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Ünal, Yağmur Ceren
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01. Izmir Institute of Technology
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Current Staff
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Sustainable Development Goals
1NO POVERTY
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2ZERO HUNGER
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3GOOD HEALTH AND WELL-BEING
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4QUALITY EDUCATION
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5GENDER EQUALITY
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6CLEAN WATER AND SANITATION
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7AFFORDABLE AND CLEAN ENERGY
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8DECENT WORK AND ECONOMIC GROWTH
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9INDUSTRY, INNOVATION AND INFRASTRUCTURE
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10REDUCED INEQUALITIES
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11SUSTAINABLE CITIES AND COMMUNITIES
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12RESPONSIBLE CONSUMPTION AND PRODUCTION
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13CLIMATE ACTION
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14LIFE BELOW WATER
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15LIFE ON LAND
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16PEACE, JUSTICE AND STRONG INSTITUTIONS
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Scholarly Output
7
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4
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39560/3253
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1
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WoS Citation Count
46
Scopus Citation Count
51
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WoS Citations per Publication
6.57
Scopus Citations per Publication
7.29
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5
Supervised Theses
1
| Journal | Count |
|---|---|
| 2020 Medical Technologies Congress (Tiptekno) | 1 |
| Biochimica et Biophysica Acta - Molecular Cell Research | 1 |
| Biotechnology and Bioengineering | 1 |
| Journal of Drug Delivery Science and Technology | 1 |
| Tissue Barriers | 1 |
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Now showing 1 - 7 of 7
Article Citation - WoS: 8Citation - Scopus: 8The Role of Connexins in Breast Cancer: From Misregulated Cell Communication To Aberrant Intracellular Signaling(Taylor & Francis, 2022) Yavuz, Büşra; Ünal, Yağmur Ceren; Özçivici, Engin; Meşe Özçivici, Gülistan; 03.01. Department of Bioengineering; 01. Izmir Institute of Technology; 04.03. Department of Molecular Biology and Genetics; 03. Faculty of Engineering; 04. Faculty of ScienceIn spite of clinical advancements and improved diagnostic techniques, breast cancers are the leading cause of cancer-associated deaths in women worldwide. Although 70% of early breast cancers can be cured, there are no efficient therapies against metastatic breast cancers. Several factors including connexins and gap junctions play roles in breast tumorigenesis. Connexins are critical for cellular processes as a linkage between connexin mutations and hereditary disorders demonstrated their importance for tissue homeostasis. Further, alterations in their expression, localization and channel activities were observed in many cancers including breast cancer. Both channel-dependent and independent functions of connexins were reported in initiation and progression of cancers. Unlike initial reports suggesting tumor suppressor functions, connexins and gap junctions have stage, context and isoform dependent effects in breast cancers similar to other cancers. In this review, we tried to describe the current understanding of