Okuklu, Burcu
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Okuklu, B.
Okuklu, B
Okuklu, Burcu Ozturk
Okuklu, Burcu Öztürk
Okuklu Ozturk, Burcu
Okuklu Öztürk, Burcu
Ozturk, Burcu Okuklu
Öztürk, Burcu Okuklu
Okuklu, B
Okuklu, Burcu Ozturk
Okuklu, Burcu Öztürk
Okuklu Ozturk, Burcu
Okuklu Öztürk, Burcu
Ozturk, Burcu Okuklu
Öztürk, Burcu Okuklu
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Email Address
burcuokuklu@iyte.edu.tr
Main Affiliation
03.08. Department of Food Engineering
Status
Current Staff
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Sustainable Development Goals
1NO POVERTY
0
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2ZERO HUNGER
1
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3GOOD HEALTH AND WELL-BEING
0
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4QUALITY EDUCATION
1
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5GENDER EQUALITY
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6CLEAN WATER AND SANITATION
1
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7AFFORDABLE AND CLEAN ENERGY
1
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8DECENT WORK AND ECONOMIC GROWTH
0
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9INDUSTRY, INNOVATION AND INFRASTRUCTURE
2
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10REDUCED INEQUALITIES
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11SUSTAINABLE CITIES AND COMMUNITIES
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12RESPONSIBLE CONSUMPTION AND PRODUCTION
1
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13CLIMATE ACTION
1
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14LIFE BELOW WATER
1
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15LIFE ON LAND
1
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16PEACE, JUSTICE AND STRONG INSTITUTIONS
0
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17PARTNERSHIPS FOR THE GOALS
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Documents
4
Citations
179
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4
This researcher does not have a WoS ID.
Scholarly Output
6
Articles
4
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6703/3492
Supervised MSc Theses
1
Supervised PhD Theses
1
WoS Citation Count
159
Scopus Citation Count
179
Patents
0
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0
WoS Citations per Publication
26.50
Scopus Citations per Publication
29.83
Open Access Source
6
Supervised Theses
2
| Journal | Count |
|---|---|
| Foodborne Pathogens and Disease | 1 |
| Food Control | 1 |
| Journal of Dairy Research | 1 |
| LWT - Food Science and Technology | 1 |
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6 results
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Now showing 1 - 6 of 6
Article Citation - WoS: 40Citation - Scopus: 38Homofermentative Lactic Acid Bacteria of a Traditional Cheese, Comlek Peyniri From Cappadocia Region(Cambridge University Press, 2005) Bulut, Çisem; Güneş, Hatice; Okuklu, Burcu; Harsa, Hayriye Şebnem; Kılıç, Sevda; Çoban, Hatice S.; Yenidünya, Ali FazılComlek peyniri is a typical artisanal cheese in Central Anatolia. This type of cheese was made by using the indigenous lactic acid bacteria (LAB) flora of cow or ewes' milk. Majority of the samples were taken from fresh cheese because the aim was to isolate homofermentative LAB. Initially 661 microbial isolates were obtained from 17 cheese samples. Only 107 were found to be homofermentative LAB. These isolates were selected and identified by using both phenotypic and molecular methods. Phenotypic identification included curd formation from skim milk, catalase test, Gram staining and light microscopy, growth at different temperatures and salt concentrations, arginine hydrolysis, gas production from glucose, and carbohydrate fermentation. Molecular identification was based on the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the 16S rRNA gene-ITS (internally transcribed spacer) region. By combining the phenotypic and molecular identification results, isolates belonging to each of the following genera were determined at species or subspecies level: 54 Lactococcus lactis subsp. lactis, 21 Enterococcus faecium, 3 Ec. faecalis, 2 Ec. durans, 10 Ec. sp., 15 Lactobacillus paracasei subsp. paracasei, and 2 Lb. casei strains. Technological characterisation was also performed by culturing each of the strains in UHT skim milk, and by monitoring pH change and lactic acid production at certain time intervals through the 24 h incubation. Results of the technological characterisation indicated that 33% of the isolates (35 strains) were capable of lowering the pH of UHT milk below 5.3 after 6 h incubation at 30°C. Thirty four of these strains were Lc. lactis subsp. lactis, and only one was an Ec. faecium strain.Article Citation - WoS: 46Citation - Scopus: 53Extracellular Enzyme Production and Enterotoxigenic Gene Profiles of Bacillus Cereus and