Rflp of 16s-Its Rdna Region To Differentiate Lactobacilli at Species Level
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BRONZE
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Yes
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Abstract
The 16S-ITS (internal transcribed spacer) region of the rrn operon was amplified by polymerase chain reaction (PCR). The amplification products were analysed by restriction fragment length polymorphism (RFLP) using a set of restriction enzymes, AluI, HaeIII, and TaqI. Restriction pattern analyses revealed that TaqI restriction enzyme could clearly differentiate the nine reference strains of Lactobacillus used in the study.
Description
Keywords
Lactic acid bacteria, Similarity analysis, Restriction fragment length polymorphism, Lactobacillus, Similarity analysis, Lactobacillus, Lactic acid bacteria, Restriction fragment length polymorphism
Fields of Science
0301 basic medicine, 0303 health sciences, 03 medical and health sciences
Citation
Yavuz, E., Güneş, H., Bulut, Ç., Harsa, H. Ş., and Yenidünya, A. F. (2004). RFLP of 16S-ITS rDNA region to differentiate Lactobacilli at species level. World Journal of Microbiology and Biotechnology, 20(6), 535-537. doi:10.1023/B:WIBI.0000043151.09366.d7
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OpenCitations Citation Count
15
Volume
20
Issue
6
Start Page
535
End Page
537
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