Şahin, Çağatay

Profile URL
https://hdl.handle.net/11147/16277
Name Variants
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Email Address
Main Affiliation
01. Izmir Institute of Technology
Status
Current Staff
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ORCID ID
Scopus Author ID
Turkish CoHE Profile ID
Google Scholar ID
WoS Researcher ID

Sustainable Development Goals

NO POVERTY1
NO POVERTY
0
Research Products
ZERO HUNGER2
ZERO HUNGER
0
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GOOD HEALTH AND WELL-BEING3
GOOD HEALTH AND WELL-BEING
2
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QUALITY EDUCATION4
QUALITY EDUCATION
0
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GENDER EQUALITY5
GENDER EQUALITY
0
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CLEAN WATER AND SANITATION6
CLEAN WATER AND SANITATION
0
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AFFORDABLE AND CLEAN ENERGY7
AFFORDABLE AND CLEAN ENERGY
0
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DECENT WORK AND ECONOMIC GROWTH8
DECENT WORK AND ECONOMIC GROWTH
0
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INDUSTRY, INNOVATION AND INFRASTRUCTURE9
INDUSTRY, INNOVATION AND INFRASTRUCTURE
0
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REDUCED INEQUALITIES10
REDUCED INEQUALITIES
0
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SUSTAINABLE CITIES AND COMMUNITIES11
SUSTAINABLE CITIES AND COMMUNITIES
0
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RESPONSIBLE CONSUMPTION AND PRODUCTION12
RESPONSIBLE CONSUMPTION AND PRODUCTION
0
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CLIMATE ACTION13
CLIMATE ACTION
0
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LIFE BELOW WATER14
LIFE BELOW WATER
0
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LIFE ON LAND15
LIFE ON LAND
0
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PEACE, JUSTICE AND STRONG INSTITUTIONS16
PEACE, JUSTICE AND STRONG INSTITUTIONS
0
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PARTNERSHIPS FOR THE GOALS17
PARTNERSHIPS FOR THE GOALS
0
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This researcher does not have a Scopus ID.
This researcher does not have a WoS ID.
No records found in other affiliations.
Scholarly Output

2

Articles

1

Views / Downloads

7892/242

Supervised MSc Theses

1

Supervised PhD Theses

0

WoS Citation Count

5

Scopus Citation Count

5

Patents

0

Projects

0

WoS Citations per Publication

2.50

Scopus Citations per Publication

2.50

Open Access Source

1

Supervised Theses

1

JournalCount
Brazilian Archives of Biology and Technology1
Current Page: 1 / 1

Scopus Quartile Distribution

Competency Cloud

GCRIS Competency Cloud
Author of Publication: search.filters.isAuthorOfPublication.af343464-8922-47bb-8e14-2e5b15357aed

