Gene Reporter Assay To Validate Microrna Targets in Drosophila S2 Cells
| dc.contributor.author | Akgül, B. | |
| dc.contributor.author | Göktaş, C. | |
| dc.coverage.doi | 10.1007/978-1-62703-748-8_14 | |
| dc.date.accessioned | 2017-05-30T11:08:31Z | |
| dc.date.available | 2017-05-30T11:08:31Z | |
| dc.date.issued | 2014 | |
| dc.description.abstract | Bioinformatics programs have helped tremendously in identifying the targets of microRNAs, which are small noncoding RNAs that regulate gene expression posttranscriptionally. However, the partial complementarity between miRNAs and their targets hinders the accuracy of target prediction, necessitating the use of experimental validation procedures. Here, we describe a gene reporter assay typically used in our lab to validate putative miRNA-mRNA interactions in Drosophila S2 cells. © Springer Science+Business Media New York 2014. | en_US |
| dc.identifier.citation | Akgül, B., and Göktaş, Ç. (2014). Gene reporter assay to validate microRNA targets in drosophila S2 cells. Methods in Molecular Biology, 1107, 233-242. doi:10.1007/978-1-62703-748-8_14 | en_US |
| dc.identifier.doi | 10.1007/978-1-62703-748-8_14 | |
| dc.identifier.doi | 10.1007/978-1-62703-748-8_14 | en_US |
| dc.identifier.isbn | 9781627037471 | |
| dc.identifier.issn | 1064-3745 | |
| dc.identifier.scopus | 2-s2.0-84891796282 | |
| dc.identifier.uri | https://doi.org/10.1007/978-1-62703-748-8_14 | |
| dc.identifier.uri | https://hdl.handle.net/11147/5644 | |
| dc.language.iso | en | en_US |
| dc.publisher | Humana Press Inc. | en_US |
| dc.relation.ispartof | Methods in Molecular Biology | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.subject | Drosophila | en_US |
| dc.subject | Luciferase | en_US |
| dc.subject | Reporter Assay | en_US |
| dc.subject | S2 | en_US |
| dc.subject | Target Validation | en_US |
| dc.title | Gene Reporter Assay To Validate Microrna Targets in Drosophila S2 Cells | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication | |
| gdc.author.institutional | Akgül, Bünyamin | |
| gdc.author.institutional | Göktaş, Çağdaş | |
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| gdc.description.department | İzmir Institute of Technology | en_US |
| gdc.description.departmenttemp | Akgül B., Molecular Biology and Genetics, Izmir Institute of Technology, Izmir, Turkey; Göktaş C., Molecular Biology and Genetics, Izmir Institute of Technology, Izmir, Turkey | en_US |
| gdc.description.endpage | 242 | en_US |
| gdc.description.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
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| gdc.description.startpage | 233 | en_US |
| gdc.description.volume | 1107 | en_US |
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| gdc.oaire.keywords | MicroRNAs | |
| gdc.oaire.keywords | Base Sequence | |
| gdc.oaire.keywords | Molecular Sequence Data | |
| gdc.oaire.keywords | Reporter assay | |
| gdc.oaire.keywords | Animals | |
| gdc.oaire.keywords | Drosophila | |
| gdc.oaire.keywords | Luciferase | |
| gdc.oaire.keywords | Polymerase Chain Reaction | |
| gdc.oaire.keywords | Target validation | |
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