Quantification of Staphylococcus Aureus in White Cheese by the Improved Dna Extraction Strategy Combined With Taqman and Lna Probe-Based Qpcr
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Date
2014
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier Ltd.
Open Access Color
BRONZE
Green Open Access
Yes
OpenAIRE Downloads
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Publicly Funded
No
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Impulse
Average
Influence
Average
Popularity
Top 10%
Abstract
Four different bacterial DNA extraction strategies and two different qPCR probe chemistries were studied for detection of Stapylococcus aureus from white cheeses. Method employing trypsin treatment followed by a commercial kit application and TaqMan probe-based qPCR was the most sensitive one detecting higher counts than standards in naturally contaminated samples.
Description
Keywords
Cheese, LNA, QPCR, Staphylococcus aureus, TaqMan, DNA, Bacterial, Staphylococcus aureus, TaqMan, Real-Time Polymerase Chain Reaction, Sensitivity and Specificity, Bacterial Load, QPCR, LNA, Cheese
Fields of Science
0301 basic medicine, 0303 health sciences, 03 medical and health sciences
Citation
Kadiroǧlu, P., Korel, F., and Ceylan, C. (2014). Quantification of Staphylococcus aureus in white cheese by the improved DNA extraction strategy combined with TaqMan and LNA probe-based qPCR. Journal of Microbiological Methods, 105, 92-97. doi:10.1016/j.mimet.2014.06.022
WoS Q
Q3
Scopus Q
Q3
OpenCitations Citation Count
10
Source
Journal of Microbiological Methods
Volume
105
Issue
Start Page
92
End Page
97
PlumX Metrics
Citations
CrossRef : 10
Scopus : 9
PubMed : 1
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Mendeley Readers : 25
SCOPUS™ Citations
9
checked on Apr 27, 2026
Web of Science™ Citations
11
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Page Views
876
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Downloads
711
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