Magnetic Levitational Assembly of Differentiated Sh-Sy5y Cells for Aβ-Induced 3d Alzheimer's Disease Modeling and Curcumin Screening
| dc.contributor.author | Bilginer-Kartal, Rumeysa | |
| dc.contributor.author | Arslan-Yildiz, Ahu | |
| dc.date.accessioned | 2025-04-25T20:33:46Z | |
| dc.date.available | 2025-04-25T20:33:46Z | |
| dc.date.issued | 2025 | |
| dc.description.abstract | Alzheimer's disease is one of the prevalent neurodegenerative diseases and is characterized by amyloid beta aggregate (A beta) accumulation. This study reports an A beta 1-42 induced 3D Alzheimer's disease modeling utilizing differentiated SH-SY5Y spheroids, which is carried out by Magnetic levitation approach, and the neuroprotective effect of Curcumin is further investigated on this model. For this purpose, SH-SY5Y spheroids are differentiated using Retinoic acid-Brain-derived neurotrophic factor sequentially during 3D cell culture. Differentiated spheroids maintained high viability and exhibited significant neuronal characteristics, as evidenced by increasing beta-III tubulin and NeuN expressions. 3D Alzheimer's disease model formation and neurotoxicity of A beta 1-42 aggregates are investigated on un-/differentiated spheroids, resulting in 65% and 51% cell viability, respectively. Characterization of the 3D Alzheimer's disease model is done by immunostaining of Choline acetyltransferase to investigate cholinergic neuron activity loss, showing a 2.2 decrease in fluorescence intensity. Further, Curcumin treatment on the 3D Alzheimer's disease model resulted in augmenting cell viability, confirming neuroprotective effect of Curcumin on A beta 1-42 induced Alzheimer's disease model. This study highlighted the magnetic levitation-based fabrication of A beta 1-42-induced 3D Alzheimer's disease model successfully, offering a promising experimental platform for other neurodegenerative disease research and potential clinical applications. | en_US |
| dc.description.sponsorship | Scientific and Technological Research Council of Turkey (TUBITAK) [2211-A]; Turkish Academy of Sciences (TUEBA); IZTECH-Scientific Research Project (IYTE BAP) [2022IYTE-1-0058] | en_US |
| dc.description.sponsorship | The authors acknowledge & Idot;zmir Institute of Technology Biotechnology and Bioengineering Research and Application Center. The authors would like to thank Prof. Dr. P & imath;nar Akan for the fruitful discussion on the development of the Alzheimer's disease model. Rumeysa Bilginer-Kartal gratefully acknowledges the Scientific and Technological Research Council of Turkey (TUBITAK) 2211-A National Graduate Scholarship program. This study has been supported by the Turkish Academy of Sciences (TUEBA), in the framework of the Young Scientist Award Program (TUEBA-GEB & Idot;P/2019) and IZTECH-Scientific Research Project (IYTE BAP Grant no: 2022IYTE-1-0058). | en_US |
| dc.identifier.doi | 10.1002/mabi.202400658 | |
| dc.identifier.issn | 1616-5187 | |
| dc.identifier.issn | 1616-5195 | |
| dc.identifier.scopus | 2-s2.0-105000998689 | |
| dc.identifier.uri | https://doi.org/10.1002/mabi.202400658 | |
| dc.identifier.uri | https://hdl.handle.net/11147/15527 | |
| dc.language.iso | en | en_US |
| dc.publisher | Wiley-v C H verlag Gmbh | en_US |
| dc.relation.ispartof | Macromolecular Bioscience | |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.subject | 3D Alzheimer'S Disease Model | en_US |
| dc.subject | A Beta 1-42 Aggregates | en_US |
| dc.subject | Curcumin Screening | en_US |
| dc.subject | Differentiated Sh-Sy5Y Spheroids | en_US |
| dc.subject | Magnetic Levitation | en_US |
| dc.title | Magnetic Levitational Assembly of Differentiated Sh-Sy5y Cells for Aβ-Induced 3d Alzheimer's Disease Modeling and Curcumin Screening | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication | |
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| gdc.description.department | İzmir Institute of Technology | en_US |
| gdc.description.departmenttemp | [Bilginer-Kartal, Rumeysa; Arslan-Yildiz, Ahu] Izmir Inst Technol IZTECH, Dept Bioengn, TR-35430 Izmir, Turkiye | en_US |
| gdc.description.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
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| gdc.description.volume | 25 | |
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