Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7148
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Article Citation - WoS: 3Citation - Scopus: 3Optimization of Pulsed Field Gel Electrophoresis (pfge) Conditions for Thermophilic Bacilli(Springer Verlag, 2004) Yavuz, Elif; Güneş, Hatice; Yavuz, Elif; Bulut, Çisem; Yenidünya, Ali Fazıl; Yenidünya, Ali Fazıl; Harsa, Hayriye Şebnem; Güneş, Hatice; 04.03. Department of Molecular Biology and Genetics; 01. Izmir Institute of Technology; 03.08. Department of Food Engineering; 03. Faculty of Engineering; 04. Faculty of ScienceAlthough thermophilic members of the genus Bacillus are known to tolerate extreme environmental conditions, they appeared to be readily damaged upon mechanical manipulations. This fact was evidenced in genotyping of some strains of thermophilic Bacillus by pulsed field gel electrophoresis (PFGE). Consequently, a new procedure for the preparation of agarose plugs was developed. The procedure produced interpretable genomic DNA restriction profiles.