connexins in tumorigenesis specifically in breast cancers.Article Citation - WoS: 22Citation - Scopus: 24Scaffold-Free Biofabrication of Adipocyte Structures With Magnetic Levitation(John Wiley and Sons Inc., 2021) Yalçın Özuysal, Özden; Meşe Özçivici, Gülistan; Fıratlıgil Yıldırır, Burcu; Ünal, Yağmur Ceren; Özçivici, Engin; Sarıgil, Öykü; Anıl İnevi, Müge; Tekin, Hüseyin Cumhur; Yalçın Özuysal, Özden; Özçivici, Engin; Meşe, Gülistan; Sarıgil, Öykü; Özçivici, Engin; Anıl İnevi, Müge; Meşe Özçivici, Gülistan; 03.01. Department of Bioengineering; 01. Izmir Institute of Technology; 04.03. Department of Molecular Biology and Genetics; 03. Faculty of Engineering; 04. Faculty of ScienceTissue engineering research aims to repair the form and/or function of impaired tissues. Tissue engineering studies mostly rely on scaffold-based techniques. However, these techniques have certain challenges, such as the selection of proper scaffold material, including mechanical properties, sterilization, and fabrication processes. As an alternative, we propose a novel scaffold-free adipose tissue biofabrication technique based on magnetic levitation. In this study, a label-free magnetic levitation technique was used to form three-dimensional (3D) scaffold-free adipocyte structures with various fabrication strategies in a microcapillary-based setup. Adipogenic-differentiated 7F2 cells and growth D1 ORL UVA stem cells were used as model cells. The morphological properties of the 3D structures of single and cocultured cells were analyzed. The developed procedure leads to the formation of different patterns of single and cocultured adipocytes without a scaffold. Our results indicated that adipocytes formed loose structures while growth cells were tightly packed during 3D culture in the magnetic levitation platform. This system has potential for ex vivo modeling of adipose tissue for drug testing and transplantation applications for cell therapy in soft tissue damage. Also, it will be possible to extend this technique to other cell and tissue types.Article Citation - WoS: 16Citation - Scopus: 18Connexin 32 Induces Pro-Tumorigenic Features in Mcf10a Normal Breast Cells and Mda-Mb Metastatic Breast Cancer Cells(Elsevier, 2020) Meşe Özçivici, Gülistan; Ünal, Yağmur Ceren; Yücel, Simge; Yalçın Özuysal, Özden; Vural, Zehra; Turan, Fatma Başak; Meşe, Gülistan; 01. Izmir Institute of Technology; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of ScienceConnexins (Cx), the basic subunit of gap junctions, play important roles in cell homeostasis, and their abnormal expression and function are associated with human hereditary diseases and cancers. In tumorigenesis, connexins were observed to have both anti-tumorigenic and pro-tumorigenic roles in a context- and stage-dependent manner. Initially, Cx26 and Cx43 were thought to be the only connexins involved in normal breast homeostasis and breast cancer. Later on, association of Cx32 expression with lymph node metastasis of breast cancer and subsequent demonstration of its expression in normal breast tissue suggested that Cx32 contributes to breast tissue homeostasis. Here, we aimed to determine the effects of Cx32 on normal breast cells, MCF10A, and on breast cancer cells, MDA-MB-231. Cx32 overexpression had profound effects on MCF10A cells, decreasing cell proliferation by increasing the doubling time of MCF10A. Furthermore, MCF10A cells acquired mesenchymal-like appearance upon Cx32 expression and had increased migration capacity and expression of both E-cadherin and vimentin. In contrast, Cx32 overexpression altered the EMT markers of MDA-MB-231 by increasing the expression of mesenchymal markers, such as slug and vimentin, and decreasing E-cadherin expression without affecting their proliferation and morphology. Our results indicate, for the first time in the literature, that Cx32 has