This item is non-discoverable
Kara, Aylin
Loading...
Profile URL
Name Variants
Kara Özenler, Aylin
Ozenler, Aylin Kara
Özenler, Aylin Kara
Kara, Ozenler, A.
Ozenler, Aylin Kara
Özenler, Aylin Kara
Kara, Ozenler, A.
Job Title
Email Address
Main Affiliation
03.01. Department of Bioengineering
Status
External
Website
ORCID ID
Scopus Author ID
Turkish CoHE Profile ID
Google Scholar ID
WoS Researcher ID
Sustainable Development Goals
1NO POVERTY
0
Research Products
2ZERO HUNGER
0
Research Products
3GOOD HEALTH AND WELL-BEING
4
Research Products
4QUALITY EDUCATION
0
Research Products
5GENDER EQUALITY
0
Research Products
6CLEAN WATER AND SANITATION
0
Research Products
7AFFORDABLE AND CLEAN ENERGY
1
Research Products
8DECENT WORK AND ECONOMIC GROWTH
0
Research Products
9INDUSTRY, INNOVATION AND INFRASTRUCTURE
4
Research Products
10REDUCED INEQUALITIES
0
Research Products
11SUSTAINABLE CITIES AND COMMUNITIES
0
Research Products
12RESPONSIBLE CONSUMPTION AND PRODUCTION
0
Research Products
13CLIMATE ACTION
0
Research Products
14LIFE BELOW WATER
0
Research Products
15LIFE ON LAND
0
Research Products
16PEACE, JUSTICE AND STRONG INSTITUTIONS
0
Research Products
17PARTNERSHIPS FOR THE GOALS
0
Research Products
This researcher does not have a Scopus ID.
WoS data could not be loaded because of an error. Please refresh the page or try again later.
Publication Collaboration
| Affiliation Name | Count |
|---|---|
| Izmir Institute of Technology | 11 |
| Dokuz Eylül University | 7 |
| Friedrich-Alexander-Universität Erlangen-Nürnberg | 3 |
| Izmir University | 2 |
| University Medical Center Utrecht | 2 |
1 / 5
Data obtained from OpenAlex
Scholarly Output
11
Articles
10
Views / Downloads
30963/5355
Supervised MSc Theses
0
Supervised PhD Theses
1
WoS Citation Count
212
Scopus Citation Count
251
Patents
0
Projects
0
WoS Citations per Publication
19.27
Scopus Citations per Publication
22.82
Open Access Source
6
Supervised Theses
1
| Journal | Count |
|---|---|
| Journal of Biomaterials Applications | 3 |
| Biofabrication | 2 |
| Dokuz Eylül Üniversitesi Mühendislik Fakültesi Fen ve Mühendislik Dergisi | 1 |
| Materialia | 1 |
| Materials Today Bio | 1 |
Current Page: 1 / 2
Scopus Quartile Distribution
Competency Cloud
11 results
Scholarly Output Search Results
Now showing 1 - 10 of 11
Article Citation - WoS: 25Citation - Scopus: 30Fabrication of 3d Printed Poly(lactic Acid) Strut and Wet-Electrospun Cellulose Nano Fiber Reinforced Chitosan-Collagen Hydrogel Composite Scaffolds for Meniscus Tissue Engineering(SAGE Publications, 2022) Güneş, Oylum Çolpankan; Kara, Aylin; Baysan, Gizem; Hüsemoğlu, Reşit Buğra; Akokay, Pınar; Ziylan Albayrak, Aylin; Ergür, Bekir Uğur; Havitçioğlu, Hasan; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyThe main goal of the study was to produce chitosan-collagen hydrogel composite scaffolds consisting of 3D printed poly(lactic acid) (PLA) strut and nanofibrous cellulose for meniscus cartilage tissue engineering. For this purpose, first PLA strut containing microchannels was incorporated into cellulose nanofibers and then they were embedded into chitosan-collagen matrix to obtain micro- and nano-sized topographical features for better cellular activities as well as mechanical properties. All the hydrogel composite scaffolds produced by using three different concentrations of genipin (0.1, 0.3, and 0.5%) had an interconnected microporous structure with a swelling ratio of about 400% and water content values between 77 and 83% which is similar to native cartilage extracellular matrix. The compressive strength of all the hydrogel composite scaffolds was found to be similar (∼32 kPa) and suitable for cartilage tissue engineering applications. Besides, the hydrogel composite scaffold comprising 0.3% (w/v) genipin had the highest tan δ value (0.044) at a frequency of 1 Hz which is around the walking frequency of a person. According to the in vitro analysis, this hydrogel composite scaffold did not show any cytotoxic effect on the rabbit mesenchymal stem cells and enabled cells to attach, proliferate and also migrate through the inner area of the scaffold. In conclusion, the produced hydrogel composite scaffold holds great promise for meniscus tissue engineering.Article Citation - WoS: 14Citation - Scopus: 14Development of Biological Meniscus Scaffold: Decellularization Method and Recellularization With Meniscal Cell Population Derived From Mesenchymal Stem Cells(SAGE Publications, 2021) Kara, Aylin; Koçtürk, Semra; Bilici, Gökçen; Havıtçıoğlu, Hasan; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyTissue engineering approaches which include a combination of cells and scaffold materials provide an alternative treatment for meniscus regeneration. Decellularization and recellularization techniques are potential