Biofunctional Quantum Dots as Fluorescence Probe for Cell-Specific Targeting
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Abstract
We describe here the synthesis, characterization, bioconjugation, and application of water-soluble thioglycolic acid TGA-capped CdTe/CdS quantum dots (TGA-QDs) for targeted cellular imaging. Anti-human epidermal growth factor receptor 2 (HER2) antibodies were conjugated to TGA-QDs to target HER2-overexpressing cancer cells. TGA-QDs and TGA-QDs/anti-HER2 bioconjugates were characterized by fluorescence and UV-Vis spectroscopy, X-ray diffraction (XRD), hydrodynamic sizing, electron microscopy, and gel electrophoresis. TGA-QDs and TGA-QDs/anti-HER2 were incubated with cells to examine cytotoxicity, targeting efficiency, and cellular localization. The cytotoxicity of particles was measured using an MTT assay and the no observable adverse effect concentration (NOAEC), 50% inhibitory concentration (IC50), and total lethal concentration (TLC) were calculated. To evaluate localization and targeting efficiency of TGA-QDs with or without antibodies, fluorescence microscopy and flow cytometry were performed. Our results indicate that antibody-conjugated TGA-QDs are well-suited for targeted cellular imaging studies.
Description
Keywords
Bioconjugation, Cell specific targeting, Imaging, Quantum dots, Antibodies, thioglycolic acid, Light, Dewey Decimal Classification::500 | Naturwissenschaften::570 | Biowissenschaften, Biologie, Receptor, ErbB-2, Imaging techniques, animal cell, fluorescence microscopy, Imaging, Mice, Colloid and Surface Chemistry, X-Ray Diffraction, tellurium, cancer diagnosis, Cadmium Compounds, Semiconductor quantum dots, Lethal concentration, cellular distribution, target cell, receptor antibody, cancer cell, Fluorescence microscopy, Ultraviolet visible spectroscopy, Cell Death, Quantum dots, drug cytotoxicity, quantum dot, Surfaces and Interfaces, cell assay, unclassified drug, Cell specific targeting, cell surface, Fluorescence probes, priority journal, Thioglycolates, drug concentration, Tellurium, Inhibitory concentration, Biotechnology, Electrophoresis, Receptor, erbB-2, drug conjugation, X ray diffraction, Cellular localization, cadmium, drug design, Cell Survival, Cells, cell specificity, ultraviolet spectroscopy, Fluorescence, Antibodies, 3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide, Epidermal growth factor receptor 2, fluorescence imaging, Cell Line, Tumor, Quantum Dots, Bio-conjugation, Animals, Humans, controlled study, Physical and Theoretical Chemistry, epidermal growth factor receptor 2 antibody, protein expression, mouse, Fluorescent Dyes, nonhuman, electron microscopy, total lethal concentration, flow cytometry, human cell, Cell Membrane, Bioconjugation, Reproducibility of Results, no observable adverse effect concentration, Gel electrophoresis, Anti-HER2, internalization, lung cancer, Microscopy, Fluorescence, sulfur, hydrodynamics, drug solubility, NIH 3T3 Cells, drug synthesis, epidermal growth factor receptor 2, cell labeling
Fields of Science
02 engineering and technology, 0210 nano-technology
Citation
Ağ, D., Bongartz, R., Eral Doğan, L., Seleci, M., Walter, J. G., Odacı Demirkol, D., Stahl, F., Özçelik, S., Timur, S., and Scheper, T. (2014). Biofunctional quantum dots as fluorescence probe for cell-specific targeting. Colloids and Surfaces B: Biointerfaces, 114, 96-103. doi:10.1016/j.colsurfb.2013.09.033
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OpenCitations Citation Count
51
Volume
114
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Start Page
96
End Page
103
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