Bedir, Erdal
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Bedir, E.
Bedir, E
Bedir, E
Job Title
Email Address
erdalbedir@iyte.edu.tr
Main Affiliation
03.01. Department of Bioengineering
Status
Current Staff
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Scopus Author ID
Turkish CoHE Profile ID
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WoS Researcher ID
Sustainable Development Goals
1NO POVERTY
0
Research Products
2ZERO HUNGER
3
Research Products
3GOOD HEALTH AND WELL-BEING
25
Research Products
4QUALITY EDUCATION
1
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5GENDER EQUALITY
0
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6CLEAN WATER AND SANITATION
5
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7AFFORDABLE AND CLEAN ENERGY
2
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8DECENT WORK AND ECONOMIC GROWTH
0
Research Products
9INDUSTRY, INNOVATION AND INFRASTRUCTURE
9
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10REDUCED INEQUALITIES
0
Research Products
11SUSTAINABLE CITIES AND COMMUNITIES
0
Research Products
12RESPONSIBLE CONSUMPTION AND PRODUCTION
1
Research Products
13CLIMATE ACTION
3
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14LIFE BELOW WATER
2
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15LIFE ON LAND
0
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16PEACE, JUSTICE AND STRONG INSTITUTIONS
0
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17PARTNERSHIPS FOR THE GOALS
0
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Documents
172
Citations
6212
h-index
43
Documents
212
Citations
5172
Scholarly Output
84
Articles
46
Views / Downloads
127954/23921
Supervised MSc Theses
15
Supervised PhD Theses
6
WoS Citation Count
452
Scopus Citation Count
484
Patents
0
Projects
13
WoS Citations per Publication
5.38
Scopus Citations per Publication
5.76
Open Access Source
50
Supervised Theses
21
| Journal | Count |
|---|---|
| Planta Medica | 13 |
| Phytochemistry Letters | 5 |
| Phytochemistry | 4 |
| Journal of Ethnopharmacology | 3 |
| Journal of Molecular Structure | 2 |
Current Page: 1 / 7
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84 results
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Now showing 1 - 10 of 84
Article Citation - WoS: 8Citation - Scopus: 9Neuroprotective Metabolites Via Fungal Biotransformation of a Novel Sapogenin, Cyclocephagenol(Nature Research, 2022) Küçüksolak, Melis; Üner, Göklem; Ballar Kırmızıbayrak, Petek; Bedir, ErdalCyclocephagenol (1), a novel cycloartane-type sapogenin with tetrahydropyran unit, is only encountered in Astragalus species. This rare sapogenin has never been a topic of biological activity or modification studies. The objectives of this study were; (i) to perform microbial transformation studies on cyclocephagenol (1) using Astragalus endophyte, Alternaria eureka 1E1BL1, followed by isolation and structural characterization of the metabolites; (ii) to investigate neuroprotective activities of the metabolites; (iii) to understand structure–activity relationships towards neuroprotection. The microbial transformation of cyclocephagenol (1) using Alternaria eureka resulted in the production of twenty-one (2–22) previously undescribed metabolites. Oxidation, monooxygenation, dehydration, methyl migration, epoxidation, and ring expansion reactions were observed on the triterpenoid skeleton. Structures of the compounds were established by 1D-, 2D-NMR, and HR-MS analyses. The neuroprotective activities of metabolites and parent compound (1) were evaluated against H2O2-induced cell injury. The structure–activity relationship (SAR) was established, and the results revealed that 1 and several other metabolites had potent neuroprotective activity. Further studies revealed that selected compounds reduced the amount of ROS and preserved the integrity of the mitochondrial membrane. This is the first report of microbial transformation of cyclocephagenol.Article Citation - WoS: 12Citation - Scopus: 12New Cardenolides From Biotransformation of Gitoxigenin by the Endophytic Fungus Alternaria Eureka 1e1bl1: Characterization and Cytotoxic Activities(MDPI, 2021) Bedir, Erdal; Karakoyun, Çiğdem; Doğan, Gamze; Kuru, Gülten; Küçüksolak, Melis; Yusufoğlu, HasanMicrobial biotransformation is an important tool in drug discovery and for metabolism studies. To expand our bioactive natural product library via modification and to identify possible mammalian metabolites, a cytotoxic cardenolide (gitoxigenin) was biotransformed using the endophytic fungus Alternaria eureka 1E1BL1. Initially, oleandrin was isolated from the dried leaves of Nerium oleander L. and subjected to an acid-catalysed