Conjugation of Sirna With Comb-Type Peg Enhances Serum Stability and Gene Silencing Efficiency
| dc.contributor.author | Gunasekaran, Karthikeyan | |
| dc.contributor.author | Nguyen, Thi H. | |
| dc.contributor.author | Maynard, Heather D. | |
| dc.contributor.author | Davis, Thomas P. | |
| dc.contributor.author | Bulmuş, Volga | |
| dc.coverage.doi | 10.1002/marc.201000804 | |
| dc.date.accessioned | 2017-03-17T11:13:23Z | |
| dc.date.available | 2017-03-17T11:13:23Z | |
| dc.date.issued | 2011 | |
| dc.description.abstract | A thiol-modified siRNA targeting the enhanced green fluorescence protein (eGFP) gene was conjugated with RAFT-synthesized, pyridyl disulfide-functional poly(PEG methyl ether acrylate)s (p(PEGA)s). siRNA-p(PEGA) conjugates demonstrated significantly enhanced in vitro serum stability and nuclease resistance compared to the unmodified and thiol-modified siRNA. The complexes of siRNA-p(PEGA) conjugates with a fusogenic peptide, KALA ((+)/(-) = 2) inhibited the protein expression approximately 28-fold more than the KALA complex of the unmodified siRNA. The protein inhibition caused by siRNA-p(PEGA)-KALA complexes (56± 5%-58± 3% of the fluorescence expressed in non-treated cells) was comparable to the effect of the unmodified siRNA-lipofectamine complex (77± 7%). | en_US |
| dc.identifier.citation | Gunasekaran, K., Nguyen, T.H., Maynard, H.D., Davis, T.P., and Bulmuş, V. (2001). Conjugation of siRNA with comb-type PEG enhances serum stability and gene silencing efficiency. Macromolecular Rapid Communications, 32(8), 654-659. doi:10.1002/marc.201000804 | en_US |
| dc.identifier.doi | 10.1002/marc.201000804 | en_US |
| dc.identifier.doi | 10.1002/marc.201000804 | |
| dc.identifier.issn | 1022-1336 | |
| dc.identifier.issn | 1521-3927 | |
| dc.identifier.scopus | 2-s2.0-79953844934 | |
| dc.identifier.uri | https://doi.org/10.1002/marc.201000804 | |
| dc.identifier.uri | https://hdl.handle.net/11147/5083 | |
| dc.language.iso | en | en_US |
| dc.publisher | John Wiley and Sons Inc. | en_US |
| dc.relation.ispartof | Macromolecular Rapid Communications | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.subject | PEG(meth)acrylate | en_US |
| dc.subject | siRNA | en_US |
| dc.subject | Biological applications of polymers | en_US |
| dc.subject | Polymer conjugates | en_US |
| dc.subject | RAFT polymerization | en_US |
| dc.subject | Reversible addition fragmentation chain transfer polymerization | en_US |
| dc.title | Conjugation of Sirna With Comb-Type Peg Enhances Serum Stability and Gene Silencing Efficiency | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication | |
| gdc.author.institutional | Bulmuş, Volga | |
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| gdc.description.department | İzmir Institute of Technology. Chemical Engineering | en_US |
| gdc.description.endpage | 659 | en_US |
| gdc.description.issue | 8 | en_US |
| gdc.description.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
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| gdc.description.startpage | 654 | en_US |
| gdc.description.volume | 32 | en_US |
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| gdc.identifier.pmid | 21480423 | |
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| gdc.oaire.keywords | Serum | |
| gdc.oaire.keywords | Design | |
| gdc.oaire.keywords | Polymers | |
| gdc.oaire.keywords | Living Radical Polymerization | |
| gdc.oaire.keywords | RNA Stability | |
| gdc.oaire.keywords | Green Fluorescent Proteins | |
| gdc.oaire.keywords | Cell Line | |
| gdc.oaire.keywords | Polyethylene Glycols | |
| gdc.oaire.keywords | Ribonucleases | |
| gdc.oaire.keywords | Cell Line, Tumor | |
| gdc.oaire.keywords | Humans | |
| gdc.oaire.keywords | Biological applications of polymers | |
| gdc.oaire.keywords | Gene Silencing | |
| gdc.oaire.keywords | RNA, Small Interfering | |
| gdc.oaire.keywords | Vegf Sirna | |
| gdc.oaire.keywords | RAFT polymerization | |
| gdc.oaire.keywords | Polymer conjugates | |
| gdc.oaire.keywords | Poly(Ethylene Glycol)-Sirna Conjugate | |
| gdc.oaire.keywords | Polyelectrolyte Complex Micelles | |
| gdc.oaire.keywords | Genetic Therapy | |
| gdc.oaire.keywords | Pegylation | |
| gdc.oaire.keywords | PEG(meth)acrylate | |
| gdc.oaire.keywords | Reversible Addition | |
| gdc.oaire.keywords | siRNA | |
| gdc.oaire.keywords | Peptide | |
| gdc.oaire.keywords | Delivery | |
| gdc.oaire.keywords | Reversible addition fragmentation chain transfer polymerization | |
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