Protocol for Cell Surface Biotinylation of Magnetic Labeled and Captured Human Peripheral Blood Mononuclear Cells
| dc.contributor.author | Ayaz Güner, Şerife | |
| dc.contributor.author | Acar, Mustafa Burak | |
| dc.contributor.author | Boyvat, Dudu | |
| dc.contributor.author | Güner, Hüseyin | |
| dc.contributor.author | Bozalan, Habibe | |
| dc.contributor.author | Güzel, Melis | |
| dc.contributor.author | Yıldır, Selin Kübra | |
| dc.contributor.author | Altınsoy, Nilay | |
| dc.contributor.author | Fındık, Fatma | |
| dc.contributor.author | Karakükçü, Musa | |
| dc.contributor.author | Özcan, Servet | |
| dc.date.accessioned | 2022-12-09T12:45:22Z | |
| dc.date.available | 2022-12-09T12:45:22Z | |
| dc.date.issued | 2022 | |
| dc.description.abstract | Analysis of the surfaceome of a blood cell subset requires cell sorting, followed by surface protein enrichment. Here, we present a protocol combining magnetically activated cell sorting (MACS) and surface biotinylation of the target cell subset from human peripheral blood mononuclear cells (PBMCs). We describe the steps for isolating target cells and their in-column surface biotinylation, followed by isolation and mass spectrometry analysis of biotinylated proteins. The protocol enables in-column surface biotinylation of specific cell subsets with minimal membrane disruption. | en_US |
| dc.identifier.doi | 10.1016/j.xpro.2022.101863 | |
| dc.identifier.issn | 2666-1667 | en_US |
| dc.identifier.scopus | 2-s2.0-85142326332 | |
| dc.identifier.uri | https://doi.org/10.1016/j.xpro.2022.101863 | |
| dc.identifier.uri | https://hdl.handle.net/11147/12662 | |
| dc.language.iso | en | en_US |
| dc.publisher | Elsevier | en_US |
| dc.relation.ispartof | STAR Protocols | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.subject | Cell biology | en_US |
| dc.subject | Cell isolation | en_US |
| dc.subject | Mass spectrometry | en_US |
| dc.subject | Protein biochemistry | en_US |
| dc.subject | Proteomics | en_US |
| dc.title | Protocol for Cell Surface Biotinylation of Magnetic Labeled and Captured Human Peripheral Blood Mononuclear Cells | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication | |
| gdc.author.id | 0000-0002-1052-0961 | |
| gdc.author.institutional | Ayaz Güner, Şerife | |
| gdc.bip.impulseclass | C5 | |
| gdc.bip.influenceclass | C5 | |
| gdc.bip.popularityclass | C5 | |
| gdc.coar.access | open access | |
| gdc.coar.type | text::journal::journal article | |
| gdc.collaboration.industrial | false | |
| gdc.description.department | İzmir Institute of Technology. Molecular Biology and Genetics | en_US |
| gdc.description.issue | 4 | en_US |
| gdc.description.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| gdc.description.scopusquality | Q4 | |
| gdc.description.volume | 3 | en_US |
| gdc.description.wosquality | Q4 | |
| gdc.identifier.openalex | W4309503254 | |
| gdc.identifier.pmid | 36595912 | |
| gdc.identifier.wos | WOS:001059416400008 | |
| gdc.index.type | WoS | |
| gdc.index.type | Scopus | |
| gdc.oaire.accesstype | GOLD | |
| gdc.oaire.diamondjournal | false | |
| gdc.oaire.downloads | 44 | |
| gdc.oaire.impulse | 3.0 | |
| gdc.oaire.influence | 2.7414127E-9 | |
| gdc.oaire.isgreen | true | |
| gdc.oaire.keywords | Proteomics | |
| gdc.oaire.keywords | Science (General) | |
| gdc.oaire.keywords | Magnetic Phenomena | |
| gdc.oaire.keywords | Cell Membrane | |
| gdc.oaire.keywords | Membrane Proteins | |
| gdc.oaire.keywords | Cell Biology | |
| gdc.oaire.keywords | Mass Spectrometry | |
| gdc.oaire.keywords | Q1-390 | |
| gdc.oaire.keywords | Protein Biochemistry | |
| gdc.oaire.keywords | Protocol | |
| gdc.oaire.keywords | Leukocytes, Mononuclear | |
| gdc.oaire.keywords | Cell Isolation | |
| gdc.oaire.keywords | Humans | |
| gdc.oaire.keywords | Biotinylation | |
| gdc.oaire.popularity | 2.2403313E-9 | |
| gdc.oaire.publicfunded | false | |
| gdc.oaire.sciencefields | 0301 basic medicine | |
| gdc.oaire.sciencefields | 0303 health sciences | |
| gdc.oaire.sciencefields | 03 medical and health sciences | |
| gdc.oaire.views | 133 | |
| gdc.openalex.collaboration | International | |
| gdc.openalex.fwci | 0.5841186 | |
| gdc.openalex.normalizedpercentile | 0.57 | |
| gdc.opencitations.count | 2 | |
| gdc.plumx.crossrefcites | 3 | |
| gdc.plumx.mendeley | 17 | |
| gdc.plumx.pubmedcites | 1 | |
| gdc.plumx.scopuscites | 4 | |
| gdc.scopus.citedcount | 4 | |
| gdc.wos.citedcount | 4 | |
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