treatment options for transplantation. Maintenance of the ultrastructure composition of the extracellular matrix and repopulation with cells are important factors in constructing a biological scaffold and eliminating immunological reactions. The aim of the study is to develop a method to obtain biological functional meniscus scaffolds for meniscus regeneration. For this purpose, meniscus tissue was decellularized by our modified method, a combination of physical, chemical, and enzymatic methods and then recellularized with a meniscal cell population composed of fibroblasts, chondrocytes and fibrochondrocytes that obtained from mesenchymal stem cells. Decellularized and recellularized meniscus scaffolds were analysed biochemically, biomechanically and histologically. Our results revealed that cellular components of the meniscus were successfully removed by preserving collagen and GAG structures without any significant loss in biomechanical properties. Recellularization results showed that the meniscal cells were localized in the empty lacuna on the decellularized meniscus, and also well distributed and proliferated consistently during the cell culture period (p < 0.05). Furthermore, a high amount of DNA, collagen, and GAG contents (p < 0.05) were obtained with the meniscal cell population in recellularized meniscus tissue. The study demonstrates that our decellularization and recellularization methods were effective to develop a biological functional meniscus scaffold and can mimic the meniscus tissue with structural and biochemical features. We predict that the obtained biological meniscus scaffolds may provide avoidance of adverse immune reactions and an appropriate microenvironment for allogeneic or xenogeneic recipients in the transplantation process. Therefore, as a promising candidate, the obtained biological meniscus scaffolds might be verified with a transplantation experiment.Article Citation - WoS: 54Citation - Scopus: 64Bioactive Fish Scale Incorporated Chitosan Biocomposite Scaffolds for Bone Tissue Engineering(Elsevier Ltd., 2019) Kara, Aylin; Tıhmınlıoğlu, Funda; Tamburacı, Sedef; Kara, Aylin; Tıhmınlıoğlu, Funda; Havıtçıoğlu, Hasan; 03.02. Department of Chemical Engineering; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyRecently, biologically active natural macromolecules have come into prominence to be used as potential materials in scaffold design due to their unique characteristics which can mimic the human tissue structure with their physical and chemical similarity. Among them, fish scale (FS) is a biologically active material with its structural similarity to bone tissue due to including type I collagen and hydroxyapatite and also have distinctive collagen arrangement. In the present study, it is aimed to design a novel composite scaffold with FS incorporation into chitosan (CH) matrix for bone tissue regeneration. Therefore, two biological macromolecules, fish scale and chitosan, were combined to produce bio-composite scaffold. First, FS were decellularized with the chemical method and disrupted physically as microparticles (100 in), followed by dispersal in CH with ultrasonic homogenisation, CH/FS scaffolds were fabricated by lyophilization technique. Scaffolds were characterized physically, chemically, mechanically, and morphologically. SEM and porosity results showed that CH/FS scaffolds have uniform pore structure showing high porosity. Mechanical properties and degradation rate are enhanced with increasing FS content. In vitro cytotoxicity, proliferation and osteogenic activity of the scaffolds were evaluated with SaOS-2 cell line. CH/FS scaffolds did not show any cytotoxicity effect and the cells were gradually proliferated during culture period. Cell viability results showed that, FS microparticles had a proliferative effect on SaOS-2 cells when compared to control group. ALP activity and biomineralization studies indicated that FS micro particle reinforcement increased osteogenic activity during culture period. As a biological macromolecule with unique characteristics, FS was found as cytocompatible and provided promising effects as reinforcement agents for polymeric scaffolds. In conclusion, fabricated CH/FS bio-composites showed potential for bone tissue engineering applications. (C) 2019 Elsevier B.V. All rights reserved.Article Citation - WoS: 14Citation - Scopus: 17Biocompatibility of Silicon Nitride Produced Via Partial Sintering & Tape Casting(Elsevier Ltd., 2021) Çeçen, Berivan; Kara, Aylin; Topateş, Gülsüm; Kara, Aylin; Akbulut, Serdar Onat; Havıtçıoğlu, Hasan; Kozacı, Leyla Didem; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyThe biocompatibility of silicon nitride ceramics was proven by several studies however this study is apart from the literature in the manner