Chemistry / Kimya
Permanent URI for this collectionhttps://hdl.handle.net/11147/4072
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Article Aspergillus Sojae Tarafından Üretilen Poligalakturonazın Kısmi Saflaştırılması için Kromatografik Bir Yaklaşım(Sidas Medya Ajans Tanıtım Danışmanlık Ltd. Şti., 2017) Şen, İlknur; Mata-Gomez, Marco A.; Palomares, Marco Rito; Tarı, Canan; Dinç, MelikeBu çalışmanın amacı, A. sojae mutantından poligalakturonaz üretilmesi ve ham ekstraktın kromatografik yöntemlerle kısmi saflaştırılmasıdır. Peptitlerin konfirmasyonu için ilk basamak olarak, jel içinde sindirilmiş sodyum-dodesil-sülfatpoliakrilamid-jel-elektroforezi (SDS-PAGE) jellerinde matriks-yardımlı lazer desorpsiyon/iyonlaştırmalı-uçuş zamanlıkütle spektrometresi (Maldi-TOF MS) analizi yapılmıştır. Poligalakturonaz üretimi için, katı-faz ve derin fermentasyonlarda üç farklı karbon kaynağı kullanılmıştır. Ham ekstrakt ilk olarak iyon değişim kromatografisi (IEXC) ile saflaştırılmıştır ve ardından bunu boyut eleme kromatografisi izlemiştir. Derin [acı portakal kabuğu, şeker pancarı melası ve (NH4)2SO4] ve katı-faz (buğday kepeği, şeker pancarı ve HCl) fermentasyonlarından elde edilen ham ekstraktlar yüksek seviyede poligalakturonaz enzim aktivitesi (sırasıyla 95.22 and 50.27 U/mL) göstermiştir. IEXC toplanmış fraksiyonunun (180, 200 ve 220 mM tuz fraksiyonları) boyut elemesi, en yüksek verimi (%36) ve saflaştırma katını (2.00) göstermiştir. SDS-PAGE'den elde edilen olası poligalakturonaz bantları jel içinde sindirilmiş ve peptit konfirmasyonu için Maldi-TOF-MS ile analiz edilmiştir.Article Extraction and Characterization of Pectin From Fresh Globe Artichoke and Canned Artichoke Waste(Gıda Teknolojisi Derneği, 2017) Ceylan, Çağatay; Bayraktar, Oğuz; Atçı, Erhan; Sarrafi, ŞahinThe pectin contents of fresh globe artichoke (stem, receptacle, and bract) and waste of artichokecanning industry were investigated. The highest pectin amount was found in the stem part of freshglobe artichoke (6.42%) with the highest amount of anhydrogalacturonic acid (AGA) and anhydrouronicacid (AUA) content. The pectin yields of receptacle and bract parts were found to be 5.31 and 4.55%,respectively. The pectin yield from the industrial waste was the lowest, 4.43%. The highest ash content(5.65 %) along with the lowest anhydrouronic acid amount (73.28%) indicated the lowest purity for theindustrial waste. The degrees of esterification for the pectin obtained from the stem, receptacle andbract parts were 55.26%, 52.26%, and 56.17%, respectively indicating the presence of high methyl-esterified(HM) pectin. The pectin from the industrial waste had the lowest degree of esterification (46.02%). TheFTIR results indicated that acid processing affected the structural properties of pectin from the industrialwaste with higher methoxyl content and esterification degree.Article 2’-Methylklavuzon Causes Lipid-Lowering Effects on A549 Non-Small Cell Lung Cancer Cells and Significant Changes on Dna Structure Evidenced by Fourier Transform Infrared Spectroscopy(Elsevier, 2020) Ceylan, Çağatay; Aksoy, Hatice Nurdan; Çağır, Ali; Çetinkaya, HakkıVarious chemical agents are used in the treatment of Non-Small Cell Lung Cancer (NSCLC). 2?-methylklavuzon was proposed as a potential chemotherapeutic agent in cancer treatment based on its topoisomerase inhibition activity. In this study the cellular effects of 2?-methylklavuzon was evaluated on A549 cancer cells using FTIR spectroscopy. 2?-methylklavuzon induced significant changes on both the whole cell lyophilizates and the lipid extracts of the A549 lung cancer cells. 2?-methylklavuzon caused significant structural changes in A549 cell DNA structure: T, A and G DNA breathing modes are lost after the drug application indicating the loss of topoisomerase activity. The level of transcription and RNA synthesis was enhanced. 2?-methylklavuzon induced single stranded DNA formation evidenced by the increase in the ratio of asymmetric/symmetric phosphate stretching modes. 2?-methylklavuzon induced band shifts only in the asymmetric mode of phosphate bonds not in the symmetrical phosphate bond stretching. 2?-methylklavuzon induced A form of DNA topography. In addition to the changes in the DNA structure and transcription 2?-methylklavuzon also caused lipid-lowering effect in A549 cancer cells. 2?-methylklavuzon suppressed lipid unsaturation, however, it induced formation of lipids with ring structures. 2?-methylklavuzon suppressed phosphate-containing lipids significantly and decreased carbonyl containing lipids and cholesterol slightly. 2?-methylklavuzon caused increases in the hydrocarbon chain length. Overall, 2?-methylklavuzon can be used as a lipid-lowering compound in the treatment of NSCLC and other cancer therapies. © 2020 Elsevier B.V.Article Citation - WoS: 11Citation - Scopus: 9Biophysical Evaluation of Physiological Effects of Gilthead Sea Bream (sparus Aurata) Farming Using Ftir Spectroscopy(Elsevier Ltd., 2014) Ceylan, Çağatay; Tanrıkul, Tansel; Özgener, HüseyinSparus aurata is one of the two most important cultured fish species in the Mediterranean region. The present work investigates the effects of culturing in S. aurata liver tissue at the molecular level using Fourier Transform Infrared (FTIR) spectroscopy. FTIR spectroscopy revealed dramatic differences between the wild and aquacultured fish liver cells, which mainly indicated that the level of glycogen increased in the aquacultured samples and the protein/lipid ratio decreased by 42.29% indicating that triglycerides and cholesterol esters increased and the protein content decreased in the aquacultured samples. The 15.99% increase in the level of unsaturation indicated elevated lipid peroxidation. Structural/organisational changes in the nucleic acids along with increased transcriptional status of the liver tissue cells were observed in the cultured fish tissue. All these results indicated that culturing induces significant changes in fish physiology. In addition FTIR spectroscopy is a promising method to monitor the physiological changes in fish physiology.