Bioengineering / Biyomühendislik

Permanent URI for this collectionhttps://hdl.handle.net/11147/4529

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End date: 2019Publisher: search.filters.publisher.Elsevier

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Now showing 1 - 9 of 9
  • Conference Object
    Citation - WoS: 1
    Kinetic and Thermodynamic Properties of Crude and Three-Phase Partitioned Polygalacturonase From Aspergillus Sojae
    (Elsevier, 2007) Doğan, Nergiz; Tari, Canan; Göğüş, Nihan
    Commercial preparations of pectinolytic enzymes derived from fungi are well known to have high biotechnological value in the industry. Since potential applications of pectinases in vari- ous fields of food, paper, textile and waste water treatment are increasing, it is important to understand the properties of these enzymes for efficient and effective usage.
  • Conference Object
    Effect of Symbiotic Relationship of Lactobacillus Butgaricus 77 and Streptococcus Thermophilus 9512 on Betagalactosidase and Lactic Acid Production
    (Elsevier, 2007) Üstok, Fatma Işık; Tari, Canan; Harsa, Şebnem
    Enzyme production which is a growing field of biotechnology has an annual world sale close to billion dollars. Therefore isolation of new strains producing novel enzymes is important for industrial enzyme production.
  • Conference Object
    Citation - WoS: 1
    Optimization of Exo-Polygalacturonase Production From Orange Peel by Aspergillus Sojae
    (Elsevier, 2010) Büyükkileci, Ali Oğuz; Tarı, Canan; Fernandez-Lahore, H. M.; Gençkal Demir, Hande; Göğüş, Nihan
    Pectinases catalyze the degradation of pectic substances, thus they are used extensively in fruit juice and wine industry to facil-itate extraction and clarification. Aspergillus species, in particular Aspergillus niger, have long been utilized for production of pectinases. Previous studies of our group showed that A. sojae has a potential to produce enhanced amount of polygalacturonase, which is one of the pectic enzymes, in both submerged and solid-state cultures (Gogus et al., 2006; Tari et al., 2007). In this study, several agricultural products were screened in an effort to find a cheap and abundant substrate for submerged polygalacturonase production using a UV-mutated A. sojae strain. Medium composition was optimized to further enhance the enzyme level. Experiments were designed and analyzed statistically using the trial version of the statistical software, Design Expert.
  • Conference Object
    Bioethanol Production From Low Cost Agro-Industrial Waste Products
    (Elsevier, 2012) Evcan, Ezgi; Tarı, Canan; Özen, Banu
    In recent years, the rapid increase in environmental problems, greenhouse gas emissions, fuel prices and the unlimited consumption of limited fossil fuel stocks made people search for some alternative energy sources. Bioethanol is one of the most popular alternative source with its many beneficial features. Besides, bioethanol which will be obtained from low cost raw materials will be more attractive. Bioethanol produced from lignocellulosic biomass sources, such as agricultural residues, offers unique environmental and economic benefits.
  • Conference Object
    In Vitro Antitumor Activity of Sarcopoterium Spinosum Leaf Extract With Bioactive Natural Compounds
    (Elsevier, 2013) Süngüç, Ceren; Erdoğan, İpek; Uslu, Mehmet Emin; Bayraktar, Oğuz
    Cancer cell lines cause generation of reactive oxygen species and free radicals at high levels (Wang and Yi, 2008). Then generated free radicals lead to breakdown of the structure of DNA, lipid or protein (Gul et al., 2011). When plant extracts including antioxidant phytochemicals are exposed to the redox reactions, the harmful effects of free radicals are effectively prevented. The aim of present research was to evaluate the antitumor potential of the extract derived from Sarcopoterium spinosum leaves. The leaves of S. spinosum were collected in Izmir, Turkey. Total phenol content of ethanolic.
  • Conference Object
    Investigation of Anti-Tumoral Activity of Cistus Creticus Extract Against Pc-3 Cell Line
    (Elsevier, 2013) Erdoğan, İpek; Süngüç, Ceren; Uslu, Mehmet Emin; Bayraktar, Oğuz
    Recent studies have revealed that plant extracts show cytotoxic activities against cancer cell lines by ceasing cell division in particular phases (Xu et al., 2012, Yıldırım et al., 2013). Expression of specific genes was found to be activated according to pathway in which cell death occurs. Objective of this study was to identify antitumoural effect of Cistus creticus, which is a perennial shrub, found in Mediterranean region, against prostate cancer cell line by measuring the cytotoxic activities and apoptotic gene expression levels.
