Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
Permanent URI for this collectionhttps://hdl.handle.net/11147/7148
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Article Citation - WoS: 4Citation - Scopus: 4Novel Electrospun-Based Extractive Probes for Rapid Determination of Clinically Important Compounds in Human Plasma(Elsevier B.V., 2024) Temel,E.R.; Eroğlu,A.E.; Salih,B.; Boyaci,E.Background: Coated blade spray (CBS) represents an innovative approach that utilizes solid-phase microextraction principles for sampling and sample preparation. When combined with ambient mass spectrometry (MS), it can also serve as an electrospray ionization source. Therefore, it became a promising tool in analytical applications as it can significantly reduce the analysis time. However, the current CBS coatings are based on the immobilization of extractive particles in bulk polymeric glue, which constrains the diffusion of the analytes to reach the extractive phase; therefore, the full reward of the system cannot be taken at pre-equilibrium. This has sparked the notion of developing new CBS probes that exhibit enhanced kinetics. Results: With this aim, to generate a new extractive phase with improved extraction kinetics, poly(divinylbenzene) (PDVB) nanoparticles were synthesized by mini-emulsion polymerization and then immobilized into sub-micrometer (in diameter) sized polyacrylonitrile fibers which were obtained by electrospinning method. Following the optimization and characterization studies, the electrospun-coated blades were used to determine cholesterol, testosterone, and progesterone in plasma spots using the CBS-MS approach. For testosterone and progesterone, 10 ng mL−1 limits of quantification could be obtained, which was 200 ng mL−1 for cholesterol in spot-sized samples without including any pre-treatment steps to samples prior to extraction. Significance: The comparison of the initial kinetics for dip-coated and electrospun-coated CBS probes proved that the electrospinning process could enhance the extraction kinetics; therefore, it can be used for more sensitive analyses. The total analysis time with this method, from sample preparation to instrumental analysis, takes only 7 min, which suggests that the new probes are promising for fast diagnostic applications. © 2024 Elsevier B.V.Book Part Citation - Scopus: 1Mass Spectroscopy in Polymer Research(John Wiley and Sons Inc., 2012) Hacaloğlu, Jale; Yalçın, TalatMass spectrometers are currently available in numerous confi gurations with various ionization techniques and mass analyzers. For a specifi c polymer sample, an optimal choice should be done depending on the information sought. In practice, no single mass spectrometric technique can provide all necessary information for structural, compositional, and thermal and oxidative degradation characteristics of polymeric samples. The analyzer used determines the limits of the sensitivity and resolution and the mass range, whereas the type of ionization source specifi es the information that can be gained.Conference Object Citation - Scopus: 1Ranking Tandem Mass Spectra: and the Impact of Database Size and Scoring Function on Peptide Spectrum Matches(Institute of Electrical and Electronics Engineers Inc., 2013) Has, Canan; Kundakçı, Cemal Ulaş; Altay, Aybuge; Allmer, JensProteomics is currently driven by mass spectrometry. For the analysis of tandem mass spectra many computational algorithms have been proposed. There are two approaches, one which assigns a peptide sequence to a tandem mass spectrum directly and one which employs a sequence database for looking up possible solutions. The former method needs high quality spectra while the latter can tolerate lower quality spectra. Since both methods are computationally expensive, it is sensible to establish spectral quality using an independent fast algorithm. In this study, we first establish proper settings for database search algorithms for the analysis of spectra in our gold benchmark dataset and then analyze the performance of ScanRanker, an algorithm for quality assessment of tandem MS spectra, on this ground truth data. We found that OMSSA and MSGFDB have limitations in their scoring functions but were able to form a proper consensus prediction using majority vote for our benchmark data. Unfortunately, ScanRanker's results do not correlate well with the consensus and ScanRanker is also too slow to be used in the capacity it is supposed to be used. © 2013 IEEEConference Object Relative Protein Quantitation With Post Translational Modifications in Mass Spectrometry Based Proteomics(Institute of Electrical and Electronics Engineers Inc., 2010) Allmer, JensMass spectrometry has become the tool of choice for most investigations in proteomics. Identification of proteins from complex mixtures has long been achieved and is now routinely used in countless high throughput studies. Quantitation by mass spectrometry is comparably newer and many different strategies have been proposed. One such strategy quantitates the difference in protein expression level among samples via extracted ion chromatograms, or spectral counts or a combination thereof. Another strategy involves mass modifications of the analytes in one or more of the samples under investigation. MSMAG has been developed as an extension to 2DB and it has been shown that it can aid in quantitation of data from experiments employing label-free quantitation. Recently, it has been extended to allow for analysis of data based on labelling strategies. This also makes it possible to quickly visualize and investigate inherent mass differences as presented by post translational modifications. ©2009 IEEE.