Protein Quantification Via Lspr-Based Biosensor Platform Utilizing Chrono-Growth for Enhanced Sensitivity
| dc.contributor.author | Sozmen, A. B. | |
| dc.contributor.author | Arslan-Yildiz, A. | |
| dc.date.accessioned | 2024-07-02T13:33:04Z | |
| dc.date.available | 2024-07-02T13:33:04Z | |
| dc.date.issued | 2024 | |
| dc.description.abstract | In this study an enhancement methodology, which utilizes time dependent growth of immobilized gold nanoparticles (GNPs) for LSPR-based biosensor platform was developed. The chrono-growth methodology was used for protein analysis and quantification. The method consisted GNP immobilization onto well-plates, GNP chronogrowth, and antibody functionalization. Success of each step was verified by UV-Vis spectrum measurement. Afterwards, the biosensor platform was tested to determine its characteristics. Bovine Serum Albumin (BSA) was chosen to be used as a model protein and an LoD value of 0.344 mu M and a dynamic detection range of 1 to 1000 mu M was calculated. The results were acquired within 30 min. Developed platform provides simple and rapid detection of the protein. | en_US |
| dc.description.sponsorship | Türkiye Bilimsel ve Teknolojik Araştırma Kurumu, TÜBİTAK, (221M589) | en_US |
| dc.description.sponsorship | Scientific and Technological Research Council of Turkiye (TUBITAK) [221M589] | en_US |
| dc.description.sponsorship | This study was funded by The Scientific and Technological Research Council of Turkiye (TUBITAK; 221M589) . | en_US |
| dc.identifier.doi | 10.1016/j.matlet.2024.136782 | |
| dc.identifier.issn | 0167-577X | |
| dc.identifier.issn | 1873-4979 | |
| dc.identifier.scopus | 2-s2.0-85196422333 | |
| dc.identifier.uri | https://doi.org/10.1016/j.matlet.2024.136782 | |
| dc.identifier.uri | https://hdl.handle.net/11147/14622 | |
| dc.language.iso | en | en_US |
| dc.publisher | Elsevier | en_US |
| dc.relation.ispartof | Materials Letters | en_US |
| dc.rights | info:eu-repo/semantics/closedAccess | en_US |
| dc.subject | Localized surface plasmon resonance | en_US |
| dc.subject | Biosensors | en_US |
| dc.subject | Gold Nanoparticles | en_US |
| dc.subject | Protein Detection | en_US |
| dc.title | Protein Quantification Via Lspr-Based Biosensor Platform Utilizing Chrono-Growth for Enhanced Sensitivity | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication | |
| gdc.author.scopusid | 57201620092 | |
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| gdc.coar.access | metadata only access | |
| gdc.coar.type | text::journal::journal article | |
| gdc.collaboration.industrial | false | |
| gdc.description.department | Izmir Institute of Technology | en_US |
| gdc.description.departmenttemp | [Sozmen, A. B.; Arslan-Yildiz, A.] Izmir Inst Technol, Bioengn Dept Izmir, Urla, Turkiye | en_US |
| gdc.description.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| gdc.description.scopusquality | Q2 | |
| gdc.description.volume | 370 | en_US |
| gdc.description.woscitationindex | Science Citation Index Expanded | |
| gdc.description.wosquality | Q3 | |
| gdc.identifier.openalex | W4399560730 | |
| gdc.identifier.wos | WOS:001348567900001 | |
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