of production routes that are tape casting and partial sintering. We report the tape casting route was chosen and a porous structure was obtained by partial sintering technique. Tape casting brought a smooth surface to the samples. Density and pore size distribution analysis showed that the scaffolds have low density because of the porous structure. XRD and SEM analyses were carried out to reveal the phase and microstructural characteristics of porous ceramic samples. Static contact angle measurement was done for the characterization of the wettability of the scaffolds. It revealed that the surface of the scaffolds was highly hydrophilic which is a desirable characteristic for the protein and cell adhesion. The mechanical characteristics of the scaffolds were analyzed by compression tests. Human osteosarcoma cells were used for in vitro studies. Cell-proliferation and cytotoxicity were analyzed by WST-1 and LDH, respectively. The osteoblastic behavior of the cells on the surface of the scaffolds was identified by alkaline phosphatase activity. BCA analysis was used for total protein content. The BCA and ALP results showed an increasing trend which is directly correlated with cell proliferation. Cells on the surface of the silicon nitride scaffolds were visualized by SEM and fluorescence microscopy where the images supported the in vitro analysis. Therefore, porous silicon nitride scaffolds fabricated via tape casting and partial sintering were biocompatible and they are possible candidates as bone substitute elements. © 2020 Elsevier Ltd and Techna Group S.r.l.Article Citation - WoS: 5Citation - Scopus: 9Using Loofah Reinforced Chitosan-Collagen Hydrogel Based Scaffolds In-Vitro and In-Vivo; Healing in Cartilage Tissue Defects(Elsevier B.V., 2023) Baysan, G.; Kara, Aylin; Gunes, O.C.; Turemis, C.; Akokay, Yilmaz, P.; Husemoglu, R.B.; Kara, Ozenler, A.; Perpelek, M.; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyThe herein article aims to report a new scaffold design as a loofah-reinforced chitosan-collagen hydrogel composite scaffold with three different cross-linker concentrations (0.1, 0.3, and 0.5 wt. /v%). From the analyses, the scaffold crosslinked with 0.5% genipin; collagen-chitosan hydrogel scaffold reinforced with loofah (L-CCol5) was found to be suitable for further in vitro and in vivo studies due to its interconnected porous structure, water content (∼ 97%) and tan delta (0.221 at 1 Hz) values comparable to that of cartilage tissue. In vitro analyses depicted that the L-CCol5 scaffold supported rabbit mesenchymal stem cells (rMSCs) adhesion and proliferation with its non-cytotoxic feature. Moreover, in vivo cartilage healing studies were performed using New Zealand male rabbits in three groups: empty control, cell-free scaffold, and rMSCs-laden scaffold. The elastic moduli of these three groups were 0.69, 0.90, and 1.18 MPa, respectively. Besides, microcomputer tomography (MicroCT) scannings supported the in vivo biomechanical analyses as cell-laden scaffolds showed better osteochondral healing. It can be concluded that the L-CCol5 scaffold could be a promising construct in osteochondral tissue engineering applications. The findings revealed that osteochondral remodeling precedes articular cartilage, providing insight into tailored therapeutic approaches, disease progress, and treatment consequences. © 2023 Acta Materialia Inc.Article Citation - WoS: 12Citation - Scopus: 17Fish Scale/Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Nanofibrous Composite Scaffolds for Bone Regeneration(SAGE Publications, 2020) Kara, Aylin; Kara, Aylin; Güneş, Oylum C.; Albayrak, Aylin Z.; Bilici, Gökçen; Erbil, Güven; Havitcioğlu, Hasan; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyThe aim of this study was to produce three-dimensional, nanofibrous fish scale/poly(3-hydroxybutyrate-co-3-hydroxyvalerate) composite scaffolds as bone filling materials. This is the first report wherein fish scales were used within a nanofibrous matrix for bone regeneration. Composite scaffolds with a cotton wool-like structure (fiber diameter: 560 +/- 64 nm; porosity: 82%) were obtained by incorporating chopped fish scales into wet-electrospun poly(3-hydroxybutyrate-co-3-hydroxyvalerate) nanofibers and freeze-drying. The addition of the fish scales improved the mechanical properties, biomineralization tendency, cell viability, alkaline phosphatase activity, and type I collagen production. Consequently, produced composite scaffolds would be regarded to have the therapeutic capacity in bone tissue damages.Article Citation - WoS: 14Citation - Scopus: 163D Bioprinting of mouse pre-osteoblasts and human MSCs using bioinks consisting of gelatin and decellularized bone particles(Iop Publishing Ltd, 2024) Kara, Aylin; Tıhmınlıoğlu, Funda; Akkineni, Ashwini