Chromatographic Separation of Amyloglucosidase From the Mixtures of Enzymes
| dc.contributor.author | Harsa, Hayriye Şebnem | |
| dc.contributor.author | Furusaki, Shintaro | |
| dc.coverage.doi | 10.1016/S1369-703X(01)00123-1 | |
| dc.date.accessioned | 2016-05-03T08:46:19Z | |
| dc.date.available | 2016-05-03T08:46:19Z | |
| dc.date.issued | 2001 | |
| dc.description.abstract | An affinity chromatographic separation of amyloglucosidase (AMG) enzyme using β-cyclodextrin-chitosan matrix was investigated in this study. The adsorbent selectively adsorbed AMG from the mixtures of enzymes (lactase and α-amylase), and this selectively bound AMG was eluted from the column in two steps with a recovery yield of about 72%. A small fraction of AMG and other contaminating proteins did not bind to the column specifically and were eluted easily. Purified AMG was visualised on the sodiumdodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) experiments | en_US |
| dc.identifier.citation | Harsa, H. Ş., and Furusaki, S. (2001). Chromatographic separation of amyloglucosidase from the mixtures of enzymes. Biochemical Engineering Journal, 8(3), 257-261. doi:10.1016/S1369-703X(01)00123-1 | en_US |
| dc.identifier.doi | 10.1016/S1369-703X(01)00123-1 | en_US |
| dc.identifier.doi | 10.1016/S1369-703X(01)00123-1 | |
| dc.identifier.issn | 1369-703X | |
| dc.identifier.scopus | 2-s2.0-0034759362 | |
| dc.identifier.uri | https://doi.org/10.1016/S1369-703X(01)00123-1 | |
| dc.identifier.uri | https://hdl.handle.net/11147/4590 | |
| dc.language.iso | en | en_US |
| dc.publisher | Elsevier Ltd. | en_US |
| dc.relation.ispartof | Biochemical Engineering Journal | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.subject | Affinity | en_US |
| dc.subject | Bioseparations | en_US |
| dc.subject | Chromatography | en_US |
| dc.subject | Glucoamylase | en_US |
| dc.subject | Beta cyclodextrin | en_US |
| dc.title | Chromatographic Separation of Amyloglucosidase From the Mixtures of Enzymes | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication | |
| gdc.author.institutional | Harsa, Hayriye Şebnem | |
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| gdc.description.department | İzmir Institute of Technology. Food Engineering | en_US |
| gdc.description.endpage | 261 | en_US |
| gdc.description.issue | 3 | en_US |
| gdc.description.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| gdc.description.scopusquality | Q2 | |
| gdc.description.startpage | 257 | en_US |
| gdc.description.volume | 8 | en_US |
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| gdc.oaire.keywords | Chromatography | |
| gdc.oaire.keywords | Affinity | |
| gdc.oaire.keywords | Bioseparations | |
| gdc.oaire.keywords | Glucoamylase | |
| gdc.oaire.keywords | Beta cyclodextrin | |
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