Bacillus Thuringiensis Strains Isolated From Cheese in Turkey(Elsevier Ltd., 2009) Molva, Çelenk; Sudağıdan, Mert; Okuklu, BurcuThe aim of the present study was to investigate the biochemical characteristics, extracellular enzyme production and enterotoxigenic genes contents of 6 Bacillus cereus and 22 Bacillus thuringiensis strains, isolated from 100 cheese samples in Turkey. Crystal morphologies of B. thuringiensis strains were found either spherical (n = 12) or spherical and irregular-shaped (n = 10) by phase contrast microscopy. B. cereus and B. thuringiensis strains were found to produce extracellular enzymes, respectively: gelatinase (83% and 91%), DNase (83% and 77%), lecithinase (83% and 95%), protease on skim milk agar (100% and 100%), protease on milk agar (100% and 91%), protease on casein agar (83% and 77%), xylanase (100% and 45%), and cellulase (0% and 41%), and amylase (83% and 27%). All of the strains, except for Bt-D1, hydrolyzed Tween 20 (96%), but not Tween 80 or tributyrin. Pectinolytic activity was obtained to be the least frequent (4%). PCR analysis showed that all strains contained nheA, nheB, nheC and hblD genes. The hblA and hblC genes were present in 2 and 4 of B. thuringiensis strains, respectively. The bceT gene was detected in 1 B. cereus and 9 B. thuringiensis strains. The entFM gene was detected more frequently in B. thuringiensis (82%) than in B. cereus strains (50%). To our knowledge, this is the first report about the isolation and identification of enterotoxigenic B. cereus and B. thuringiensis strains from cheese samples in Turkey.Article Citation - WoS: 34Citation - Scopus: 39High Genetic and Phenotypic Variability of Streptococcus Thermophilus Strains Isolated From Artisanal Yuruk Yoghurts(Academic Press Inc., 2014) Erkuş, Oylum; Okuklu, Burcu; Yenidünya, Ali Fazıl; Harsa, ŞebnemStreptococcus thermophilus is a commonly used starter bacterium in dairy industry. It reduces the pH of milk rapidly and equilibrates the medium for the growth of Lactobacillus delbrueckii subsp. bulgaricus during yoghurt fermentation. Efforts to increase the diversity of artisanal yoghurt starters are not only important to bring new strains with novel and desirable characteristics, but also for the preservation of natural diversity which diminishes with the overuse and spread of industrial starters to natural resources. In the present study, 14 artisanal yoghurt samples were processed for the isolation of promising strains for yoghurt starter culture production and 66 strains were subsequently characterized. They were all identified as S. thermophilus using species-specific PCR and 16S rRNA gene sequencing. Genotypic diversity at the strain level was investigated by pulsed field gel electrophoresis (PFGE), and 22 homology groups were obtained. Further phenotypic characterization unearthed a significant phenotypic heterogeneity within homology groups, mostly with atypical novel character. Only 7 out of 66 strains showed S. thermophilus type-strain like phenotypic traits. Majority of the isolates were determined to be protease positive and fast milk acidifier to be used as yoghurt starter culture.Doctoral Thesis Isolation, Characterization, and Screening Probiotic Properties of Artisanal Yoghurt Starter Strains From Urla Region(Izmir Institute of Technology, 2014) Okuklu, Burcu; Harsa, Hayriye Şebnem; Yenidünya, A.FazılProbiotics are bacteria that help to maintain the natural balance of microflora in the intestine. The largest group of probiotic bacteria in the intestine is lactic acid bacteria, found in yoghurt with live culture. In this study, 13 different artisanal yoghurt samples were collected from Urla region to isolate artisanal starter strains. These isolated strains were firstly identified and characterized by using basic biochemical, physiological and probiotic characterization methods. Among 453 LAB strains in total, 5 Streptecoccus thermophilus and 26 Lactobacillus delbrueckii ssp. bulgaricus isolates resulted as probiotic candidates. PCR-RFLP and PFGE-RFLP methods were used for the differentiation of probiotic candidates. Probiotic features of strains were screened by using bile salt tolerance, bile salt deconjugation, and cholesterol assimilation, transit tolerance to gastrointestinal tract, antibiotic resistances, autoaggregation, cell surface hydrophobicity, antimicrobial activities, adhesion abilities, and growth capacity with prebiotics. The results showed that, the isolated strains gave different properties against these tests; but all of them were acid and bile resistant, had ability to adhesion Caco-2 cell lines, and grown very well with prebiotic sources. They had also antimicrobial activity against Escherichia coli and Listeria innocua. Further studies were conducted; probiotic yoghurts were produced by using probiotic candidates. Sixteen starter combinations were made using the two cocci and eight bacilli