Rahul; Tihminlioglu, Funda; Gelinsky, Michael; Boccaccini, Aldo R.; 03.02. Department of Chemical Engineering; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyOne of the key challenges in biofabrication applications is to obtain bioinks that provide a balance between printability, shape fidelity, cell viability, and tissue maturation. Decellularization methods allow the extraction of natural extracellular matrix, preserving tissue-specific matrix proteins. However, the critical challenge in bone decellularization is to preserve both organic (collagen, proteoglycans) and inorganic components (hydroxyapatite) to maintain the natural composition and functionality of bone. Besides, there is a need to investigate the effects of decellularized bone (DB) particles as a tissue-based additive in bioink formulation to develop functional bioinks. Here we evaluated the effect of incorporating DB particles of different sizes (<= 45 and <= 100 mu m) and concentrations (1%, 5%, 10% (wt %)) into bioink formulations containing gelatin (GEL) and pre-osteoblasts (MC3T3-E1) or human mesenchymal stem cells (hTERT-MSCs). In addition, we propose a minimalistic bioink formulation using GEL, DB particles and cells with an easy preparation process resulting in a high cell viability. The printability properties of the inks were evaluated. Additionally, rheological properties were determined with shear thinning and thixotropy tests. The bioprinted constructs were cultured for 28 days. The viability, proliferation, and osteogenic differentiation capacity of cells were evaluated using biochemical assays and fluorescence microscopy. The incorporation of DB particles enhanced cell proliferation and osteogenic differentiation capacity which might be due to the natural collagen and hydroxyapatite content of DB particles. Alkaline phosphatase activity is increased significantly by using DB particles, notably, without an osteogenic induction of the cells. Moreover, fluorescence images display pronounced cell-material interaction and cell attachment inside the constructs. With these promising results, the present minimalistic bioink formulation is envisioned as a potential candidate for bone tissue engineering as a clinically translatable material with straightforward preparation and high cell activity.Article Citation - WoS: 23Citation - Scopus: 26Fish scale containing alginate dialdehyde-gelatin bioink for bone tissue engineering(IOP Publishing Ltd, 2023) Özenler, Aylin Kara; Distler, Thomas; Kara, Aylin; Tıhmınlıoğlu, Funda; 03.02. Department of Chemical Engineering; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyThe development of biomaterial inks suitable for biofabrication and mimicking the physicochemical properties of the extracellular matrix is essential for the application of bioprinting technology in tissue engineering (TE). The use of animal-derived proteinous materials, such as jellyfish collagen, or fish scale (FS) gelatin (GEL), has become an important pillar in biomaterial ink design to increase the bioactivity of hydrogels. However, besides the extraction of proteinous structures, the use of structurally intact FS as an additive could increase biocompatibility and bioactivity of hydrogels due to its organic (collagen) and inorganic (hydroxyapatite) contents, while simultaneously enhancing mechanical strength in three-dimensional (3D) printing applications. To test this hypothesis, we present here a composite biomaterial ink composed of FS and alginate dialdehyde (ADA)-GEL for 3D bioprinting applications. We fabricate 3D cell-laden hydrogels using mouse pre-osteoblast MC3T3-E1 cells. We evaluate the physicochemical and mechanical properties of FS incorporated ADA-GEL biomaterial inks as well as the bioactivity and cytocompatibility of cell-laden hydrogels. Due to the distinctive collagen orientation of the FS, the compressive strength of the hydrogels significantly increased with increasing FS particle content. Addition of FS also provided a tool to tune hydrogel stiffness. FS particles were homogeneously incorporated into the hydrogels. Particle-matrix integration was confirmed via scanning electron microscopy. FS incorporation in the ADA-GEL matrix increased the osteogenic differentiation of MC3T3-E1 cells in comparison to pristine ADA-GEL, as FS incorporation led to increased ALP activity and osteocalcin secretion of MC3T3-E1 cells. Due to the significantly increased stiffness and supported osteoinductivity of the hydrogels, FS structure as a natural collagen and hydroxyapatite source contributed to the biomaterial ink properties for bone engineering applications. Our findings indicate that ADA-GEL/FS represents a new biomaterial ink formulation with great potential for 3D bioprinting, and FS is