Electrical - Electronic Engineering / Elektrik - Elektronik Mühendisliği
Permanent URI for this collectionhttps://hdl.handle.net/11147/11
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Article Synthesis and Characterization of Pollen Extract Mediated Gold Nanostructures(Bingöl Üniversitesi Fen Bilimleri Enstitüsü, 2020) Bakar, Fatma; Sönmez, Hamide; Evecen, Senanur; Turan, Buse; Demir, Mehmet; Gümüş, Abdurrahman; Çeter, Talip; Yazgan, İdrisThere is an increasing demand in the synthesis of shape and size-controlled gold nanostructures (Au NSs) with greener methods. Therefore, we aimed to synthesize differently shaped and sized Au NSs using a greener technique under ambient conditions. In this study, we utilized pollen extracts of Corylus avellana, Juniperus oxycedrus and Pinus nigra species (collected from Kastamonu region of Turkey) for the synthesis. The extraction was performed in water in order to recover water soluble content from the pollen grains. The extracts were used to stabilize, and shape/size direct the HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) buffer synthesized Au NSs. UV-vis, powder X-ray diffraction (PXRD), scanning electron microscopy (SEM) characterizations proved synthesis of spherical, anisotropic and large Au NSs with this benign approach. The obtained Au NSs were possible to separate small and large Au NSs through centrifugation. Chemistry of pollen extracts played key role on morphology and stability of the Au NSs. The findings, for the first time, is revealing the synthesis of large Au nanorod bundles (>300 nm) along with hexagonal and spherical Au NSs under ambient conditions using pollen grain extracts, whose maturation took 24h.Article Citation - WoS: 6Citation - Scopus: 10Surface Chemistry Dependent Toxicity of Inorganic Nanostructure Glycoconjugates on Bacterial Cells and Cancer Cell Lines(Elsevier, 2023) Sancak, Sedanur; Yazgan, İdris; Bayarslan, Aslı Uğurlu; Ayna, Adnan; Evecen, Senanur; Taşdelen, Zehra; Gümüş, Abdurrahman; Sönmez, Hamide Ayçin; Demir, Mehmet Ali; Demir, Sosin; Bakar, Fatma; Dilek Tepe, HafizeSurface functionalized nanostructures have outstanding potential in biological applications owing to their target-specific design. In this study, we utilized laboratory synthesized carbohydrate-derivatives (i.e., galactose, mannose, lactose, and cellobiose derivatives) for aqueous one-pot synthesis of gold (Au) and silver (Ag) nanostructure glycoconjugates (NSs), and iron metal-organic framework glycoconjugates (FeMOFs). This work aims to test whether differences in the surface chemistry of the inorganic nanostructures play roles in revealing their toxicities towards bacterial cells and cancerous cell lines. As of the first step, biological activity of AuNSs, AgNSs, and FeMOFs were tested against a variety of gram (−) and gram (+) bacterial strains, where AgNSs possessed moderate to high antibacterial activities against all the tested bacterial strains, while AuNSs and FeMOFs showed their bacterial toxicity mostly depending on the strain. Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC) determination studies were performed for the nanostructure glycoconjugates, for which μg/mL MBC values were obtained such as (Cellobiose p-aminobenzoic acid_AgNS) CBpAB_AgNS gave 50 μg/mL MBC value for P.aeruginosa and S.kentucy. The activity of selected sugar ligands and corresponding glycoconjugates were further tested on MDA-MB-231 breast cancer and A549 lung cancer cell lines, where selective anticancer activity was observed depending on the surface chemistry as well. Besides, D-penicillamine was introduced to galectin specific sugar ligand coated AuNS glycoconjugates, which showed very strong anticancer activities even at low doses. Overall, the importance of this work is that the surface chemistry of the inorganic nanostructures can be critical to reveal their toxicity towards bacterial cells and cancerous cell lines.