tumor-promoting roles in MCF10A and MDA-MB-231 cells.Conference Object Assessment of Cell Cycle and Viability of Magnetic Levitation Assembled Cellular Structures(IEEE, 2020) Meşe Özçivici, Gülistan; Tekin, Hüseyin Cumhur; Yaman, Sena; Anıl İnevi, Müge; Ünal, Yağmur Ceren; Meşe, Gülistan; 03.01. Department of Bioengineering; 01. Izmir Institute of Technology; 04.03. Department of Molecular Biology and Genetics; 03. Faculty of Engineering; 04. Faculty of ScienceLabel-free magnetic levitation is one of the most recent Earth-based in vitro techniques that simulate the microgravity. This technique offers a great opportunity to biofabricate scaffold-free 3-dimensional (3D) structures and to study the effects of microgravity on these structures. In this study, self-assembled 3D living structures were fabricated in a paramagnetic medium by magnetic levitation technique and effects of the technique on cellular health was assessed. This magnetic force-assisted assembly system applied here offers broad applications in several fields, such as space biotechnology and bottom-up tissue engineering.Master Thesis Time Dependent Expression and Localization of Connexin 32: Implication in Epithelial To Mesenchymal Transition of Mammary Epithelial Mcf10a and Triple Negative Breast Cancer Mda Mb 231 Cells(01. Izmir Institute of Technology, 2020) Ünal, Yağmur Ceren; Meşe Özçivici, Gülistan; Meşe Özçivici, Gülistan; 01. Izmir Institute of Technology; 04.03. Department of Molecular Biology and Genetics; 04. Faculty of ScienceBreast cancer is the most frequent and the second leading cause of cancer-related deaths among women worldwide. Epithelial to mesenchymal transition (EMT) is critical driving force in metastasis. Connexins as a basic subunit of gap junctions indicate critical roles in regulation of EMT. In addition to Cx26 and Cx43, Cx32 is associated with breast cancer and elevated levels of Cx32 has been reported in lymph node metastasis compared to primary breast cancer while the role of Cx32 in breast cancer is still elusive. Here we aimed to shed light on the effect of Cx32 on breast cancer. Our study suggested that Cx32 acquired mesenchymal morphology and decreased proliferation in MCF10A cells but not in MDA MB 231 cells. To further elucidate whether Cx32 indicate these changes through EMT, EMT markers were examined and subsequently it was revealed that Cx32 expression was strongly correlated with increased E-cadherin and Vimentin in MCF10A cells while decreased E-cadherin and Snail in MDA MB 231 cells. Importantly majority of Cx32 did not localize to the plasma membrane and indicated dynamic changes in a day dependent manner in both MCF10A and MDA MB 231 cells. Moreover, day dependent expression and localization of Cx32 revealed strong correlation with Zeb2 expression in MCF10A cells. In conclusion, Cx32 indicated differential effects in regulation of EMT between MCF10A and MDA MB 231 cells. It was the first time that the role of Cx32 on EMT was investigated in breast cancer and differential localization of Cx32 was identified.Publication Connexin26 Mutasyonlarının Neden Olduğu Kid Sendromunda Gözlenen Epidermal Fenotiplerin Oluşmasında Nf-kb Yolağının Rolünün Araştırılması(2024) Yavuz, Büşra; Ünal, Yağmur Ceren; Yıldırım, Meryem Azra; İnal, Ece; Ünal, Yağmur Ceren; Elgin, Resul Gökberk; Öz, Sercan; 01. Izmir Institute of TechnologyConnexinlerin oluşturduğu gap junctionlar ve yarım kanallar insan vücudunda deri gibi bir çok doku ve organın normal olarak faaliyetlerini devam ettirmesinde önemli görevler üstlenirler. Connexin26 (Cx26) mutasyonları hem sendromik olmayan sağırlığa hem de keratitis-ichthyosis-deafness (KID) sendromu gibi deri hastalıklarıyla bağlantılı sendromik sağırlığa neden olmaktadır. Sendromik sağırlığa neden olan mutasyonlar Cx26?nın yeni fonksiyonlar kazanmasına neden olmakta, ancak bunun epidermal