Master Degree / Yüksek Lisans Tezleri
Permanent URI for this collectionhttps://hdl.handle.net/11147/3008
Browse
Search Results
Master Thesis Investigation of Heat Stress-Induced Proteins of Cold-Adapted Pseudomonas Marginals Using Proteomic Approach(Izmir Institute of Technology, 2008) Taşoğlu, Çağdaş; Yalçın, TalatTemperature alteration is known as a common environmental stress condition which all living organisms encounter and response by producing evolutionary wellconserved specific proteins called heat stress or heat shock proteins in the cell in order to adapt and survive. In the current study, the induction of heat stress proteins in a coldadapted bacterial strain of Pseudomonas marginalis cells grown under heat stress was investigated by proteomic approach. Five different temperatures, 5, 10, 15, 24, and 30C, were examined for the purpose of determining the optimum growth temperature for the bacterium. Consequently, 15°C was observed as optimum temperature for growth while 30C was established as heat stress temperature. Total proteins from Pseudomonas marginalis cells in the late exponential phase of growth at these two temperatures were extracted and separated by two-dimensional polyacrylamide gel electrophoresis. Totally 1391 protein spots were visualized for 15C and 1384 protein spots for 30C. After comparing with 15C, 13 protein spots that were differentially expressed in the cells exposed to heat stress (30C) were cut from the gel and fragmented into their peptides by in-gel digestion method. Finally, these proteins were identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and database searching. Among them, ribosome recycling factor, universal stress protein family and chaperonin GroEL were established as direct sensors of heat stress. As a result, the genes encoding these two heat stress proteins can be isolated and cloned into any other useful microorganism such as bacteria used for detoxification of industrial waste or used in bioremediation but not capable of surviving at high temperatures so that they can be efficient at those temperatures, too.Master Thesis Salt Stress Responsive Proteins Identification in Wild Sugar Beet (beta Maritima) by Mass Spectrometry(Izmir Institute of Technology, 2008) Baydara, Emine Pınar; Yalçın, TalatSalt stress is one of the major abiotic stresses in agriculture worldwide. Seven percent of the land.s surface and five percent of cultivated lands are affected by salinity.Turkey is the fourth in the world and third in Europe in producing sugar beet. It is observed that salt stress affects the sugar beet negatively especially at germination and seedling stages, it limits the productivity of crop plants and affects the quality of plants.In the present study, proteomic approach was used to investigate the salt-stress responsive proteins in wild salt-tolerant beet, Beta maritima. Sugar beet were grown approximately two months. After growing, they were treated with 250 mM NaCl for seven days. Control plants received no salt treatment during this period. Total proteins of leaves and root were extracted. The proteins were fragmented into peptides using insolution digestion technique and liquid chromatography-tandem mass spectrometry (LC-MS/MS) used for identified the proteins. Totally 288 proteins were identified in leave samples and totally 259 proteins were identified in the root samples.Identified protein results were shown that unique of salt leave proteins and upregulated proteins of leave samples were the related to the antioxidant enzymes. On the other hand, active transporter protein of vacular ATP synthase subunit A was identified in the salt responsive of root samples.