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Yurttaş, Betül
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01. Izmir Institute of Technology
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2
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2.00
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| Materials Today Communications | 1 |
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Master Thesis Development of Conductive Oxide Based Thin Film Modified Electrodes and Biosensors Applications(Izmir Institute of Technology, 2021) Özyüzer, Lütfi; Yurttaş, Betül; Özyüzer, Lütfi; Erdem Gürsan, Kadriye Arzum; 01. Izmir Institute of Technology; 04.05. Department of Pyhsics; 04. Faculty of ScienceFrom the first biosensor produced in 1956 to the present day, biosensors have been highly developed and diversified. In biosensor manufacturing, thin films have become a rapidly emerging field. Depending on the thin film material used, thin films have many advantageous properties for biosensors, such as high surface-to-volume ratio, conductivity, stability, specificity, biocompatibility, and good electrocatalytic activity. Dopamine is a neurotransmitter that has a significant impact on the emergence and treatment of certain diseases such as Alzheimer's and Parkinson's diseases. Dopamine monitoring is important for the prevention of these diseases, and it is a favorable option to use biosensors, which are useful and practical tools, instead of time-consuming and expensive conventional methods. For this purpose, in this thesis, a non-enzymatic electrochemical biosensor based on thin film electrodes was developed for monitoring dopamine levels. The electrodes were developed by deposition of Zn2SnO4 (ZTO) thin film on ITO thin film substrate by DC magnetron sputtering technique. The properties of the electrodes were determined by thickness, optical transmittance, XRD and SEM analysis. Electrochemical analysis, namely CV, EIS and DPV measurements, were performed before and after the electrodes were sonicated and modified with APTES before their application to the voltammetric detection of dopamine. In addition, electrochemical measurements were performed before/after sonication, APTES modification. Dopamine was detected by a voltammetric method using DPV technique. Furthermore, experiments in the presence of interferents such as ascorbic acid (AA), uric acid (UA) etc. showed that the thin film electrodes can be successfully applied for voltammetric determination of dopamine. As a result, the biosensor technology developed in this study has the potential to be wearable in the future, enabling non-invasive monitoring of dopamine levels in body fluids such as saliva, tears and sweat.Article Citation - WoS: 4Citation - Scopus: 4Development of Single-Use Thin Film Electrodes Based on Zn2sno4 on In2o3:sno2 Substrates With Their Biosensing Applications(Elsevier, 2022) Yurttaş, Betül; Maral, Meltem; Yurttaş, Betül; Özyüzer, Lütfi; 01. Izmir Institute of Technology; 04.05. Department of Pyhsics; 04. Faculty of ScienceDopamine (DA) has a significant impact on the emergence and treatment of certain diseases (e.g., Alzheimer's and Parkinson's diseases). Therefore, monitoring of DA is important, and using biosensors is a favorable option instead of time-consuming and expensive conventional methods. In biosensor manufacturing, thin films have become a rapidly emerging field. In this study, a non-enzymatic electrochemical biosensor based on thin film electrodes is developed for monitoring DA levels. The thin film electrodes (ZTO/ITO) are developed by deposition of Zn2SnO4 (ZTO) on In2O3:SnO2 (ITO) substrates by magnetron sputtering. 3-aminopropyltriethoxysilane (APTES) is used to modify the surface of these electrodes. Physical, optical, and structural properties of the electrodes are determined by applying surface profilometry, UV–VIS–NIR spectrophotometry, X-ray diffraction (XRD), and scanning electron microscopy (SEM) measurements. According to these measurements, it has been observed that the ZTO/ITO combination has a higher optical transmission value than the bare ITO, depending on the deposition time and the oxygen concentration used during ZTO deposition. In addition, the ITO thin film has a crystalline structure, while the ZTO thin film has an amorphous structure and both thin films have a good surface morphology. As electrochemical analysis, cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV) measurements are performed. As a result of CV and EIS measurements, a remarkable change (63.54%) was observed after applying APTES modification onto the surface of ZTO/ITO electrode, and the ones obtained by DPV showed successful detection of DA by APTES modified ZTO/ITO. In addition, the experiments in the presence of interferences such as ascorbic acid (AA), uric acid (UA), bovine serum albumin (BSA), and fish sperm double-stranded DNA (fsDNA) show that the electrodes can be successfully applied for voltammetric determination of DA. The detection limit of DA was estimated to be 0.013 µM in the range of DA between 0.1 and 1 µM, and sensitivity was calculated and found to be 11.057 μA μg−1 mL cm−2, which means ZTO/ITO electrodes have a good sensitivity.