Chemistry / Kimya

Permanent URI for this collectionhttps://hdl.handle.net/11147/4072

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Author: search.filters.author.Arslan Yıldız, AhuPublisher: search.filters.publisher.Elsevier

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  • Article
    Citation - Scopus: 3
    Development of Chrono-Spectral Gold Nanoparticle Growth Based Plasmonic Biosensor Platform
    (Elsevier, 2024) Sözmen, Alper Baran; Baştanlar, Yalın; Elveren, Beste; Sözmen, Alper Baran; Erdoğan, Duygu; Yıldız, Ümit Hakan; Mezgil, Bahadır; Arslan Yıldız, Ahu; Baştanlar, Yalın; Yıldız, Ümit Hakan; Arslan Yıldız, Ahu; 01. Izmir Institute of Technology; 04.01. Department of Chemistry; 03.04. Department of Computer Engineering; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 04. Faculty of Science
    Plasmonic sensor platforms are designed for rapid, label-free, and real-time detection and they excel as the next generation biosensors. However, current methods such as Surface Plasmon Resonance require expertise and well-equipped laboratory facilities. Simpler methods such as Localized Surface Plasmon Resonance (LSPR) overcome those limitations, though they lack sensitivity. Hence, sensitivity enhancement plays a crucial role in the future of plasmonic sensor platforms. Herein, a refractive index (RI) sensitivity enhancement methodology is reported utilizing growth of gold nanoparticles (GNPs) on solid support and it is backed up with artificial neural network (ANN) analysis. Sensor platform fabrication was initiated with GNP immobilization onto solid support; immobilized GNPs were then used as seeds for chrono-spectral growth, which was carried out using NH2OH at varied incubation times. The response to RI change of the platform was investigated with varied concentrations of sucrose and ethanol. The detection of bacteria E.coli BL21 was carried out for validation as a model microorganism and results showed that detection was possible at 102 CFU/ml. The data acquired by spectrophotometric measurements were analyzed by ANN and bacteria classification with percentage error rates near 0% was achieved. The proposed LSPR-based, label-free sensor application proved that the developed methodology promises utile sensitivity enhancement potential for similar sensor platforms. © 2024 The Author(s)
  • Article
    Citation - WoS: 43
    Citation - Scopus: 46
    Glucuronoxylan-Based Quince Seed Hydrogel: a Promising Scaffold for Tissue Engineering Applications
    (Elsevier, 2021) Güzelgülgen, Meltem; Güzelgülgen, Meltem; Özkendir İnanç, Dilce; Yıldız, Ümit Hakan; Yıldız, Ümit Hakan; Arslan Yıldız, Ahu; Arslan Yıldız, Ahu; 04.01. Department of Chemistry; 01. Izmir Institute of Technology; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 04. Faculty of Science
    Natural gums and mucilages from plant-derived polysaccharides are potential candidates for a tissue-engineering scaffold by their ability of gelation and biocompatibility. Herein, we utilized Glucuron-oxylanbased quince seed hydrogel (QSH) as a scaffold for tissue engineering applications. Optimization of QSH gelation was conducted by varying QSH and crosslinker glutaraldehyde (GTA) concentrations. Structural characterization of QSH was done by Fourier Transform Infrared Spectroscopy (MR). Furthermore, morphological and mechanical investigation of QSH was performed by Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM). The protein adsorption test revealed the suitability of QSH for cell attachment. Biocompatibility of QSH was confirmed by culturing NIH-3T3 mouse fibroblast cells on it. Cell viability and proliferation results revealed that optimum parameters for cell viability were 2 mg mi(-1)of QSH and 0.03 M GTA. SEM and DAPI staining results indicated the formation of spheroids with a diameter of approximately 300 pm. Furthermore, formation of extracellular matrix (ECM) microenvironment was confirmed with the Collagen Type-I staining. Here, it was demonstrated that the fabricated QSH is a promising scaffold for 3D cell culture and tissue engineering applications provided by its highly porous structure, remarkable swelling capacity and high biocompatibility. (C) 2021 Published by Elsevier B.V.