isolates, and thus these yoghurt products were characterized by using physical, chemical, rheological, and organoleptic methods. Acetaldehyde content of yoghurt samples varied between 5.61 and 15.38 mg/L and apparent viscosity values ranged from 261 and 608 m Pa.s. Hardness value of samples ranged from 3.81 and 6.71 N. Yoghurt starter having probiotic features is an advantage for dairy industry to produce large amount of functional yoghurt. In this study, a good number of cocci and bacilli were paired as probiotic/starter strain combinations that could perfectly be used for the production of functional yoghurt for dairy industry.Article Citation - WoS: 39Citation - Scopus: 49Prevalence and Antibiotic Resistance of Foodborne Staphylococcus Aureus Isolates in Turkey(Mary Ann Liebert Inc., 2011) Aydın, Ali; Muratoğlu, Karlo; Sudağıdan, Mert; Bostan, Kamil; Okuklu, Burcu; Harsa, ŞebnemIn this study, 154 Staphylococcus aureus isolates were detected from 1070 food samples (14.4%) collected from seven cities in Turkey. Antimicrobial susceptibility testing against 21 antibiotics was performed by agar disk diffusion method, and those isolates resistant to any antibiotic were further analyzed to determine minimum inhibitory concentration by E-test and polymerase chain reaction analysis of vanA and mecA genes. According to disk diffusion test results, a total of 139 strains were resistant to at least one tested antibiotic, with 39 (25.3%) strains being multidrug resistant (MDR) and the other 15 strains being susceptible to all antibiotics. Penicillin G, linezolid, erythromycin, and tetracycline took up 71.4%, 23.4%, 18.2%, and 15.6% of the tested strains, respectively. In addition, all of the strains were susceptible to vancomycin, oxacillin, cefoxitin, and imipenem. Only one strain (S158B) was resistant to both teicoplanin and cefazolin. On the other hand, the presence of vanA and mecA genes was not detected in the strains. Pulsed-field gel electrophoresis analysis was used to identify genetic-relatedness of the MDR strains. It is noteworthy that some strains from different sources showed 100% homology; however, some of MDR strains were found unrelated with 60% or less homology. The high diversity observed in pulsed-field gel electrophoresis results indicated the possible contamination of S. aureus from different sources and routes.Master Thesis Investigation of Chromosomal and Plasmid Dna Profiles of Lactococcus Lactics Ssp. Lactis(Izmir Institute of Technology, 2005) Okuklu, Burcu; Güneş, HaticeLactic acid bacteria used in the manufacturing of cheese and other fermented milk products are known as starter cultures. Starters play an important role in the sensory properties of fermented milk products by lactic acid production which influences desirable quality characteristics. In dairy industry, diversity of starter strains of Lactococcus lactis is very limited. There is a considerable demand for novel starters with technologically desirable properties. Gene products responsible for lactose fermentation are encoded on plasmid DNA. Therefore, the plasmid stability of lactic acid bacteria is an important industrial property for starter culture. In the previous study, Lc. lactis ssp. lactis strains were isolated from an artisanal "comlek peyniri'" from Cappodocia and these strains were selected as good acid producers. The aim of this study was to investigate intraspecific diversity of 54 Lc. lactis ssp. lactis by choromosomal and plasmid profiling. In addition, the plasmid stability of 35 artisanal starters was determined by the examination -galactosidase activity. First of all, partial DNA sequencing of the 16S rRNA genes was carried out in order to confirm that the previously identified isolates were Lc. lactis ssp. lactis. The partial DNA sequencing and blast search results indicated that representative isolates showed 90 % homology with Lactococcus lactis. Based on chromosome and plasmid profiling results, 35 Lactococcus lactis artisanal starters exhibiting good acidifying activities were classified into ten different genotypes and nine plasmid groups. None of the reference strains of Lactococcus lactis ssp. lactis and Lc. lactis ssp. cremoris and Lc. lactis ssp. lactis biovar. diacetylactis were included into these genotypes and plasmid groups. The rest of the 17 isolates which were defined as low acid producers by technological methods were clustered into thirteen unique genotypes and seven plasmid groups. Only four of the isolates were found to contain the most stable plasmids by instability studies and these kept their lactase activities during prolonged subculturing. As a result, chromosome and plasmid DNA profiling allowed us to classify artisanal starter strains in certain groups. The strains with stable and instable plasmids will serve us to characterize and improve technological features of these artisanal starter strains in future studies.