Partieal Purification and Characterization of Lipase Enzyme From a Pseudomonas Strain

Abstract

Lipase is a triacylglycerol-hydrolyzing enzyme which is catalyzed the hydrolysis of water insoluble free fatty acid and glycerols and also a wide range of chemical reactions. Beside, microbial lipases show regiospecificity and enantioselectivity properties. Therefore, microbial lipases gain the great importance for industrial applications and organic synthesis. In this study, investigation, partial purification and characterization of lipase enzyme from a Pseudomonas strain was studied by using different analytical approach.Purification step was done by size-exclusion chromatography. The molecularweight of partial purified lipase was determined by SDS-PAGE. Spectrophotometric lipase assay applied to find out the enzyme characterization. Kinetic study of enzyme was also investigated varying the substrates concentrations. Specific activity staining on gel procedures applied after native gel process. After electrophoresis, lipase activity responsive protein bands were appeared on gel.After screening for the presence of lipase activity in Pseudonomas strain which was isolated from soil, it was decided to choose intracellular enzyme sample for characterization and purification studies. The enzyme gave the highest lipase activity when p-nitrophenyl laurate used as a substrate. The optimum pH range for activity of lipase was alkaline pH ranges, about pH 8.0 and 9.0. The optimum temperature was dedicated as 25oC. In the presence of metal salts and organic solvents; while some additives sharply decreased enzyme activity, some additives were not effect the enzyme activity. Approximate molecular mass of partially purified enzyme was between 29 kDa and 43 kDa.