Fluorescent Protein With Environmentally-Sensitive Fluorescence Lifetime for Quantitative Ph Measurement
| dc.contributor.author | Simonyan, Tatiana R. | |
| dc.contributor.author | Protasova, Elena A. | |
| dc.contributor.author | Mamontova, Anastasia, V | |
| dc.contributor.author | Shakhov, Aleksander M. | |
| dc.contributor.author | Bodunova, Daria, V | |
| dc.contributor.author | Sidorenko, Svetlana, V | |
| dc.contributor.author | Bogdanov, Alexey M. | |
| dc.date.accessioned | 2025-03-25T22:56:03Z | |
| dc.date.available | 2025-03-25T22:56:03Z | |
| dc.date.issued | 2025 | |
| dc.description.abstract | Intracellular pH is a key factor in cell homeostasis, regulated within specific compartments, and changes in pH can result from or affect biochemical pathways. This study explores a yellow fluorescent protein EYFP-G65T as a core for a time-resolved pH-indicator. Among the tested designs-a circular permutant, a chimeric SypHer3s-like construct, and an unmodified protein-the unmodified EYFP-G65T performed best for live-cell imaging. Upon two-photon excitation, purified EYFP-G65T exhibited a 4.5-fold increase in mean fluorescence lifetime across pH 5.5-7 and a 7-fold change in its major component's lifetime from pH 6.5-8. Using this indicator, we measured pH values ranging from 6 to 8 in various organelles, and mapped pH shifts in mitochondria and the Golgi apparatus in response to stimuli. | en_US |
| dc.description.sponsorship | Russian Science Foundation [23-14-00042]; Semenov FRCCP RAS CCE [506694] | en_US |
| dc.description.sponsorship | FLIM experiments and part of the spectroscopic studies were sup-ported by the Russian Science Foundation (grant number 23-14-00042) . Experiments were partially carried out using equipment provided by the Semenov FRCCP RAS CCE (no.506694) . | en_US |
| dc.identifier.doi | 10.1016/j.abb.2025.110350 | |
| dc.identifier.issn | 0003-9861 | |
| dc.identifier.issn | 1096-0384 | |
| dc.identifier.scopus | 2-s2.0-85218173430 | |
| dc.identifier.uri | https://doi.org/10.1016/j.abb.2025.110350 | |
| dc.identifier.uri | https://hdl.handle.net/11147/15444 | |
| dc.language.iso | en | en_US |
| dc.publisher | Elsevier Science inc | en_US |
| dc.relation.ispartof | Archives of Biochemistry and Biophysics | |
| dc.rights | info:eu-repo/semantics/closedAccess | en_US |
| dc.subject | Fluorescence Lifetime | en_US |
| dc.subject | Flim | en_US |
| dc.subject | Ph Measurement | en_US |
| dc.subject | Genetically Encoded Indicators | en_US |
| dc.subject | Live-Cell Imaging | en_US |
| dc.title | Fluorescent Protein With Environmentally-Sensitive Fluorescence Lifetime for Quantitative Ph Measurement | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication | |
| gdc.author.wosid | Bogdanov, Alexey/S-2759-2018 | |
| gdc.author.wosid | Mamontova, Anastasia/L-5129-2016 | |
| gdc.author.wosid | Maksimov, Eugene/P-9185-2016 | |
| gdc.author.wosid | Simonyan, Tatiana/Itt-0632-2023 | |
| gdc.author.wosid | Shakhov, Aleksander/A-5439-2016 | |
| gdc.author.wosid | Protasova, Elena/Aaq-9536-2021 | |
| gdc.bip.impulseclass | C5 | |
| gdc.bip.influenceclass | C5 | |
| gdc.bip.popularityclass | C5 | |
| gdc.coar.access | metadata only access | |
| gdc.coar.type | text::journal::journal article | |
| gdc.collaboration.industrial | false | |
| gdc.description.department | İzmir Institute of Technology | en_US |
| gdc.description.departmenttemp | [Simonyan, Tatiana R.; Bogdanov, Alexey M.] Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia; [Protasova, Elena A.; Sidorenko, Svetlana, V; Maksimov, Eugene G.] MV Lomonosov Moscow State Univ, Fac Biol, Moscow 119992, Russia; [Shakhov, Aleksander M.] NN Semenov Fed Res Ctr Chem Phys, Bio &Nanophoton Lab, Moscow 119991, Moscow, Russia; [Bogdanov, Alexey M.] Izmir Inst Technol, Dept Photon, TR-35430 Izmir, Turkiye | en_US |
| gdc.description.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| gdc.description.scopusquality | Q2 | |
| gdc.description.volume | 766 | en_US |
| gdc.description.woscitationindex | Science Citation Index Expanded | |
| gdc.description.wosquality | Q2 | |
| gdc.identifier.openalex | W4407675881 | |
| gdc.identifier.pmid | 39971109 | |
| gdc.identifier.wos | WOS:001431939700001 | |
| gdc.index.type | WoS | |
| gdc.index.type | Scopus | |
| gdc.index.type | PubMed | |
| gdc.oaire.diamondjournal | false | |
| gdc.oaire.impulse | 0.0 | |
| gdc.oaire.influence | 2.635068E-9 | |
| gdc.oaire.isgreen | false | |
| gdc.oaire.keywords | Luminescent Proteins | |
| gdc.oaire.keywords | Bacterial Proteins | |
| gdc.oaire.keywords | Humans | |
| gdc.oaire.keywords | Golgi Apparatus | |
| gdc.oaire.keywords | Hydrogen-Ion Concentration | |
| gdc.oaire.keywords | Fluorescence | |
| gdc.oaire.keywords | HeLa Cells | |
| gdc.oaire.keywords | Mitochondria | |
| gdc.oaire.popularity | 2.1091297E-10 | |
| gdc.oaire.publicfunded | false | |
| gdc.openalex.collaboration | International | |
| gdc.openalex.fwci | 0.0 | |
| gdc.openalex.normalizedpercentile | 0.03 | |
| gdc.opencitations.count | 0 | |
| gdc.plumx.mendeley | 1 | |
| gdc.plumx.scopuscites | 0 | |
| gdc.scopus.citedcount | 0 | |
| gdc.wos.citedcount | 0 | |
| relation.isAuthorOfPublication.latestForDiscovery | 9acd77d0-09dd-4acf-a74a-be6f341754c7 | |
| relation.isOrgUnitOfPublication.latestForDiscovery | 9af2b05f-28ac-4010-8abe-a4dfe192da5e |
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