects of Carbon Sources and Ph on Exo-Polygalacturonase Productions by Aspergillus Sojae(Izmir Institute of Technology, 2013) Kuru, Gülten; Büyükkileci, Ali Oğuz; Kuru, Gülten; Büyükkileci, Ali Oğuz; 01. Izmir Institute of Technology; 03.08. Department of Food Engineering; 03. Faculty of EngineeringPectinases are a group of enzymes that are responsible for degredation of pectic substances. Pectinases are produced by many organisms including plants, bacteria and fungi. The aim of this study was to investigate the effect of different carbon sources and pH on pectinase production by a group of flamentous fungi. For this purpose, Aspergillus niger, Rhizopus oryzae ATCC 4858, Aspergillus sojae ATCC 2035 (A. sojae WT) and its mutant type Aspegillus sojae MT (A. sojae MT) grown in orange peel containing media were evaluated in terms of exo-polygalacturonase (exo-PG), pectin lyase (PL), pectin methyl esterase (PME), and also xylanase and cellulase activities. A. sojae WT and A. sojae MT were found to be as exo-PG producers. Maximum exo-PG activities were 47.84 U/ml and 108.02 U/ml, respectively. Exo-PG production by A. sojae WT and A. sojae MT was evaluated using the medium containing glucose, pectin, pectin/glucose, pectin/fructose and pectin/sucrose as substrate. Additional supply of glucose to the pectin media was found to be more effective than fructose or sucrose. The fermentations in which pH was not maintained, the use of glucose, pectin and pectin/glucose resulted in superior enzyme activities compared to ones obtained with other media compositions.