Inhibition of Catechol-O (comt) by Some Plant-Derived Alkaloids and Phenolics
| dc.contributor.author | Yalçın, Dilek | |
| dc.contributor.author | Bayraktar, Oğuz | |
| dc.coverage.doi | 10.1016/j.molcatb.2009.04.014 | |
| dc.date.accessioned | 2017-01-04T13:39:40Z | |
| dc.date.available | 2017-01-04T13:39:40Z | |
| dc.date.issued | 2010 | |
| dc.description.abstract | In this study, as an alternative to the medicines, natural compounds extracted from plant species (Peganum harmala, Cistus parviflorus and Vitex agnus-cactus) were investigated in order to inhibit the catechol-O-metyhltranferase (COMT) activity. In fluorometric enzyme assay, S-adenosylmethionine (SAM) and aesculetin (ES) were used as methyl donor and acceptor substrates, respectively. Their Km values were determined as 3.5 ± 0.3 μM and 6.4 ± 0.4 μM in absence of inhibitor. Inhibition performances of the plant-derived polyphenolics and alkaloids were determined. Inhibitory effect of alkaloids extracted from P. harmala seeds was found the highest among the plant extracts; however, it was lower than that of 3,5-DNC. In case of inhibition mechanism, mixed type inhibition was observed for alkaloid extract whereas uncompetitive inhibition was observed for 3,5-DNC. In case of polyphenolic extracts obtained from C. parviflorus and V. agnus-cactus leaves, mechanism were also explained as mixed type inhibition and their αKi values were calculated as 1.99 ± 0.35 μg/ml and 9.48 ± 0.58 μg/ml, respectively. © 2009 Elsevier B.V. All rights reserved. | en_US |
| dc.description.sponsorship | Turkish Scientific Research Council under Project MAG-107M307; Izmir Institute of Technology under Project 2007IYTE04 | en_US |
| dc.identifier.citation | Yalçın, D., and Bayraktar, O. (2010). Inhibition of catechol-O-methyltransferase (COMT) by some plant-derived alkaloids and phenolics. Journal of Molecular Catalysis B: Enzymatic, 64(3-4), 162-166. doi:10.1016/j.molcatb.2009.04.014 | en_US |
| dc.identifier.doi | 10.1016/j.molcatb.2009.04.014 | en_US |
| dc.identifier.doi | 10.1016/j.molcatb.2009.04.014 | |
| dc.identifier.issn | 1381-1177 | |
| dc.identifier.scopus | 2-s2.0-77951974399 | |
| dc.identifier.uri | http://doi.org/10.1016/j.molcatb.2009.04.014 | |
| dc.identifier.uri | https://hdl.handle.net/11147/2719 | |
| dc.language.iso | en | en_US |
| dc.publisher | Elsevier Ltd. | en_US |
| dc.relation.ispartof | Journal of Molecular Catalysis B: Enzymatic | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.subject | Phenols | en_US |
| dc.subject | Alkaloids | en_US |
| dc.subject | COMT | en_US |
| dc.subject | Inhibition | en_US |
| dc.subject | Parkinson's disease | en_US |
| dc.title | Inhibition of Catechol-O (comt) by Some Plant-Derived Alkaloids and Phenolics | en_US |
| dc.type | Article | en_US |
| dspace.entity.type | Publication | |
| gdc.author.institutional | Yalçın, Dilek | |
| gdc.author.institutional | Bayraktar, Oğuz | |
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| gdc.coar.type | text::journal::journal article | |
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| gdc.description.department | İzmir Institute of Technology. Chemical Engineering | en_US |
| gdc.description.endpage | 166 | en_US |
| gdc.description.issue | 3-4 | en_US |
| gdc.description.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| gdc.description.scopusquality | N/A | |
| gdc.description.startpage | 162 | en_US |
| gdc.description.volume | 64 | en_US |
| gdc.identifier.openalex | W2042170210 | |
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| gdc.oaire.keywords | Alkaloids | |
| gdc.oaire.keywords | Phenols | |
| gdc.oaire.keywords | Parkinson's disease | |
| gdc.oaire.keywords | COMT | |
| gdc.oaire.keywords | Inhibition | |
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| gdc.oaire.sciencefields | 0301 basic medicine | |
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| gdc.oaire.sciencefields | 03 medical and health sciences | |
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