Identification of Staphylococci by 16s Internal Transcribed Spacer Rrna Gene Restriction Fragment Length Polymorphism
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Abstract
The capacity of 16S internal transcribed spacer (16S-ITS) rRNA gene RFLP to differentiate 16 type strains and nine clinical isolates of staphylococci was evaluated. The 16S rRNA gene was amplified together with the ITS region and the amplification products were digested with TaqI restriction enzyme. Analysis of the 16S-ITS rRNA gene RFLP profiles differentiated each of the 16 type strains into distinct RFLP haplotypes.
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Keywords
DNA determination, Bacterial typing techniques, Bacterium isolation, Polymerase chain reaction, Ribosomal spacer, RNA analysis, DNA, Bacterial, Bacterial typing techniques, Staphylococcus, DNA determination, Genes, rRNA, RNA analysis, Polymerase Chain Reaction, Polymerase chain reaction, Bacterial Typing Techniques, Species Specificity, RNA, Ribosomal, 16S, DNA, Ribosomal Spacer, Ribosomal spacer, Animals, Humans, Bacterium isolation, Polymorphism, Restriction Fragment Length
Fields of Science
0301 basic medicine, 0303 health sciences, 03 medical and health sciences, 0103 physical sciences, 01 natural sciences
Citation
Sudağıdan, M., Yenidunya, A.F., and Güneş, H. (2005). Identification of staphylococci by 16S internal transcribed spacer rRNA gene restriction fragment length polymorphism. Journal of Medical Microbiology, 54(9), 823-826. doi:10.1099/jmm.0.45868-0
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13
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54
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9
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823
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826
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