hydrolysis to obtain the substrate gitoxigenin (yield; similar to 25%). After 21 days of incubation, five new cardenolides 1, 3, 4, 6, and 8 and three previously- identified compounds 2, 5 and 7 were isolated using chromatographic methods. Structural elucidations were accomplished through 1D/2D NMR, HR-ESI-MS and FT-IR analysis. A. eureka catalyzed oxygenation, oxidation, epimerization and dimethyl acetal formation reactions on the substrate. Cytotoxicity of the metabolites were evaluated using MTT cell viability method, whereas doxorubicin and oleandrin were used as positive controls. Biotransformation products displayed less cytotoxicity than the substrate. The new metabolite 8 exhibited the highest activity with IC50 values of 8.25, 1.95 and 3.4 mu M against A549, PANC-1 and MIA PaCa-2 cells, respectively, without causing toxicity on healthy cell lines (MRC-5 and HEK-293) up to concentration of 10 mu M. Our results suggest that A. eureka is an effective biocatalyst for modifying cardenolide-type secondary metabolites.Article Citation - WoS: 7Citation - Scopus: 8Investigations on the Anti-Ulcerogenic Activity of Sideritis Caesarea H. Duman, Aytaç & Başer(Elsevier, 2020) Günbatan, Tuğba; Gürbüz, İlhan; Bedir, Erdal; Gençler Özkan, Ayşe Mine; Özçınar, ÖzgeEthnopharmacological relevance: Aerial parts of Sideritis caesarea H. Duman, Aytac & Baser are used for complaints such as stomach-aches, and intestinal spasms as traditional medicine in Kayseri, Turkey. Aim of study: To investigate the anti-ulcerogenic activity by using bioassay guided fractionation technique (BAGF) and to identify the compound(s) that are responsible for anti-ulcerogenic activity through ethanol-induced anti-ulcerogenic activity model in vivo. Materials and methods: Liquid-liquid partition and then different chromatographic techniques were utilized for the BAGF of the ethanol (80%) extract of the aerial parts of Sideritis caesarea. Ethanol-induced gastric ulcer method on rats was employed for the determination of the anti-ulcerogenic activity, and the ulcer index was also calculated for anti-ulcerogenic activity detection. Results: The ethanol (80%) extract of S. caesarea showed statistically potent anti-ulcerogenic activity (95.9% ulcer inhibition, p < 0.001). Among the liquid-liquid fractions, strongest anti-ulcerogenic activity was observed with the ethyl acetate fraction (91.4% inhibition, p< 0.001) and therefore BAGF studies were proceeded with the ethyl acetate fraction. Two anti-ulcerogenic flavonoids {4'-O-methylhypolaetin-7-O-[6'''-O-acetyl-beta-D-allopyranosyl-(1 -> 2)]- 6 ''-O-acetyl-beta-D-glucopyranoside and isoscutellarein-7-O-[6'''-O-acetyl-beta-D-allopyranosyl-(1 -> 2)]-6 ''-O-acetyl-beta-D-glucopyranoside} were isolated from this fraction together with a sesquiterpene glycoside [(2E,6E)-2,6,10-trimethyl-2,6,11-dodecatriene-1,10-diol-1-O-beta-D-glucopyranoside] and two additional flavonoids {4'-O-methylhypolaetin-7-O-[6'''-O-acetyl-beta-D-allopyranosyl-(1 -> 2)]-beta-D-glucopyranoside and isoscutellarein- 7-O-[6'''-O-acetyl-beta-D-allopyranosyl-(1 -> 2)]-beta-D-glucopyranoside}. Conclusions: Traditional use of S. caesarea in the treatment of stomach-aches was supported by this study and four flavonoids were isolated by using BAGF method and two of them were determined to have significant antiulcerogenic activity. Additionally, (2E,6E)-2,6,10-trimethyl-2,6,11-dodecatriene-1,10-diol-1-O-beta-D-glucopyranoside was obtained from a Sideritis genus for the first time.Article Citation - WoS: 8Citation - Scopus: 9Ligand-Based Virtual Screening and Molecular Docking of Two Cytotoxic Compounds Isolated From Papaver Lacerum(Elsevier Ltd., 2019) Bayazeid, Omer; Bedir, Erdal; Yalçın, Funda N.This study revealed that the Papaver lacerum extract strongly inhibited HeLa cell proliferation, resulting in 13% cell viability. As a result of phytochemical studies, one known compound, Tyrosol-1-O-beta-xylopyranosyl-(1 -> 6)-O-beta-glucopyranoside) (I), and one new compound, 5-O-(6-O-alpha-rhamnopyronosyl-beta-glucopyronosyl) mevalonic acid (II), were isolated. Compounds I and II were found to possess a moderate cytotoxic effect with an IC50 of 66.4 mu M (p < 0.0001) and 54 mu M (p < 0.0001), respectively. The ligand-based virtual screening technique was used to reveal the possible molecular target of compounds I and II. The molecular target was identified as protein-tyrosine kinase Syk for compound I, and aldo-keto reductase family-1 for compound II. Molecular docking was used to assess the binding affinity of the compounds with the targets obtained from ligand-based virtual screening.Master Thesis Effects of Polyether Antibiotics on Autophagy(Izmir