confirmed as a promising additive for bone TE applications.Doctoral Thesis Development and Characterization of Novel Bioink by Using Decellularized Extracellular Matrix for Bone Tissue Engineering Applications(01. Izmir Institute of Technology, 2023) Kara, Aylin; Tıhmınlıoğlu, Funda; Tıhmınlıoğlu, Funda; Havıtçıoğlu, Hasan; 03.02. Department of Chemical Engineering; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyBone tissue engineering has focused on the development of functional scaffolds that can organize bone regeneration with appropriate structures and properties. Three-dimensional (3D) printing technology enables the development of personalized scaffolds. In addition, biological scaffolds obtained by decellularization have various advantages for developing natural-based scaffolds. The development of printable, patient-specific bioinks derived from decellularized extracellular matrix could provide 3D fabrication of tissues and organs with high potential to mimic native tissues. The presented thesis study demonstrates the development of various bioink compositions for bone tissue engineering applications. In this regard, bone tissues were decellularized with a novel method and then characterized in order to verify the removal of whole cellular components for eliminating immunological reactions. After the pulverization of tissues, decellularized bone (DB) particles were used as an additive within various ink combinations (alginate-, gelatin- and alginate-gelatin-based). Thus, various bioink formulations were developed containing DB particles, biopolymers and mesenchymal stem cells (MSC). All prepared bioinks were bioprinted, then the viability, proliferation and differentiation capacity of the cells inside the structures as well as the physical, rheological, and printability properties of the inks were assessed. The results revealed that all bioink combinations were suitable for bioprinting and the addition of DB particles improved cell proliferation and osteogenic differentiation in all bioink formulations. Alginate-based bioinks exhibited the greatest printability and shape fidelity, gelatin-based bioinks showed the highest cell proliferation and attachment, also, gelatin incorporation into alginate-based bioinks improved the biological activity of cells. In conclusion, cytocompatible, functional composite bioinks developed in this thesis study are of value for bone tissue engineering research in future to explore their functions in the living system and show complete bone regeneration while maintaining their stability for a long time.Article Citation - WoS: 51Citation - Scopus: 583d Printed Gelatin/Decellularized Bone Composite Scaffolds for Bone Tissue Engineering: Fabrication, Characterization and Cytocompatibility Study(Elsevier, 2022) Kara, Aylin; Distler, Thomas; Tıhmınlıoğlu, Funda; Kara, Aylin; Seitz, Hermann; Friedrich, Oliver; Tıhmınlıoğlu, Funda; Boccaccini, Aldo R; 03.02. Department of Chemical Engineering; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 01. Izmir Institute of TechnologyThree-dimensional (3D) printing technology enables the design of personalized scaffolds with tunable pore size and composition. Combining decellularization and 3D printing techniques provides the opportunity to fabricate scaffolds with high potential to mimic native tissue. The aim of this study is to produce novel decellularized bone extracellular matrix (dbECM)-reinforced composite-scaffold that can be used as a biomaterial for bone tissue engineering. Decellularized bone particles (dbPTs, ∼100 μm diameter) were obtained from rabbit femur and used as a reinforcement agent by mixing with gelatin (GEL) in different concentrations. 3D scaffolds were fabricated by using an extrusion-based bioprinter and crosslinking with microbial transglutaminase (mTG) enzyme, followed by freeze-drying to obtain porous structures. Fabricated 3D scaffolds were characterized morphologically, mechanically, and chemically. Furthermore, MC3T3-E1 mouse pre-osteoblast cells were seeded on the dbPTs reinforced GEL scaffolds (GEL/dbPTs) and cultured for 21 days to assess cytocompatibility and cell attachment. We demonstrate the 3D-printability of dbPTs-reinforced GEL hydrogels and the achievement of homogenous distribution of the dbPTs in the whole scaffold structure, as well as bioactivity and cytocompatibility of GEL/dbPTs scaffolds. It was shown that Young's modulus and degradation rate of scaffolds were enhanced with increasing dbPTs content. Multiphoton microscopy imaging displayed the interaction of cells with dbPTs, indicating attachment and proliferation of cells around the particles as well as into the GEL-particle hydrogels. Our results demonstrate that GEL/dbPTs hydrogel formulations have potential for bone tissue engineering.