hücrelerin fizyolojisini nasıl etkilediği tam olarak bilinmemektedir. Bu projede, KID sendromuna neden olan Cx26-G45E ve Cx26-D50Y mutasyonları ile Cx26-WT içeren HaCaT keratinosit hücrelerinde mutasyonların NF-?B sinyal yolağı moleküllerinin ifadelerine ve NF-?B yolak aktivitesindeki değişimlere etkileri araştırılmıştır. Ayrıca, hücre içi ve hücre dışı kalsiyum konsantrasyonlarının azaltılmasının bu mekanizmalara etkileri sırasıyla BAPTA-AM ve BAPTA kalsiyum çekici molekülleri uygulaması sonucunda incelenmiştir. Son olarak, hücrelerde NF-?B yolağının inhibisyonunun hücrelerin canlılık/çoğalmaları, hücre döngüsü, hücre ölümü ve hücrelerin farklılaşmalarına etkileri tespit edilmiştir. Bu çalışmalar sonucunda, Cx26-D50Y içeren hücrelerde RelB ve c-Rel mRNA ifadesi azalmış, buna karşılık NF-?B yolak aktivitesi artmıştır. Buna karşılık, Cx26-G45E mutasyonunda yolak moleküllerinin ifadelerinde farklılık gözlenmezken yolak aktivitesi azalmıştır. Ayrıca, p65?in Cx26-G45E hücrelerinde Cx26-WT ve Cx26-D50Y?ye kıyasla hem sitoplazmada hem de çekirdekte bulunduğu ve c-Rel proteinin her iki mutasyonda çekirdekte lokalize olduğu tespit edilmiştir. Hücre içi kalsiyumun azalmasının Cx26 overekspresyonu olan hücrelerde RelB?nin çekirdeğe geçişini artırdığı gözlenmiştir. Ayrıyeten, hücre dışı kalsiyumun Cx26-D50Y hücrelerinde p65 ve c-Rel mRNA ifadesini azalttığı tespit edilmiştir. NF-?B inhibisyonu MTT çalışmalarında hücre canlılığını/çoğalmasını azaltmış, ancak, hücre proliferasyonu, döngüsü ve apoptozunu etkilememiştir. Buna karşılık, Cx26-G45E içeren hücrelerde farklılaşma belirteci cytokeratin 10 ve involucrinin arttığı ve cytokeratin 10?daki artışın NF-?B sinyal yolağının inhibisyonu ile azaldığı gözlenmiştir. Sonuç olarak, Cx26 KID sendromu mutasyonlarının NF-?B sinyal yolağına farklı şekillerde etkilerinin olduğu ve bunun hastalarda mutasyona özgü gözlenen epidermal fenotiplerin gelişmesinde rol oynayabileceği değerlendirilmiştir.Article Citation - Scopus: 1A Diaminoethane Motif Bearing Low Molecular Weight Polymer as a New Nucleic Acid Delivery Agent(Elsevier, 2021) Bulmuş Zareie, Volga; Ünal, Yağmur Ceren; Meşe Özçivici, Gülistan; Bulmuş, Volga; 01. Izmir Institute of Technology; 04.03. Department of Molecular Biology and Genetics; 03.01. Department of Bioengineering; 03. Faculty of Engineering; 04. Faculty of ScienceAmong polymer-based gene delivery systems, poly(ethylene imine) (PEI) stands out as an effective polycation. However, the toxic effects of PEI especially at higher molecular weights limit its usage. Although the effects of PEI's architecture and molecular weight on gene delivery is controversial in literature, low molecular weight PEI appears to be efficient at transfection while having lower toxicity. Herein, as an alternative to low molecular weight, linear PEI, a methacrylate polymer bearing diamimoethane motifs, poly(2-((2-aminoethyl)amino)ethyl methacrylate) (P(AEAEMA)), was evaluated in vitro as a new nucleic acid delivery agent. P(AEAEMA) (8 kDa) showed low toxicity on Skov-3-luc and NIH/3T3 cell lines at polymer concentrations where PEI (8 kDa) was highly toxic. P(AEAEMA) could efficiently form complexes with siRNA at an N/P ratio of 2 as shown by gel electrophoresis. The diameter of P(AEAEMA)-siRNA complexes was found to be significantly lower than PEIsiRNA complexes almost at all tested N/P ratios. P(AEAEMA) could improve the stability of siRNA in serum containing media by protecting the siRNA against serum nucleases. siRNA and pDNA transfection efficiency of P (AEAEMA) on luciferase expressing Skov-3-luc cell line and HEK 293T cell line, respectively was found to be comparable to well-known nucleic acid carrier, PEI. The transfection efficiency of both P(AEAEMA) and PEI was found to be cell-type-dependent. None of the polymers were able to transfect MDA-MB-231 cells with siRNA or pDNA.