Scholarly Output Search Results

Now showing 1 - 2 of 2
  • Master Thesis
    Neuroblastoma Ras (NRAS) Mutasyonlu Melanomda Negative Regulator Of Antiviral Response (NRAV) Uzun Kodlamayan RNA'nın Tip-2 İnterferon Yanıtındaki Rollerinin İncelenmesi ve Uzun Kodlamayan RNA İfade Örüntülerinin İmmünoterapi Yanıtıyla İlişkilerinin Araştırılması
    (2025) Şahin, Çağatay; Ekiz, Hüseyin Atakan; Ekiz, Hüseyin Atakan; Güner, Şerife Ayaz; 01. Izmir Institute of Technology; 04. Faculty of Science; 04.03. Department of Molecular Biology and Genetics
    Bu çalışma, uzun kodlamayan RNA (lncRNA) NRAV'ın tip II interferon (IFN-γ) sinyal yolaklarını düzenlemedeki rolünü ve bunun melanoma biyolojisi ile immünoterapi etkinliği üzerindeki etkilerini incelemektedir. NRAS mutasyonuna sahip SKMEL147 melanoma hücre hattı kullanılarak, IFN-γ uyarımı öncesi ve sonrası koşullarda interferonla uyarılan genlerin (ISG) ekspresyonu, hücre proliferasyonu ve hücre ölümü üzerindeki değişiklikleri değerlendirmek amacıyla NRAV aşırı ifade eden hücre modelleri oluşturulmuştur. NRAV'ın aşırı ifadesi, IFIT2, IRF1 ve PD-L1 gibi kritik ISG'leri baskılayarak IFN-γ–STAT1 sinyal yolunun inhibe edildiğini göstermiştir. Fonksiyonel olarak, NRAV hücre büyümesini azaltmış ve hücre ölümünü nekroza kaydırmıştır; bu bulgu koloni oluşum deneyleri ve akış sitometrisi ile doğrulanmıştır. RNA pulldown sonrası yapılan proteomik analiz, NRAV ile ilişkili proteinlerin otofaji, translasyon ve mitokondriyal stres yanıtlarında rol aldığını ortaya koyarak, NRAV'ın hücre kaderini post-transkripsiyonel düzeyde düzenleyebileceğini göstermiştir. Melanoma immünoterapisi veri setlerine uygulanan makine öğrenmesi, LINC02273, LINC01833 ve MIR210HG gibi immünoterapiye yanıtla ilişkili olabilecek çok sayıda lncRNA'yı belirlemiş, ZFAT-AS1 ve CSRP3-AS1 gibi yeni adayları da ortaya koymuştur. Bu bulgular, NRAV'ın hem IFN aracılı bağışıklığı baskılayıcı hem de nekrotik hücre ölümünü düzenleyici ikili işlevine dikkat çekmekte olup, onu gelecekteki mekanistik ve terapötik çalışmalar için potansiyel bir hedef haline getirmektedir. Bu araştırma, melanomada bağışıklık düzenleyici lncRNA'ların anlaşılmasını derinleştirerek, immünoterapi direncinde biyobelirteç ve hedef keşfi için yeni yollar açmaktadır.
  • Article
    Citation - WoS: 5
    Citation - Scopus: 5
    Angelica Sylvestris and Delphinium Staphisagria Extracts Induces Antiproliferation Through Caspase-Mediated Apoptosis on Human Cancer Cells
    (Instituto de Tecnologia do Parana, 2022) Akgün, Oğuzhan; Şahin, Çağatay; Şahin, Çağatay; Çelikler, Serap; Arı, Ferda; 01. Izmir Institute of Technology
    Angelica sylvestris and Delphinium staphisagria are medicinal and aromatic herbs with a long history in medicine and food industry. In this study, we have investigated anti-cancer activity of Angelica sylvestris and Delphinium staphisagria extracts on various cell lines of lung (A549), breast (MCF-7), colon (HT-29), and cervix (HeLa) origin. Also, cytotoxicity was tested on human healthy bronchial epithelial (BEAS-2B) cells. In vitro experiments showed that plant extracts suppressed cell growth and proliferation at low concentrations by reducing cell viability on cancer cells in a time and concentration-dependent manner. It was observed that Angelica sylvestris was more effective in HT-29 and HeLa cells and Delphinium staphisagria in A549 and MCF-7 cells by suppressing cell proliferation and increasing cell death. Cell death mode (apoptosis/necrosis) was investigated via fluorescent imaging, caspase-cleaved cytokeratin 18, activated caspase-3, and cleaved-PARP (poly (ADP-ribose) polymerase). In order to evaluate the cell death mode by plant extracts apoptotic markers were investigated by fluorescence staining. Delphinium staphisagria extract (50-200 μg/mL) caused a decrease in cell density in A549 and MCF-7 cells compared to untreated controls. A similar situation was observed in HT-29 and HeLa cell lines when treated with ASE. As a result, Delphinium staphisagria extracts induced apoptosis in A549 and MCF-7, while Angelica sylvestris extracts induced apoptosis in HT-29 and HeLa cancer cells