  • Article
    Citation - WoS: 21
    Citation - Scopus: 21
    Development of Adjuvant Nanocarrier Systems for Seasonal Influenza a (h3n2) Vaccine Based on Astragaloside Vii and Gum Tragacanth (aps)
    (Elsevier, 2019) Yakuboğulları, Nilgün; Genç, Rukan; Coven, Fethiye; Nalbantsoy, Ayşe; Bedir, Erdal
    Adjuvants are chemical/biological substances that are used in vaccines to increase the immunogenicity of antigens. A few adjuvants have been developed for use in human vaccines because of their limitations including lack of efficacy, unacceptable local or systemic toxicity, the difficulty of manufacturing, poor stability, and high cost. For that reasons, novel adjuvants/adjuvant systems are under search. Astragaloside VII (AST-VII), isolated from Astragalus trojanus, exhibited significant cellular and humoral immune responses. The polysaccharides (APS) obtained from the roots of Astragalus species have been used in traditional Chinese medicine and possess strong immunomodulatory properties. In the present study, the immunomodulatory effects of a newly developed nanocarrier system (APNS: APS containing carrier) and its AST-VII containing formulation (ANS: AST-VII + APNS), on seasonal influenza A (H3N2) vaccine were investigated. Inactivated H3N2 alone or its combinations with test compounds/formulations were intramuscularly injected into Swiss albino mice. Four weeks after immunization, the immune responses were evaluated in terms of antibody and cytokine responses as well as splenocyte proliferation. APNS demonstrated Th2 mediated response by increasing IgG1 antibody titers, whereas ANS showed response towards Th1/Th2 balance and Th17 by producing of IFN-gamma, IL-17A and IgG2a. Based on these results, we propose that APNS and ANS are good candidates to be utilized in seasonal influenza A vaccines as adjuvants/carrier systems. (C) 2019 Elsevier Ltd. All rights reserved.
  • Article
    Citation - WoS: 24
    Citation - Scopus: 23
    Polyethers Isolated From the Marine Actinobacterium Streptomyces Cacaoi Inhibit Autophagy and Induce Apoptosis in Cancer Cells
    (Elsevier, 2019) Khan, Nasar; Yılmaz, Sinem; Aksoy, Semiha; Uzel, Ataç; Tosun, Çiğdem; Ballar Kırmızıbayrak, Petek; Bedir, Erdal
    Polyether compounds, a large group of biologically active metabolites produced by Streptomyces species have been reported to show a variety of bioactivity such as antibacterial, antifungal, antiparasitic, antiviral, and tumour cell cytotoxicity. Since some of these compounds target cancer stem cells and multi-drug resistant cancer cells, this family of compounds have become of high interest. In this study, three polyether-type metabolites (1-3), one of which was a new natural product (3), were isolated from the marine derived Streptomyces cacaoi via antimicrobial activity-guided fractionation studies. As several polyether compounds with structural similarity such as monensin have been linked with autophagy and cell death, we first assessed the cytotoxicity of these three compounds. Compounds 2 and 3, but not 1, were found to be cytotoxic in several cell lines with a higher potency towards cancer cells. Furthermore, 2 and 3 caused accumulation of both autophagy flux markers LC3-II and p62 along with cleavage of caspase-3, caspase-9 and poly (ADP-ribose) polymerase 1 (PARP-1). Interestingly, prolonged treatment of the compounds caused a dramatic downregulation of the proteins related to autophagasome formation in a dose dependent manner. Our findings provide insights on the molecular mechanisms of the polyether-type polyketides, and signify their potency as chemotherapeutic agents through inhibiting autophagy and inducing apoptosis.
  • Conference Object
    Akt Inhibitor Arq 092 and Sorafenib Additively Inhibit Progression of Hepatocellular Carcinoma and Improve Immune System in Cirrhotic Rat Model
    (Elsevier, 2017) Jilkova, Z. M.; Zeybek Kuyucu, Ayça; Kurma, K.; Pour, S. T. A.; Roth, G. S.; Abbadessa, G.; Decaens, T.
    Background and Aims: Hepatocellular carcinoma (HCC) is often diagnosed at advanced stages with limited number of therapeutic options. Longer exposure to classical treatment of advanced HCC, sorafenib, often over-activates AKT pathway, leading to HCC resistance. Moreover, AKT pathway itself is activated in almost half of HCC cases. Therefore, we investigated the efficacy of combination of Sorafenib with allosteric Akt inhibitor ARQ 092 in a DEN-induced cirrhotic rat model with HCC.