Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik

Permanent URI for this collectionhttps://hdl.handle.net/11147/9

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Author: search.filters.author.Bulut, ÇisemInstitution Author: search.filters.institutionAuthor.Yavuz, Elif

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Now showing 1 - 2 of 2
  • Article
    Citation - WoS: 17
    Citation - Scopus: 20
    Rflp of 16s-Its Rdna Region To Differentiate Lactobacilli at Species Level
    (Springer Verlag, 2004) Yavuz, Elif; Güneş, Hatice; Bulut, Çisem; Harsa, Hayriye Şebnem; Yenidünya, Ali Fazıl
    The 16S-ITS (internal transcribed spacer) region of the rrn operon was amplified by polymerase chain reaction (PCR). The amplification products were analysed by restriction fragment length polymorphism (RFLP) using a set of restriction enzymes, AluI, HaeIII, and TaqI. Restriction pattern analyses revealed that TaqI restriction enzyme could clearly differentiate the nine reference strains of Lactobacillus used in the study.
  • Article
    Citation - WoS: 3
    Citation - Scopus: 3
    Optimization of Pulsed Field Gel Electrophoresis (pfge) Conditions for Thermophilic Bacilli
    (Springer Verlag, 2004) Yavuz, Elif; Güneş, Hatice; Harsa, Hayriye Şebnem; Bulut, Çisem; Yenidünya, Ali Fazıl
    Although thermophilic members of the genus Bacillus are known to tolerate extreme environmental conditions, they appeared to be readily damaged upon mechanical manipulations. This fact was evidenced in genotyping of some strains of thermophilic Bacillus by pulsed field gel electrophoresis (PFGE). Consequently, a new procedure for the preparation of agarose plugs was developed. The procedure produced interpretable genomic DNA restriction profiles.