Salt Stress Responsive Proteins Identification in Wild Sugar Beet (beta Maritima) by Mass Spectrometry

Abstract

Salt stress is one of the major abiotic stresses in agriculture worldwide. Seven percent of the land.s surface and five percent of cultivated lands are affected by salinity.Turkey is the fourth in the world and third in Europe in producing sugar beet. It is observed that salt stress affects the sugar beet negatively especially at germination and seedling stages, it limits the productivity of crop plants and affects the quality of plants.In the present study, proteomic approach was used to investigate the salt-stress responsive proteins in wild salt-tolerant beet, Beta maritima. Sugar beet were grown approximately two months. After growing, they were treated with 250 mM NaCl for seven days. Control plants received no salt treatment during this period. Total proteins of leaves and root were extracted. The proteins were fragmented into peptides using insolution digestion technique and liquid chromatography-tandem mass spectrometry (LC-MS/MS) used for identified the proteins. Totally 288 proteins were identified in leave samples and totally 259 proteins were identified in the root samples.Identified protein results were shown that unique of salt leave proteins and upregulated proteins of leave samples were the related to the antioxidant enzymes. On the other hand, active transporter protein of vacular ATP synthase subunit A was identified in the salt responsive of root samples.