Optimization of Pulsed Field Gel Electrophoresis (pfge) Conditions for Thermophilic Bacilli
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BRONZE
Green Open Access
Yes
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No
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Abstract
Although thermophilic members of the genus Bacillus are known to tolerate extreme environmental conditions, they appeared to be readily damaged upon mechanical manipulations. This fact was evidenced in genotyping of some strains of thermophilic Bacillus by pulsed field gel electrophoresis (PFGE). Consequently, a new procedure for the preparation of agarose plugs was developed. The procedure produced interpretable genomic DNA restriction profiles.
Description
Keywords
DNA degradation, Genotyping, Pulsed field gel electrophoresis, Bacteriology, Thermophilic bacilli, Genotyping, Pulsed field gel electrophoresis, DNA degradation, Thermophilic bacilli, Bacteriology
Fields of Science
0301 basic medicine, 0303 health sciences, 03 medical and health sciences
Citation
Yavuz, E., Güneş, H., Harsa, H. Ş., Bulut, Ç., and Yenidünya, A. F. (2004). Optimization of pulsed field gel electrophoresis (PFGE) conditions for thermophilic bacilli. World Journal of Microbiology and Biotechnology, 20(8), 871-874. doi:10.1007/s11274-004-1004-3
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Scopus Q
OpenCitations Citation Count
2
Volume
20
Issue
8
Start Page
871
End Page
874
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CrossRef : 1
Scopus : 3
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