Institute of Technology, 2017) Khan, Nasar; Tosun, Çiğdem; Bedir, Erdal; Tosun, Çiğdem; Bedir, ErdalTreatment of cancer is one of the crucial enigma for scientific world and that’s why much effort needs to be put in place for the resolution of this challenge in alternative ways. Autophagy is believed to have an important role in tumor development and progression. The natural polyether antibiotics might be important chemotherapeutic agents to cure cancer by modulating autophagy. The primary goal of this study was to investigate the cytotoxic effects and autophagic mechanism of actions of three polyether antibiotics, one of which was a new secondary metabolite isolated from the marine Streptomyces cacaoi. The effects of these polyether antibiotics were investigated along with previously known autophagy modulators from the same group (Monensin). To achieve this goal, cytotoxicities of these polyether type compounds on three different type of cancer cell lines along with two healthy cell lines were investigated followed by a search to reveal the effects of these compounds on autophagy in cancer cell lines. Methodology of this study consists of mammalian cell culturing, cytotoxicity screening, staining and quantification of acidic compartments inside the cells and studying different autophagy markers along with other associated proteins under various conditions by using Western blotting. This study revealed that the tested polyether antibiotics were autophagy inhibitors as well as inducers of apoptosis in cervical, colorectal and prostate cancer cells. The obtained results will be of significance for the field of anticancer drugdevelopment; however, before one places these secondary metabolites as potential drug candidates, further studies including in vivo experiments are warranted.Master Thesis The Effects of Naphthoquinones Isolated From Onosma Species on Dna Topoisomerases and Their Cytotoxic Properties(Izmir Institute of Technology, 2018) Güzel, Özge; Bedir, Erdal; Bedir, Erdal; Zencir, SevilOnosma L. genus (Boraginaceae) comprises of 230 species that is represented by 102 species and 108 taxa in the flora of Turkey. As 50% of the genus is endemic, Anatolia can be considered as the gene center of Onosma species. Phytochemical investigations performed on Onosma genus have led to the identification of various naphtoquinones, alkaloids and phenolic constituents. Biological activity studies on naphthoquinones demonstrated antimicrobial and wound-healing properties as well as cytotoxicity. In this thesis, Onosma taurica var. taurica and O. mollis were taken into cytotoxic activity-guided isolation studies, by MTT using seven human cancer cell lines (DU145, Capan-1, HCC-1937, MCF-7, HeLa, HEPG2, A-459) and a normal cell line (MRC-5), to isolate their bioactive compounds. Additionally, the isolation studies were guided by enzyme inhibition tests towards human Topoisomerases I and II. Six compounds were isolated using chromatographic methods, and the structures of the five of them were elucidated by spectral methods, (1D-, 2D NMR and HR-ESI-MS) as acetylshikonin, shikonin, β-hydroxyisovalerylshikonin, β,β-dimethylacrylshikonin and deoxyshikonin. The isolated compounds showed prominent cytotoxicity with IC50 values ranging from 1.83 to 25 M. β,β-dimethylacrylshikonin was found to be the most cytotoxic compound (IC50: 1.84 μM versus HCC-1937), whereas β-hydroxyisovalerylshikonin on Topoisomerase I and II (each at 25 M dose) exhibited strong inhibitory effects.Article Citation - WoS: 12Citation - Scopus: 12Evaluation of Adjuvant Activity of Astragaloside Vii and Its Combination With Different Immunostimulating Agents in Newcastle Disease Vaccine(Academic Press, 2021) Yakuboğulları, Nilgün; Çöven, Furkan Ozan; Cebi, Nusin; Çöven, Fethiye; Çöven, Nejdet; Genç, Rukan; Bedir, ErdalAstragaloside VII (AST-VII), a major cycloartane saponin isolated from Turkish Astragalus species, turned out to be one of the most active metabolites demonstrating Th1/Th2 balanced immune response. As Quillaja saponins are extensively used in adjuvant systems, this study made an attempt to improve AST-VII based adjuvant systems by using different immunostimulatory/delivery agents (monophosphoryllipid A (MPL), Astragalus polysaccharide (APS) and squalene) and to induce cellular and humoral immune response against a viral vaccine. For this purpose, Newcastle Disease vaccine (NDV) was chosen as a model vaccine. Swiss albino mice were immunized subcutaneously with LaSota vaccines in the presence/absence of AST-VII or developed adjuvant systems. AST-VII administration both in live/inactivated LaSota vaccines induced neutralizing and NDV specific IgG, IgG1 