Bioengineering / Biyomühendislik

Permanent URI for this collectionhttps://hdl.handle.net/11147/4529

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Institution Author: search.filters.institutionAuthor.Dikici, SerkanScopus Q: search.filters.scopusQuartile.Q2

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  • Article
    Citation - WoS: 14
    Citation - Scopus: 16
    Synergistic Effect of Type and Concentration of Surfactant and Diluting Solvent on the Morphology of Emulsion Templated Matrices Developed as Tissue Engineering Scaffolds
    (Elsevier, 2022) Claeyssens, Frederik; Aldemir Dikici, Betül; Dikici, Serkan
    Emulsion templating is an advantageous route for the fabrication of tissue engineering scaffolds. Emulsions are mostly stabilised using surfactants, and the performances of the surfactants depend on various parameters such as emulsification temperature and the presence of the electrolytes. In this study, we suggest that diluting solvent type also has a dramatic impact on the efficiency of the surfactant and morphology of the polymerised emulsions. For this, morphologies of polycaprolactone methacrylate-based polymerised emulsions, which are designed for tissue engineering applications and in vitro biocompatibilities, were shown by our group, prepared using four different surfactants, and three different solvents were investigated. Results showed that the diluting solvent used in the emulsion composition has a strong impact on the performance of the surfactant and consequently on the morphology of polymerised emulsions. Increasing surfactant concentration and diluting solvent volume have an opposite impact on the characteristics of emulsions. Scaffolds with average pore sizes changing from 10 to 78 μm could be fabricated. Establishing these relations enables us to have control over the overall morphology of polymerised emulsions and precisely engineer them for specific tissue engineering applications by tuning solvent and surfactant type and concentration.
  • Article
    Citation - WoS: 46
    Citation - Scopus: 46
    Thiolene- and Polycaprolactone Methacrylate-Based Polymerized High Internal Phase Emulsion (polyhipe) Scaffolds for Tissue Engineering
    (American Chemical Society, 2022) Aldemir Dikici, Betül; Malayeri, Atra; Sherborne, Colin; Dikici, Serkan; Paterson, Thomas; Dew, Lindsey; Claeyssens, Frederik
    Highly porous emulsion templated polymers (PolyHIPEs) provide a number of potential advantages in the fabrication of scaffolds for tissue engineering and regenerative medicine. Porosity enables cell ingrowth and nutrient diffusion within, as well as waste removal from, the scaffold. The properties offered by emulsion templating alone include the provision of high interconnected porosity, and, in combination with additive manufacturing, the opportunity to introduce controlled multiscale porosity to complex or custom structures. However, the majority of monomer systems reported for PolyHIPE preparation are unsuitable for clinical applications as they are nondegradable. Thiol-ene chemistry is a promising route to produce biodegradable photocurable PolyHIPEs for the fabrication of scaffolds using conventional or additive manufacturing methods; however, relatively little research has been reported on this approach. This study reports the groundwork to fabricate thiol- and polycaprolactone (PCL)-based PolyHIPE materials via a photoinitiated thiolene click reaction. Two different formulations, either three-arm PCL methacrylate (3PCLMA) or four-arm PCL methacrylate (4PCLMA) moieties, were used in the PolyHIPE formulation. Biocompatibility of the PolyHIPEs was investigated using human dermal fibroblasts (HDFs) and human osteosarcoma cell line (MG-63) by DNA quantification assay, and developed PolyHIPEs were shown to be capable of supporting cell attachment and viability.
  • Article
    Citation - WoS: 17
    Citation - Scopus: 17
    Developing Wound Dressings Using 2-Deoxy To Induce Angiogenesis as a Backdoor Route for Stimulating the Production of Vascular Endothelial Growth Factor
    (MDPI Multidisciplinary Digital Publishing Institute, 2021) Dikici, Serkan; Yar, Muhammad; Bullock, Anthony J.; Shepherd, Joanna; Roman, Sabiniano; MacNeil, Sheila
    2-deoxy-D-Ribose (2dDR) was first identified in 1930 in the structure of DNA and discovered as a degradation product of it later when the enzyme thymidine phosphorylase breaks down thymidine into thymine. In 2017, our research group explored the development of wound dressings based on the delivery of this sugar to induce angiogenesis in chronic wounds. In this review, we will survey the small volume of conflicting literature on this and related sugars, some of which are reported to be anti-angiogenic. We review the evidence of 2dDR having the ability to stimulate a range of pro-angiogenic activities in vitro and in a chick pro-angiogenic bioassay and to stimulate new blood vessel formation and wound healing in normal and diabetic rat models. The biological actions of 2dDR were found to be 80 to 100% as effective as VEGF in addition to upregulating the production of VEGF. We then demonstrated the uptake and delivery of the sugar from a range of experimental and commercial dressings. In conclusion, its pro-angiogenic properties combined with its improved stability on storage compared to VEGF, its low cost, and ease of incorporation into a range of established wound dressings make 2dDR an attractive alternative to VEGF for wound dressing development.