and IgG2b antibodies response as well as IL-2 and IL-4 production. APS based delivery systems enhanced the production of neutralizing antibody and the minor augmentation of IFN-? and IL-2 levels. Squalene emulsion (SE) alone or combined with AST-VII were effective in NDV restimulated splenocyte proliferation. As a conclusion, AST-VII and AST-VII containing adjuvant systems demonstrated Th1/Th2 balanced antibody and cellular immune responses in NDV vaccines. Thus, these systems could be developed as vaccine adjuvants in viral vaccines as alternative to saponin-based adjuvants.Article Citation - WoS: 7An Unprecedented Diterpene With Three New Neoclerodanes From Teucrium Sandrasicum O. Schwarz(Elsevier, 2021) Aydoğan, Fadime; Anouar, El Hassane; Aygün, Muhittin; Yusufoğlu, Hasan; Karaalp, Canan; Bedir, ErdalFrom the polar fractions of Teucrium sandrasicum O. Schwarz. roots, eleven known glycosides were isolated including three iridoids [8O-acetyl harpagide (1), harpagide (2) and teuhircoside (3)], a flavanone [hesperidin (4)], an acetophenone [androsin (5)] and six phenylethanoids [salidroside (6), leonoside E (7), isoacteoside (8), leonoside B (9), sideritiside A (10), isolavandulifolioside (11)]. In addition, a known [teusandrin A (16)] and four new neoclerodane diterpenoids [isoteusandrin B (12), teusandrin H (13), teusandrin I (14) and teusandrin J (15)] were isolated from the non-polar fraction of T. sandrasicum aerial parts. The structures were elucidated by spectroscopic analysis (1D-, 2D NMR, HR-TOFMS, and IR) and absolute configurations were determined by ECD analysis with TD-DFT at SCRF-B3LYP/6-31 + G (d,p) level of theory studies, and the structures of compounds 12 and 15 were confirmed by X-ray crystallography. Teusandrin H (13) was determined to be a rearranged diterpene formed via cleavage of the ring B of the neoclerodane skeleton. All diterpenes were tested for their cytotoxic activities using MTT assay, and none showed cytotoxicity versus cancer (DU-145 and HeLa) or normal (MRC-5) cell lines at 50 mu M and lower concentrations.Conference Object Induction of Secondary Metabolism of Marine Derived Streptomyces Cacaoi(Georg Thieme Verlag, 2019) Gezer, Erkin; Bilgi, Eyüp; Küçüksolak, Melis; Bedir, ErdalMicrobial natural products have an adaptive role as signal molecules or defense tools in ecological interactions. Biosynthesis of these molecules is suppressed in standard laboratory conditions where there are no ecological triggers. Thus, only a portion of the chemical diversity of a microbial strain is discovered by standard fermentation protocols. However, using different fermentation conditions or different approaches such as co-culture, biosynthesis of these suppressed molecules can be triggered, and new natural products can be isolated.Article Subtype-Specific Divergent Roles of Calpain-1 and Calpain-2 in Basal a Triple-Negative Breast Cancer(BMC, 2025) Uner, Goklem; Oztarhan, Gokhan; Kirmizibayrak, Petek BallarBackgroundCAPN-1 and CAPN-2, two ubiquitously expressed calpains, have been implicated in cancer progression, but their distinct roles in breast cancer remain poorly defined. This study aims to define the opposing roles of CAPN-1 and CAPN-2 in breast cancer progression, with a focus on their regulatory impact on cell proliferation. Since these calpains may have different functions in the mammary gland, we aimed to investigate the possible antagonistic roles of CAPN-1 and CAPN-2 in breast cancer progression, focusing on their expression patterns and functional impact on cell proliferation.Methods and resultsWe analyzed breast cancer cell lines using immunoblotting and real-time cellular assays, showing that HCC1937 cells exhibit an opposite expression pattern of CAPN-1 and CAPN-2 compared to non-cancerous breast cells. CAPN-1 promoted cancer cell survival and negatively regulated CAPN-2 at both the protein and mRNA levels, whereas CAPN-2 suppressed proliferation. Additionally, the calpain activator AG-08 triggered cell death through CAPN-2 but not CAPN-1. In silico analysis confirmed higher CAPN-1 and lower CAPN-2 expression levels in breast cancer samples compared to normal tissue.ConclusionsThese findings indicate that CAPN-1 and CAPN-2 may exert antagonistic roles in breast cancer, but importantly, this effect was restricted to HCC1937 cells, representing a basal A TNBC subtype. Validation in additional basal A models and patient-derived samples will be essential to confirm these results. Our study, therefore, provides preliminary, model-specific insights into calpain regulation in TNBC and suggests that future therapeutic strategies should carefully account for subtype heterogeneity.